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Development of in situ hybridisation using 16S rRNA gene to monitor black-lip pearl oyster, Pinctada margaritifera, larvaein plankton samples

Published online by Cambridge University Press:  15 April 2011

Yoann Thomas ,
Corinne Belliard ,
Pierre Garen ,
Yannick Gueguen  and
Caroline Montagnani
Yoann Thomas
Affiliation:
IFREMER, Centre océanologique du Pacifique, BP 7004, 98719 Taravao, French Polynesia
Corinne Belliard
Affiliation:
IFREMER, Centre océanologique du Pacifique, BP 7004, 98719 Taravao, French Polynesia
Pierre Garen
Affiliation:
IFREMER, Centre océanologique du Pacifique, BP 7004, 98719 Taravao, French Polynesia
Yannick Gueguen
Affiliation:
IFREMER, Centre océanologique du Pacifique, BP 7004, 98719 Taravao, French Polynesia
Caroline Montagnani*
Affiliation:
IFREMER, Centre océanologique du Pacifique, BP 7004, 98719 Taravao, French Polynesia
*
a Corresponding author: [email protected]
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Abstract

In French Polynesia, the black-lip pearl oyster Pinctada margaritifera has been farmed to produce pearls since the 1980s, forming the basis of a major industry. The sustainability of this activity relies on spat collection in the lagoons. However, pearl oyster spat can be difficult to identify for the evaluation of stock variations. It is especially hard to distinguish Pinctada spp. larvae at a very early stage of development. In the present study, a whole-mount in situ hybridisation (ISH) technique was developed to allow the discrimination of larvae of closely-related pearl oyster species found in the French Polynesian atolls. Using specific ribosomal 16S-DNA sequence data, we were able to successfully differentiate between Pinctada margaritifera and Pinctada maculata larvae from 5 to 13 days old. This is the first description of a non-destructive method allowing bivalve larvae discrimination between species within this genus. The method allowed us to successfully identify P. margaritifera larvae in natural plankton samples. This result is a key step needed to develop monitoring of P. margaritifera larval distribution in French Polynesian lagoons, a procedure which will increase spat collection efficiency and ensure sustainable development of pearl oyster farming.

Keywords

Whole larvae in situ hybridisation16S rRNAPlanktonSpecies identificationPearl oysterPinctada margaritifera
Type
Research Article
Information
Aquatic Living Resources , Volume 24 , Issue 1 , January 2011 , pp. 27 - 34
Copyright
© EDP Sciences, IFREMER, IRD 2011

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