Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-24T04:24:28.726Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of domoic acid, the amnesic shellfish toxin,in the digestive gland of Eledone cirrhosa and E. moschata (Cephalopoda, Octopoda) from the Portuguese coast

Published online by Cambridge University Press:  23 December 2005

Pedro R. Costa ,
Rui Rosa ,
João Pereira  and
Maria Antónia M. Sampayo
Pedro R. Costa
Affiliation:
Departamento de Ambiente Aquático, IPIMAR, Avenida de Brasília, 1449-006 Lisboa, Portugal
Rui Rosa
Affiliation:
Departamento de Inovação Tecnológica e Valorização dos Produtos da Pesca, IPIMAR, Avenida de Brasília, 1449-006 Lisboa, Portugal
João Pereira
Affiliation:
Departamento de Recursos Marinhos, IPIMAR, Avenida de Brasília, 1449-006 Lisboa, Portugal
Maria Antónia M. Sampayo
Affiliation:
Departamento de Ambiente Aquático, IPIMAR, Avenida de Brasília, 1449-006 Lisboa, Portugal
Get access

Abstract

Domoic acid (DA), the toxin responsible for the illness known as amnesic shellfish poisoning (ASP), is an algal toxin produced naturally by some species of the diatom genus Pseudo-nitzschia. The toxin has been detected in a diverse array of marine organisms from copepods to whales. Cephalopods, which are important members of the food chain and active predators of known toxin vectors such as bivalves, crabs and some fishes, have just recently been implicated in DA transfer or accumulation. Here we present data showing detectable values of DA determined by HPLC-UV (high-performance liquid chromatography and ultraviolet detection) and confirmed by HPLC-MS (mass spectrometric detection) in two octopus species collected along the Portuguese continental coast: Eledone cirrhosa and E. moschata. Domoic acid was frequently detected in the digestive gland of E. moschata and occasionally reached concentrations exceeding 100 µg g−1. In contrast, E. cirrhosa contained lower concentrations of DA on the few occasions that it was detected. This suggests that E. moschata is a potential vector for DA transfer to higher trophic levels in the coastal marine food web, not excluding humans. These data, combined with known aspects of the life history of the species, are a necessary step towards achieving an understanding of the accumulation of phycotoxins in cephalopods.

Keywords

Domoic acidEledone moschataEledone cirrhosaAmnesic shellfish poisoningNE Atlantic
Type
Research Article
Information
Aquatic Living Resources , Volume 18 , Issue 4: Environmental Effects on Cephalopod Life History and Fisheries , October 2005 , pp. 395 - 400
Copyright
© EDP Sciences, IFREMER, IRD, 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Arévalo F.F., Bermúdez M., Salgado C., 1998, ASP toxicity in scallops: individual variability and tissue distribution. In: Reguera B., Blanco J., Fernández M.L., Wyatt T. (Eds.) Harmful algae. Xunta de Galicia and the IOC of UNESCO, Paris, pp. 499-502.
Bargu, S., Powell, C.L., Coale, S.L., Busman, M., Doucette, G.J., Silver, M.W., 2002, Krill: a potential vector for domoic acid in marine food webs. Mar. Ecol. Prog. Ser. 237, 209-216. CrossRef
Bates, S.S., Bird, C.J., de Freitas, A.S.W., Foxall, R., Gilgan, M., Hanic, L.A., Johnson, G.R., McCulloch, A.W., Odense, P., Pocklington, R., Quilliam, M.A., Sim, P.G., Smith, J.C., Subba Rao, D.V., Todd, E.C.D., Walter, J.A., Wright, J.L.C., 1989, Pennate diatom Nitzschia pungens as the primary source of domoic acid, a toxin in shellfish from eastern Prince Edward Island, Canada. Can. J. Fish. Aquat. Sci. 46, 1203-1215. CrossRef
Belcari, P., Sartor, P., 1999, Eledone cirrhosa. In: Relini G., Bertran J.A., Zambroni A. (Eds.) Synthesis of the knowledge on bottom fishery resources in central Mediterranean (Italy and Corsica). Biol. Mar. Medit. 6, 737-746.
Blanco, C., Salomón, O., Raga, J.A., 2001, Diet of the bottlenose dolphin (Tursiops truncatus) in the western Mediterranean Sea. J. Mar. Biol. Assoc. UK 81, 1053-1058. CrossRef
Boucaud-Camou, E., Boucher-Rodoni, R., Mangold, K., 1976, Digestive absorption in Octopus vulgaris (Cephalopoda, Octopoda). J. Zool. 179, 261-271. CrossRef
Boucher-Rodoni, R., Mangold, K., 1977, Experimental study of digestion in Octopus vulgaris (Cephalopoda: Octopoda). J. Zool. 183, 505-515 CrossRef
Boucher-Rodoni R., Boucaud-Camou E., Mangold K., 1987, Feeding and digestion. In: Boyle P.R. (Eds.) Cephalopod Life Cycles. Comparative Reviews. London, Academic Press, Vol. 2, pp. 85-108.
Boyle P.R., 1983, Eledone cirrhosa. In: Boyle P.R. (Ed.) Cephalopod life cycles. London, Academic Press, Vol 1., pp. 365-386.
Boyle, P.R., 1986, A descriptive ecology of Eledone cirrhosa (Mollusca: Cephalopoda) in Scottish waters. J. Mar. Biol. Assoc. UK 66, 855-865. CrossRef
Boyle, P., Boletzky, S., 1996, Cephalopod populations: definition and dynamics. Phil. Trans. R. Soc. London B 351, 985-1002. CrossRef
Costa, P.R., Garrido, S., 2004, Domoic acid accumulation in the sardine (Sardina pilchardus) and its relationship to Pseudo-nitzschia diatom ingestion. Mar. Ecol. Prog. Ser. 284, 261-268. CrossRef
Costa, P.R., Rodrigues, S., Botelho, M.J., Sampayo, M.A.M., 2003, A potential vector of domic acid: the swimming crab Polybius henslowii Leach (Decapoda-Brachyura). Toxicon 42, 135-141. CrossRef
Costa, P.R., Rosa, R, Sampayo, M.A.M., 2004, Tissue distribution of the amnesic shellfish toxin, domoic acid, in Octopus vulgaris from the Portuguese coast. Mar. Biol. 144, 971-976. CrossRef
Costa, P.R., Rosa, R, Duarte-Silva, A., Brotas, V., Sampayo, M.A.M., 2005, Accumulation, transformation and tissue distribution of domoic acid, the amnesic shellfish poisoning toxin, in the common cuttlefish, Sepia officinalis. Aquat. Toxicol. 74, 82-91
Cusack, C.K., Bates, S.S., Quilliam, M.A., Patching, J.W., Raine, R., 2002, Confirmation of domoic acid production by Pseudo-nitzschia australis (Bacillariophyceae) isolated from Irish waters. J. Phycol. 38, 1106-1112 CrossRef
Dortch, Q., Robichaux, R., Pool, S., Milsted, D., Mire, G., Rabalais, N.N., Soniat, T.M., Fryxell, G.A., Turner, R.E., Parsons, M.L., 1997, Abundance and vertical flux of Pseudo-nitzschia in the northern Gulf of Mexico. Mar. Ecol. Prog. Ser. 146, 249-264. CrossRef
Garrison, D.L., Conrad, SM., Eilers, P.P., Waldron, E.M., 1992, Confirmation of domoic acid production by Pseudonitzschia australis (Bacillariophyceae) cultures. J. Phycol. 28, 604-607. CrossRef
Grisley, M.S., Boyle, P.R., Pierce, G.J., Key, L.N., 1999, Factors affecting prey handling in lesser octopus (Eledone cirrhosa) feeding on crabs (Carcinus maenas). J. Mar. Biol. Assoc. UK 79, 1085-1090. CrossRef
Guerra A., 1992, Mollusca Cephalopoda. Fauna Iberica. Vol. 1. Museo Nacional de Ciencias Naturales – CSIC, Madrid.
Lefebvre, K.A., Powell, C.L., Busman, M., Doucette, G.J., Moeller, P.D.R., Silver, J.B., Miller, P.E., Hughes, M.P., Singaram, S., Silver, M.W., Tjeerdema, R.S., 1999, Detection of domoic acid in northern anchovies and California sea lions associated with an unusual mortality event. Nat. Toxins 7, 85-92. 3.0.CO;2-Q>CrossRef
Lincoln, J.A., Turner, J.T., Bates, S.S., Léger, C., Gauthier, D.A., 2001, Feeding, egg production and egg hatching success of the copepods Acartia tonsa and Temora longicornis on diets of the toxic diatom Pseudo-nitzschia multiseries and the non-toxic diatom Pseudo-nitzschia pungens. Hydrobiologia 453, 107-120. CrossRef
Mangold, K., 1983a, Food, feeding and growth in cephalopods. Mem. Nat. Mus. Victoria 44, 81-93.
Mangold K., 1983b, Eledone moschata. In: Boyle P.R. (Ed.) Cephalopod life cycles. London, Academic Press, Vol. 1, pp. 387-400.
Novaczek, I., Madhyastha, M.S., Ablett, R.F., Johnson, G., Nijjar, M.S., Sims, D.E., 1991, Uptake, disposition and depuration of domoic acid by blue mussels (Mytilus edulis). Aquat. Toxicol. 21, 103-118. CrossRef
O'Dor R.K., Weber M.J., 1987, Energy and nutrient flow. In: Boyle P.R. (Ed.) Cephalopod life cycles. Comparative reviews. London, Academic Press, Vol. 2, pp. 109-133.
Parsons, M.L., Dortch, Q., Turner, R.E., 2002, Sedimentological evidence of an increase in Pseudo-nitzschia (Bacillariophyceae) abundance in response to coastal eutrophication. Limnol. Oceanogr. 47, 551-558. CrossRef
Piatkowski, U., Pierce, G.J., Cunha, M.M., 2001, Impact of cephalopods in the food chain and their interaction with the environment and fisheries: an overview. Fish. Res. 52, 2-10.
Quilliam, M.A., Wright, J.L.C., 1989, The amnesic shellfish poisoning mystery. Anal. Chem. 61, 1053A-1060A. CrossRef
Quilliam, M.A., Xie, M., Hardstaff, W.R., 1995, Rapid extraction and cleanup for liquid chromatographic determination of domoic acid in unsalted seafood. J. AOAC Int. 78, 543-554.
Salman, A., Bilecenoglu, M., Güçlüsoy, H., 2001, Stomach content of two Mediterranean monk seals (Monachus monachus) from the Aegean Sea, Turkey. J. Mar. Biol. Assoc. UK 81, 719-720. CrossRef
Sánchez, P., 1981, Régime alimentaire d'Eledone cirrosa (Lamarck, 1798) (Mollusca, Cephalopoda) dans la mer Catalane. Rapp. Comm. Int. Mer. Médit. 27, 209-212.
Sánchez, P., Martín, P., 1993, Population dynamics of the exploited cephalopod species of the Catalan Sea (NW Mediterranean). Sci. Mar. 57, 153-159.
Sánchez, P., Maynou, F., Demestre, M., 2004, Modelling catch, effort and price in a juvenile Eledone cirrhosa fishery over a 10-year period. Fish. Res. 68, 319-327. CrossRef
Semmens, J.M., 2002, Changes in the digestive gland of the loliginid squid Sepioteuthis lessoniana (Lesson, 1830) associated with feeding. J. Exp. Mar. Biol. Ecol. 274, 19-39. CrossRef
Scholin, C.A., Gulland, F., Doucette, G.J., Benson, S., Busman, M., Chavez, F.P., Cordaro, J., DeLong, R., De Vogelaere, A., Harvey, J., Haulena, M., Lefebvre, K., Lipscomb, T., Loscutoff, S., Lowenstine, L.J., Martin, R.I., Miller, P.E., McLellan, W.A., Moeller, P.D.R., Powell, C.L., Rowles, T., Silvagnl, P., Silver, M., Spraker, T., Trainer, V., Van Dolah, F.M., 2000, Mortality of sea lions along the central California coast linked to a toxic diatom bloom. Nature 403, 80-84. CrossRef
Sierra Beltrán, A., Palafox-Uribe, M., Grajales-Montiel, J., Cruz-Villacorta, A., Ochoa, J.L., 1997, Sea bird mortality at Cabo San Lucas, Mexico: evidence that toxic diatom blooms are spreading. Toxicon 35, 447-453. CrossRef
Subba Rao, D.V., Quilliam, M.A., Pocklington, R., 1988, Domoic acid - a neurotoxic amino acid produced by the marine diatom Nitzschia pungens in culture. Can. J. Fish. Aquat. Sci. 45, 2076-2079.
Taylor, F.J.R., 1993, British Columbia: implications of the North American west coast experience. Harmful Algae News 6, 2-3.
Tester P.A., Pan Y., Doucette G.J., Scholin C.A., 2001, Accumulation of domoic acid activity in copepods. In: Hallegraeff G.M., Blackburn S.I., Bolch C.J., Lewis R.J. (Eds.) Harmful Algal Blooms 2000. Paris, Intergovernmental Oceanographic Commission of UNESCO, pp 418-421.
Todd, E.C.D., 1993, Domoic acid and amnesic shellfish poisoning- a review. J. Food. Protect. 56, 69-83. CrossRef
Vale, P., Sampayo, M.A.M., 2001, Domoic acid in Portuguese shellfish and fish. Toxicon 39, 893-904. CrossRef
Work T.M., Beale A.M., Fritz L., Quilliam M.A., Silver M., Buck K., Wright J.L.C., 1993, Domoic acid intoxication of brown pelicans and cormorants in Santa Cruz, California. In: Smayda T.J., Shimizu Y., (Eds.) Toxic phytoplankton blooms in the sea. Amsterdam, Elsevier Sci. Publ. BV, pp. 643-650.
Wright, J.L.C., Boyd, R.K., de Freitas, A.S.W., Falk, M., Foxall, R.A., Jamieson, W.D., Laycock, M.V., McCulloch, A.W., McInnes, A.G., Odense, P., Pathak, V., Quilliam, M.A., Ragan, M.A., Sim, P.G., Thibault, P., Walter, J.A., Gilgan, M., Richard, D.J.A., Dewar, D., 1989, Identification of domoic acid, a neuroexcitatory amino acid, in toxic mussels from eastern Prince Edward Island. Can. J. Chem. 67, 481-490. CrossRef