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Viability, growth and toxicity of Alexandrium catenellaand Alexandrium minutum (Dinophyceae) following ingestionand gut passage in the oyster Crassostrea gigas

Published online by Cambridge University Press:  17 May 2007

Mohamed Laabir ,
Zouher Amzil ,
Patrick Lassus ,
Estelle Masseret ,
Yosmina Tapilatu ,
Romain De Vargas  and
Daniel Grzebyk
Mohamed Laabir*
Affiliation:
Laboratoire Ecosystèmes Lagunaires, UMR CNRS-UM2 5119 case 093, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier, France
Zouher Amzil
Affiliation:
Laboratoire Phycotoxines, IFREMER, Centre de Nantes, BP 21105, 44311 Nantes, France
Patrick Lassus
Affiliation:
Laboratoire Phycotoxines, IFREMER, Centre de Nantes, BP 21105, 44311 Nantes, France
Estelle Masseret
Affiliation:
Laboratoire Ecosystèmes Lagunaires, UMR CNRS-UM2 5119 case 093, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier, France
Yosmina Tapilatu
Affiliation:
Laboratoire Ecosystèmes Lagunaires, UMR CNRS-UM2 5119 case 093, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier, France
Romain De Vargas
Affiliation:
Laboratoire Ecosystèmes Lagunaires, UMR CNRS-UM2 5119 case 093, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier, France
Daniel Grzebyk
Affiliation:
Laboratoire Ecosystèmes Lagunaires, UMR CNRS-UM2 5119 case 093, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier, France
*
[email protected]
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Abstract

Adult oysters Crassostrea gigas were experimentally fed with Alexandrium catenella and Alexandrium minutum which are responsible for recurrent toxic blooms in French coastal waters. C. gigas produced faeces and pseudofaeces containing intact and viable temporary pellicular cysts of these two Paralytic toxin producing species. When incubated in favourable conditions, these pellicular cysts were able to germinate at high rates (between 74 and 94%) and the resulting vegetative cells divided with growth rates close to the non- ingested cells (control). The toxin profile of the vegetative cells originated from the germinated temporary cysts was analyzed by liquid chromatography/fluorescence detection. Total toxin content of newly germinated cells was lower than that of cultured cells. Besides, cell contents of C2, B1, B2 and dcGTX3 toxins featured some changes. Our results suggest that the increased spreading of toxic dinoflagellates through the transfer of shellfish from contaminated towards pristine coastal areas cannot be ruled out. We also suggest that pellicular cysts and newly germinated cells could represent a potential way for the transfer of paralytic toxins toward the higher trophic levels.

Keywords

Alexandrium catenellaAlexandrium minutumCystsOystersParalytic Shellfish Poisoning
Type
Research Article
Information
Aquatic Living Resources , Volume 20 , Issue 1: New developments in coastal environment research: results from the national coastal environment program , January 2007 , pp. 51 - 57
Copyright
© EDP Sciences, IFREMER, IRD, 2007

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References

Bardouil M., Bohec M., Bougrier S., Lassus P., Truquet P. 1996, Feeding responses of Crassostrea gigas (Thunberg) to inclusion of different proportions of toxic dinoflagellates in their diet. Oceanol. Acta 19, 177-182.
Bauder, A.G., Cembella, A.D., 2000, Viability of the toxic dinoflagellate Prorocentrum lima following ingestion and gut passage in the bay scallop Argopecten irradians. J. Shellfish Res. 19, 321-324.
Béchemin, C. Grzebyk, D., Hachame, F., Hummert, C., Maestrini, S.Y., 1999, Effect of different nitrogen/phosphorus nutrient ratios on the toxin content in Alexandrium minutum (AM89BM). Aquat. Microb. Ecol. 20, 157-165. CrossRef
Bricelj, V.M., Greene, M., Cembella, A.D., 1993, Growth of the blue mussel Mytilus edulis on toxic Alexandrium fundyense and effects of gut passage on dinoflagellate cells. Toxic phytoplankton blooms in the sea. Dev. Mar. Biol. 3, 371-376.
Carriker, M.R., 1992, Introductions and transfers of molluscs: risk considerations and implications. J. Shellfish Res. 11, 507-510.
Coughlan, J., 1969, The estimation of filtering rate from the clearance of suspensions. Mar. Biol. 2, 356-358. CrossRef
Garcés, E., Masó, M., Camp, J., 2002, Role of temporary cysts in the population dynamics of Alexandrium taylori (Dinophyceae). J. Plankton Res. 24, 681-686. CrossRef
Guillard R.R.L., 1973, Division rates. In: Stein J.R. (Eds.) Handbook of phycological methods: culture methods and growth measurements. Cambridge University Press, pp. 289-311.
Hallegraeff, G.M., 1993, A review of harmful algal blooms and their apparent global increase. Phycologia 32, 79-99. CrossRef
Harrison, P.J., Waters, R., Taylor, F.J.R., 1980, A broad spectrum artificial seawater medium for coastal and open ocean phytoplankton. J. Phycol. 16, 28-35. CrossRef
Imada N., Honjo H., Shibata Y., Oshima K., Nagai Y., Matsuyama Uchida T., 2001, The quantities of Heterocapsa circularisquama cells transferred with shellfish consignments and the possibility of its establishment in new areas. In: Hallegraeff G.M, Blackburn S.L., Bolch C.J., Lewis R.J. (Eds.) Harmful Algal Blooms 2000, IOC-Unesco, pp. 474-476.
Laabir, M., Gentien, P., 1999, Survival of toxic dinoflagellates after gut passage in the pacific oyster Crassostrea gigas Thunberg. J. Shellfish Res. 18, 217-222.
Lassus, P., Baron, R., Garen, P., Truquet, P., Masselin, P., Bardouil, M., Leguay, D., Amzil, Z., 2004, Paralytic shellfish poison outbreaks in the Penzé estuary: Environmental factors affecting toxin uptake in the oyster, Crassostrea gigas. Aquat. Living Resour. 17, 207-214. CrossRef
Lilly, E.L., Kulis, D.M., Gentien, P., Anderson, D.M., 2002, Paralytic shellfish poisoning toxins in France linked to a human-introduced strain of Alexandrium catenella from the western Pacific: evidence from DNA and toxin analysis. J. Plankton Res. 24, 443-452. CrossRef
Marasovic, I., 1993, Preliminary observations on the relationship between temporary and resting cysts of Lingulodinium polyedra (Stein) Dodge. Toxic phytoplankton blooms in the sea. Dev. Mar Biol. 3, 139-142.
Masselin P., Amzil Z., Abadie E., Carreras A., Chiantella C., Le Bec C., Nézan E., Truquet P., 2001, Paralytic shellfish poisoning on the French Mediterranean coast in autumn 1998 : Alexandrium tamarense complex (Dinophyceae) as causative agent. In: Hallegraeff G.M., Blackburn S.I., Bolch C.J., Lewis R.J. (Eds.) Harmful Algal Blooms 2000, IOC-Unesco, pp 26-29.
Negri A.P., Bolch C.J.S., Blackburn S.I., Disckman M., Lleweuyn L.E., Mendez S., 2001, Paralytic shellfish toxins in Gymnodinium catenatum strains from six countries. In: Hallegraeff G.M., Blackburn S.I., Bolch C.J., Lewis R.J. (Eds.) Harmful Algal Blooms 2000, IOC-Unesco, pp. 210-213.
Oshima, Y., 1995, Postcolumn derivatization liquid chromatographic method for paralytic shellfish toxins. J. AOAC International 78, 528-532.
Persson, A., Smith, B.C., Wikfors, G.H., Quilliam, M., 2006, Grazing on toxic Alexandrium fundyense resting cysts and vegetative cells by the eastern oyster (Crassostrea virginica). Harmful Algae 5, 678-684. CrossRef
Scarratt, A.M., Scarratt, D.J., Scarratt, M.G., 1993, Survival of live Alexandrium tamarense cells in mussel and scallop spat under simulated transfer conditions. J. Shellfish Res. 12, 383-388.
Shumway, J.J., Burkhoder, J.A.M., Glasgow, H.B., 2002, Interactions between the toxic estuarine Pfiesteria piscicida and two species of bivalve molluscs. Mar. Ecol Prog. Ser. 245, 1-10
Shumway, S.E., 1990, A review of the effects of algal blooms on shellfish and aquaculture. J. World Aquac. Soc. 21, 65-104. CrossRef
Shumway, S.E., Cucci, T.L., 1987, The effects of the toxic dinoflagellate Protogonyaulax tamarensis on the feeding and behaviour of bivalve molluscs. Aquat. Toxicol. 10, 9-27. CrossRef
Siu, G.K.Y., Young, M.L.C., Chan, D.K.O., 1997, Environmental and nutritional factors which regulate population dynamics and toxin production in the dinoflagellate Alexandrium catenella. Hydrobiologia 352, 117-140. CrossRef
Sullivan, J.M., Swift, E., Donaghay, P.L., Rines, J.E.B., 2003, Small-scale turbulence affects the division rate and morphology of two red-tide dinoflagellates. Harmful Algae 2, 183-199. CrossRef
Van den Bergh, J.C.J., Numes, P.A.L., Dotinga, H.M., Kooistra, W.H.C., Vrieling, E.G., Peperzak, L., 2002, Exotic harmful algae in marine ecosystems: an integrated biological-economic-legal analysis of impacts and policies. Mar. Policy 26, 59-74. CrossRef