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Nitrite released in haemocytes from Mytilus galloprovincialis, Crassostrea gigas and Ruditapes decussatus upon stimulation with phorbol myristate acetate

Published online by Cambridge University Press:  15 May 2000

Mùnusamy Arumugan ,
Bernard Romestand  and
Jean Torreilles
Mùnusamy Arumugan
Affiliation:
Department of Zoology, Life Sciences Building, Guindy Campus, Chennai, 600 025 India UMR 219, CP 80, Université Montpellier II, 2 place E. Bataillon, 34095 Montpellier cedex 5, France
Bernard Romestand
Affiliation:
Department of Zoology, Life Sciences Building, Guindy Campus, Chennai, 600 025 India UMR 219, CP 80, Université Montpellier II, 2 place E. Bataillon, 34095 Montpellier cedex 5, France
Jean Torreilles
Affiliation:
UMR 219, CP 80, Université Montpellier II, 2 place E. Bataillon, 34095 Montpellier cedex 5, France
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Abstract

In order to demonstrate the involvement of nitric oxide in the defence systems of marine bivalves, we investigated the production of superoxide and nitrite, following in vitro phorbol myristate acetate stimulation of Mytilus galloprovincialis, Crassostrea gigas and Ruditapes decussatus haemocytes. Whereas M. galloprovincialis and C. gigas haemocytes were found to produce superoxide and nitrite, R. decussatus haemocytes were found to be unable to generate either of these mediators. Nitrite is a stable end product of nitric oxide and peroxynitrite as well; it appeared therefore that some marine bivalves, to kill microbial pathogens, use NADPH-oxidase and nitric oxide-synthase pathways. This was confirmed at an experimental level where inhibitors of both enzymatic pathways blocked the production of nitrite. Moreover, this notion was strengthened by the inability of the haemocytes from R. decussatus, which cannot produce superoxide, to release nitrite when stimulated.

Keywords

haemocytesimmune defencebivalvenitritereactive oxygen intermediates
Type
Research Article
Information
Aquatic Living Resources , Volume 13 , Issue 3 , May 2000 , pp. 173 - 177
Copyright
© Elsevier, Inra, Ifremer, Cemagref, Ird, Cnrs, 2000

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