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Characteristics of stripped and testicular Northern pike (Esox lucius) sperm: spermatozoa motility and velocity

Published online by Cambridge University Press:  29 April 2008

Martin Hulak ,
Marek Rodina  and
Otomar Linhart
Martin Hulak
Affiliation:
University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 389 25 Vodnany, Czech Republic
Marek Rodina
Affiliation:
University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 389 25 Vodnany, Czech Republic
Otomar Linhart
Affiliation:
University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 389 25 Vodnany, Czech Republic
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Abstract

The effects of osmolality and accidental urine contamination on spermatozoa velocity and motility were studied, combined with an examination of the biological characteristics of stripped and testicular sperm. Analysis of Northern pike sperm showed higher ionic concentrations of Na+ (123 ± 9 mM), Cl- (127 ± 7 mM), and K+ (35 ± 5 mM) in the seminal fluid of testicular sperm (TS), than in that of stripped sperm (SS): Na+ (116 ± 9 mM), Cl− (116 ± 7 mM) and K+ (25 ± 4 mM). Highest osmolality of seminal fluid was observed in TS with a value of 358 ± 77 mOsmol kg−1 compared with 273 ± 21 mOsmol kg−1 for SS and 68 ± 36 mOsmol kg−1 for urine. A significantly higher spermatozoa concentration was observed in TS (34 ± 5 × 109 ml−1) than in SS (23 ± 4 × 109 ml−1). Spermatozoa concentration per male and per kg body weight was 22 ± 17 × 109 for TS and 18 ± 2 × 109 for SS, respectively. Both TS and SS showed significantly higher spermatozoa velocities and motilities after dilution in urine than after dillusion in distilled water during the activity period. In conclusion, the results obtained from the present study provide information on northern pike sperm physiology that be used to improve sperm management efficiency for this species.

Keywords

AquacultureSeminal plasmaGamete managementSperm physiologyArtificial reproduction
Type
Research Article
Information
Aquatic Living Resources , Volume 21 , Issue 2 , April 2008 , pp. 207 - 212
Copyright
© EDP Sciences, IFREMER, IRD, 2008

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References

Alavi, S.M.H., Cosson, J., 2006, Sperm motility in fishes. (II) Effects of ions and osmolality: A review. Cell. Biol. Int. 30, 1–14. CrossRef
Billard, R., Debruille, M., Gérard, J.P., Montalembert, G., 1976, L'insémination artificielle du brochet. Bull. Fr. Piscic. 262, 30–34. CrossRef
Billard, R., 1978, Changes in structure and fertilizing ability of marine and freshwater fish spermatozoa diluted in media of various salinities. Aquaculture 14, 187–198. CrossRef
Billard, R., Marcel, J., 1980, Stimulation of spermiation and induction of ovulation in pike (Esox lucius). Aquaculture 21, 181–195. CrossRef
Cacot, P., Eeckhoutte, P., Muon, D.T., Trieu, N.V., Legendre, M., Mariojouls, C., Lazard, J., 2003, Induced spermiation and milt management in Pangasius bocourti (Sauvage, 1880). Aquaculture 215, 67–77. CrossRef
Caille, N., Rodina, M., Kocour, M., Gela, D., Flajšhans, M., Linhart, O., 2006, Quantity, motility and fertility of tench Tinca tinca (L.) sperm in relation to LHRH analogue and carp pituitary treatments. Aquac. Int. 14, 75–87. CrossRef
Chauvaud, L., Cosson, J., Suquet, M., Billard, R., 1995, Sperm motility in turbot, (Scophthalmus maximus) - initiation of movement and changes with time of swimming characteristics. Environ. Biol. Fishes 43, 341–349. CrossRef
Cosson, J., Linhart, O., Mims, S.D., Shelton, W.L., Rodina, M., 2000, Analysis of motility parameters from paddlefish and shovelnose sturgeon spermatozoa. J. Fish Biol. 56, 1348–1367. CrossRef
Cosson, J., 2004, The ionic and osmotic factors controlling motility of fish spermatozoa. Aquac. Int. 12, 69–85. CrossRef
Dietrich, G.J., Kowalski, R., Wojtczak, M., 2005, Motility parameters of rainbow trout (Oncorhynchus mykiss) spermatozoa in relation to sequential collection of milt, time of post-mortem storage and anesthesia. Fish Physiol. Biochem. 31, 1–9. CrossRef
Dreanno, C., Suquet, M., Desbruyeres, E., 1998, Effect of urine on semen quality in turbot (Psetta maxima). Aquaculture 169, 247-262. CrossRef
Ginsburk A.S., 1968, Fertilization in fishes and the problem of polyspermy. Moskva, Izdatelnaya Nauka.
Glogowski, J., Kwasnik, M., Piros, B., Dabrowski, K., Goryczko, K., Dobosz, S., Kuzminski, H., Ciereszko, A., 2000, Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success. Aquac. Res. 31, 289–296. CrossRef
FAO Fishery Statistics, 2006, available at http://www.fao.org/fi/statist/statist.asp.
Koldras, M., Moczarski, M., 1983, Properties of pike Esox lucius L. milt and its cryopreservation. Pol. Arch. Hydrobiol. 30, 69–78.
Kouril, J., Hamackova, J., 1975, Fecundity of pond-reared Pike (Esox lucius L). Czech J. Anim. Sci. 20, 841–849.
Lahnsteiner, F., Berger, B., Weismann, T., Patzner, R., 1995, Fine-structure and motility of spermatozoa and composition of the seminal plasma in the perch. J. Fish Biol. 47, 492–508. CrossRef
Lahnsteiner, F., Berger, B., Weismann, T., Patzner, R.A., 1997, Sperm structure and motility of the freshwater teleost Cottus gobio. J. Fish Biol. 50, 564–574.
Lahnsteiner, F., Weismann, T., Partner, R.A., 1998, An efficient method for cryopreservation of testicular sperm from the northern pike, Esox lucius L. Aquac. Res. 29, 341–347. CrossRef
Lin F., Liu L., Dabrowski K., 1996, Characteristics of muskellunge spermatozoa .1. Ultrastructure of spermatozoa and biochemical composition of semen. Trans. Am. Fish. Soc. 125 187–194.
Linhart, O., 1984, Evaluation of pike and European catfish sperm. Bull. VURH Vodnany 20, 22–33.
Linhart, O., Kouril, J., Hamáèkova, J., 1987, Increased rate of egg fertilization in artificial propagation of European catfish (Silurus glanis) by means of suppressing the movements of spermatozoa with immobilization solution. Aquaculture 65, 353–358. CrossRef
Linhart, O., Benesovsky, J., 1991, Artificial insemination in asp (Aspius aspius L.). Ziv.Vyr. 36, 973–980.
Linhart O., Proteau J.P., Redondo C., Billard R., 1991, Preservation of gametes in European catfish (Silurus glanis L.). 4th Int. Symp. Reproductive Physiology of fish, 7–12 July, 1991, Norwich, 283.
Linhart, O., Mims, S.D., Shelton, W.L., 1995, Motility of spermatozoa from Shovelnose sturgeon and Paddlefish. J. Fish Biol. 47, 902–909. CrossRef
Linhart O., Walford J., Sivaloganathan B., Lam T.J., 1999, Effects of osmolality and ions on the motility of stripped and testicular sperm of freshwater- and seawater-acclimated tilapia, Oreochromis mossambicus. J. Fish Biol.55, 1344–1358.
Linhart O., Cosson J., Mims S.D., Shelton W.L., Rodina M., 2002, Effects of ions on the motility of fresh and demembranated paddlefish (Polyodon spathula) spermatozoa Reproduction 124, 713–719.
Linhart, O., Mims, S.D., Gomelsky, B., Hiott, A.E., Shelton, W.L., Cosson, J., Rodina, M., Gela, D., Bastl, J., 2003, Ionic composition and osmolality of paddlefish Polyodon spathula, Acipenseriformes) seminal fluid. Aquac. Int. 11, 357–368. CrossRef
Linhart, O., Rodina, M., Bastl, J., Cosson, J., 2003, Urinary bladder, ionic composition of seminal fluid and urine with characterization of sperm motility in tench (Tinca tinca L.). J. Appl. Ichthyol. 19, 177–181. CrossRef
Linhart, O., Rodina, M., Gela, D., Kocour, M., 2004, Optimization of artificial propagation in European catfish, Silurus glanis L. Aquaculture 235, 619–632. CrossRef
ontalembert Jalabert, G. Bry, B. C., 1978a, Precocious induction of maturation and ovulation in northern pike (Esox lucius). Ann. Biol. Anim. Biochem. Biophys. 18, 969–975.
Montalembert, G., Bry, C., Billard, R., 1978b, Control of Reproduction in the Northern Pike. American Fisheries Society, Spec. Publ. 11, 217–225.
Montalembert, G., Marcel, J., Billard, R., 1980, La spermiation chez le brochet. 1) Evolution de la quantite de sperme recolte au cours de la saison de reproduction. Bull. Fr. Piscic. 276, 85–103.
Perchec, G., Cosson, J., Andre, F., Billard, R., 1995, Degradation of the quality of carp sperm by urine contamination during stripping. Aquaculture 129, 135–136. CrossRef
Poupard, G.P., Paxion, C., Cosson, J., Jeulin, C., Fierville, F., Billard, R., 1998, Initiation of carp spermatozoa motility and early ATP reduction after milt contamination by urine. Aquaculture 160, 317–328. CrossRef
Rodina, M., Cosson, J., Gela, D., Linhart, O., 2004, Kurokura solution as immobilizing medium for spermatozoa of tench (Tinca tinca L.). Aquac. Int 12, 119–131. CrossRef
Steinberg D., 1992, Northern Pike and Muskie. CY DeCrosse Inc.