Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-29T03:19:32.863Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic variability in the tolerance of natural populations of Simocephalus vetulus (Müller, 1776) to lethal levels of sodium chloride

Published online by Cambridge University Press:  14 March 2012

Cláudia Loureiro ,
Bruno B. Castro ,
Maria Teresa Claro ,
Artur Alves ,
M. Arminda Pedrosa  and
Fernando Gonçalves
Cláudia Loureiro
Affiliation:
Department of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal
Bruno B. Castro*
Affiliation:
Department of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal
Maria Teresa Claro
Affiliation:
Department of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal
Artur Alves
Affiliation:
Department of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal
M. Arminda Pedrosa
Affiliation:
Unidade de I&D No. 70/94 – Química-Física Molecular/FCT, MCT, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, Portugal
Fernando Gonçalves
Affiliation:
Department of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal
*
*Corresponding author: [email protected]
Get access

Abstract

Using several clonal lineages of Simocephalus vetulus (Cladocera, Daphniidae) as a random sample, we investigated the genetic component of the halotolerance of one brackish and two freshwater populations of this littoral filter feeder. We hypothesized that genotypes from the brackish population were more tolerant than freshwater ones, via adaptation to local environmental conditions. Clonal identity was established by a cost-effective molecular fingerprinting technique (microsatellite-primed polymerase chain reaction (MSP-PCR)). Two distinct methodologies were used to assess cladoceran sensitivity to synthetic-grade sodium chloride (NaCl): (i) standard 48-h acute assays and (ii) 12-h survival time (ST) trials. No correlation was found between acute EC50 and ST values. The sensitivity of brackish and freshwater clones was comparable in terms of acute EC50 (varied from 2.28 to 3.83 g.L−1). On the contrary, genetically determined differential tolerance to NaCl among populations was found for ST: all brackish genotypes, except one, were more resilient (ST>120 min) than freshwater clones (ST<120 min). Bearing in mind that these results were obtained with isolates from the extant population, it is surprising that the range of acute sensitivity of the freshwater and brackish genotypes was similar, and that the only difference between them was the ability of brackish clones to survive longer under high salinity stress (6 g.L−1, in ST trials). We must conclude that the effect of salinity (original environment context) on the selection of genotypes was weaker than we had expected and than other authors have shown for other stressors.

Keywords

Genetic variabilitygenotype–environment interactionsodium chloridehalotoleranceSimocephalus vetulus
Type
Research Article
Information
Annales de Limnologie - International Journal of Limnology , Volume 48 , Issue 1 , 2012 , pp. 95 - 103
Copyright
© EDP Sciences, 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abbot, P., 2001. Individual and population variation in invertebrates revealed by inter-simple sequence repeats (ISSRs). J. Insect Sci., 1.8, 3.Google Scholar
Aladin, N.V. and Potts, W.T.W., 1995. Osmoregulatory capacity of the Cladocera. J. Comp. Physiol. B., 164, 671–683.CrossRefGoogle Scholar
Alonso, M., 1996. Fauna Ibérica. Crustacea, Branquiopoda, vol 7. Museo Nacional de Ciencias Naturales. Consejo Superior de Investigaciones Científicas, Madrid, 486 p.Google Scholar
Alves, A., Phillips, A., Henriques, I. and Correia, A., 2007. Rapid differentiation of species of Botryosphaeriaceae by PCR fingerprinting. Res. Microbiol., 158, 112–121.CrossRefGoogle ScholarPubMed
Antunes, S.C., Castro, B.B. and Goncalves, F., 2004. Effect of food level on the acute and chronic responses of daphnids to lindane. Environ. Pollut., 127, 367–375.CrossRefGoogle ScholarPubMed
Arnér, M. and Koivisto, S., 1993. Effects of salinity on metabolism and life history characteristics of Daphnia magna. Hydrobiologia, 259, 69–77.CrossRefGoogle Scholar
ASTM, 1980. Standard Practice for Conducting Acute Toxicity Tests with Fishes, Macroinvertebrates and Amphibians. American Society for Testing and Materials, Philadelphia.
Baird, D.J., Soares, A.M.V.M., Girling, A., Barber, I., Bradley, M.C. and Calow, P., 1989. The long-term maintenance of Daphnia magna Straus for use in ecotoxicity tests: problems and prospects. In: Lokke, H., Tyle, H. and Bro-Rasmussen, F. (eds.), Proceedings of the First European Conference on Ecotoxicology, Lyngby, 144–148.Google Scholar
Barry, M.J., Tibby, J., Tsitsilas, A., Mason, B., Kershaw, P. and Heijnis, H., 2005. A long term lake-salinity record and its relationships to Daphnia populations. Arch. Hydrobiol., 163, 1–23.CrossRefGoogle Scholar
Boersma, M., De Meester, L. and Spaak, P., 1999. Environmental stress and local adaptation in Daphnia magna. Limnol. Oceanogr., 44, 393–402.Google Scholar
Brede, N., Thielsch, A., Sandrock, C., Spaak, P., Keller, B., Streit, B. and Schwenk, K., 2006. Microsatellite markers for European Daphnia. Mol. Ecol. Notes, 6, 536–539.CrossRefGoogle Scholar
Chopelet, J., Blier, P.U. and Dufresne, F., 2008. Plasticity of growth rate and metabolism in Daphnia magna populations from different thermal habitats. J. Exp. Zool. A: Ecol. Genet. Physiol., 309A, 553–562.CrossRefGoogle Scholar
Cousyn, C., De Meester, L., Colbourne, J.K., Brendonck, L., Verschuren, D. and Volckaert, F., 2001. Rapid, local adaptation of zooplankton behavior to changes in predation pressure in the absence of neutral genetic changes. Proc. Natl. Acad. Sci. USA, 98, 6256–6260.CrossRefGoogle ScholarPubMed
Culley, T.M. (2005) Population Genetic Analysis of ISSR Data. Available online http://www.artsci.uc.edu/collegedepts/biology/fac_staff/culley/docs/Population%20Genetic%20Analysis%20of%20ISSR%20Data.pdf (accessed September 2011).
De Meester, L., 1996a. Evolutionary potential and local genetic differentiation in a phenotypically plastic trait of a cyclical parthenogen, Daphnia magna. Evolution, 50, 1293–1298.CrossRefGoogle Scholar
De Meester, L., 1996b. Local genetic differentiation and adaptation in freshwater zooplankton populations: patterns and processes. Ecoscience, 3, 385–399.CrossRefGoogle Scholar
De Meester, L., Gómez, A., Okamura, B. and Schwenk, K., 2002. The monopolization hypothesis and the dispersal-gene flow paradox in aquatic organisms. Acta Oecol. - Int. J. Ecol., 23, 121–135.CrossRefGoogle Scholar
De Meester, L., Vanoverbeke, J., De Gelas, K., Ortells, R. and Spaak, P., 2006. Genetic structure of cyclic parthenogenetic zooplankton populations – a conceptual framework. Arch. Hydrobiol., 167, 217–244.CrossRefGoogle Scholar
Dusinský, R., Kúdela, M., Stloukalová, V. and Jedlicka, L., 2006. Use of inter-simple sequence repeat (ISSR) markers for discrimination between and within species of blackflies (Diptera: Simuliidae). Biologia, Bratislava, 61, 299–304.CrossRefGoogle Scholar
Elendt, B.P. and Bias, W.R., 1990. Trace nutrient deficiency in Daphnia magna cultured in standard medium for toxicity testing. Effects of the optimization of culture conditions on life history parameters of D. magna. Water Res., 24, 1157–1167.CrossRefGoogle Scholar
Gonçalves, A.M.M., Castro, B.B., Pardal, M.A. and Gonçalves, F., 2007. Salinity effects on survival and life history of two freshwater cladocerans (Daphnia magna and Daphnia longispina). Ann. Limnol. - Int. J. Limnol., 43, 13–20.CrossRefGoogle Scholar
Grasela, J.J. and McIntosh, A.H., 2003. Application of inter-simple sequence repeats to insect cell lines: identification at the clonal and tissue-specific level. Vitro Cell. Dev. Biol. Animal, 39, 356–363.2.0.CO;2>CrossRefGoogle ScholarPubMed
Hann, B.J., 1995. Genetic-variation in Simocephalus (Anomopoda, Daphniidae) in North-America – patterns and consequences. Hydrobiologia, 307, 9–14.CrossRefGoogle Scholar
Hoffmann, A.A. and Parsons, P.A., 1993. Evolutionary Genetics and Environmental Stress. Oxford University Press, New York, 296 p.Google Scholar
IPCC, 2008. Climate Change and Water – Technical Paper of the Intergovernmental Panel on Climate Change. IPCC Secretariat, Geneva.
ISO, 1996. Water Quality: Determination of the Inhibition of the Mobility of Daphnia Magna Straus (Cladocera, Crustacea) – Acute Toxicity Test. Internacional Organization for Standardization, Geneva.
Jeppesen, E., Sondergaard, M., Kanstrup, E., Petersen, B., Eriksen, R.B., Hammershoj, M., Mortensen, E., Jensen, J.P. and Have, A., 1994. Does the impact of nutrients on the biological structure and function of brackish and freshwater lakes differ? Hydrobiologia, 276, 15–30.CrossRefGoogle Scholar
Jeppesen, E., Sondergaard, M., Pedersen, A.R., Jurgens, K., Strzelczak, A., Lauridsen, T.L. and Johansson, L.S., 2007. Salinity induced regime shift in shallow brackish lagoons. Ecosystems, 10, 47–57.CrossRefGoogle Scholar
Karp, A. and Edwards, K.J., 1997. DNA markers: a global overview. In: Caetano-Anollés, G. and Gresshoff, P.M. (eds.), DNA Markers: Protocols, Applications, and Overviews. Wiley-Liss, New York, 1–13.Google Scholar
Lopes, I., Baird, D.J. and Ribeiro, R., 2004. Genetic determination of tolerance to lethal and sublethal copper concentrations in field populations of Daphnia longispina. Arch. Environ. Contam. Toxicol., 46, 43–51.CrossRefGoogle ScholarPubMed
Lopes, I., Baird, D.J. and Ribeiro, R., 2005. Genetically determined resistance to lethal levels of copper by Daphnia longispina: association with sublethal response and multiple/coresistance. Environ. Toxicol. Chem., 24, 1414–1419.CrossRefGoogle ScholarPubMed
Marques, S.C., Azeiteiro, U.M., Marques, J.C., Neto, J.M. and Pardal, M.A., 2006. Zooplankton and ichthyoplankton communities in a temperate estuary: spatial and temporal patterns. J. Plankton Res., 28, 297–312.CrossRefGoogle Scholar
Martínez-Jerónimo, F. and Martínez-Jerónimo, L., 2007. Chronic effect of NaCl salinity on a freshwater strain of Daphnia magna Straus (Crustacea: Cladocera): a demographic study. Ecotoxicol. Environ. Saf., 67, 411–416.CrossRefGoogle ScholarPubMed
Muyssen, B.T.A., Bossuyt, B.T.A. and Janssen, C.R., 2005. Inter- and intra-species variation in acute zinc tolerance of field-collected cladoceran populations. Chemosphere, 61, 1159–1167.CrossRefGoogle ScholarPubMed
Nielsen, D.L., Brock, M.A., Vogel, M. and Petrie, R., 2008. From fresh to saline: a comparison of zooplankton and plant communities developing under a gradient of salinity with communities developing under constant salinity levels. Mar. Freshw. Res., 59, 549–559.CrossRefGoogle Scholar
Northcote, T.G. and Hall, K.J., 2010. Salinity regulation of zooplanktonic abundance and vertical distribution in two saline meromictic lakes in south central British Columbia. Hydrobiologia, 638, 121–136.CrossRefGoogle Scholar
OECD, 2004. Daphnia sp., Acute Immobilization Test. Organization for the Economic Cooperation and Development, Paris.
Pfrender, M.E. and Lynch, M., 2000. Quantitative genetic variation in Daphnia: temporal changes in genetic architecture. Evolution, 54, 1502–1509.CrossRefGoogle ScholarPubMed
Ribeiro, R., Lopes, I., Pereira, A.M.M., Goncalves, F. and Soares, A.M.V.M., 2000. Survival time of Ceriodaphnia dubia in acid waters with metal contamination. Bull. Environ. Contam. Toxicol., 64, 130–136.CrossRefGoogle ScholarPubMed
Santangelo, J.M., Bozelli, R.L., Rocha, A.D. and Esteves, F.D., 2008. Effects of slight salinity increases on Moina micrura (Cladocera) populations: field and laboratory observations. Mar. Freshw. Res., 59, 808–816.CrossRefGoogle Scholar
Sarma, S.S.S., Nandini, S., Morales-Ventura, J., Delgado-Martínez, I. and González-Valverde, L., 2006. Effects of NaCl salinity on the population dynamics of freshwater zooplankton (rotifers and cladocerans). Aquat. Ecol., 40, 349–360.CrossRefGoogle Scholar
Schallenberg, M., Hall, C.J. and Burns, C.W., 2003. Consequences of climate-induced salinity increases on zooplankton abundance and diversity in coastal lakes. Mar. Ecol. – Prog. Ser., 251, 181–189.CrossRefGoogle Scholar
Schwenk, K., Sand, A., Boersma, M., Brehm, M., Mader, E., Offerhaus, D. and Spaak, P., 1998. Genetic markers, genealogies and biogeographic patterns in the cladocera. Aquat. Ecol., 32, 37–51.CrossRefGoogle Scholar
Teschner, M., 1995. Effects of salinity on the life-history and fitness of Daphnia magna – variability within and between populations. Hydrobiologia, 307, 33–41.CrossRefGoogle Scholar
USEPA, 2002. Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms (5th edn), US Environmental Protection Agency, Washington.
Weber, A. and Declerck, S., 1997. Phenotypic plasticity of Daphnia life history traits in response to predator kairomones: genetic variability and evolutionary potential. Hydrobiologia, 360, 89–99.CrossRefGoogle Scholar
Weider, L.J. and Hebert, P.D.N., 1987. Ecological and physiological differentiation among low-arctic clones of Daphnia pulex. Ecology, 68, 188–198.CrossRefGoogle Scholar
Wilson, R.W. and Taylor, E.W., 1993. The physiological responses of freshwater rainbow trout, Oncorhynchus mykiss, during acutely lethal copper. J. Comp. Physiol. B., 163, 38–47.CrossRefGoogle Scholar
Zietkiewicz, E., Rafalski, A. and Labuda, D., 1994. Genome fingerprinting by simples sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20, 176–183.CrossRefGoogle Scholar