Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-28T10:27:15.089Z Has data issue: false hasContentIssue false
  • English
  • Français

Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm

Published online by Cambridge University Press:  22 November 2012

T. F. Collares ,
V. F. Campos ,
G. Urtiaga ,
P. M. M. Leon ,
M. G. Amaral ,
C. P. Hartleben ,
A. J. McBride ,
O. A. Dellagostin ,
J. C. Deschamps  and
F. K. Seixas
...Show all authors
T. F. Collares
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
V. F. Campos
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
G. Urtiaga
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
P. M. M. Leon
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
M. G. Amaral
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
C. P. Hartleben
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
A. J. McBride
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
O. A. Dellagostin
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
J. C. Deschamps
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
F. K. Seixas
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
T. Collares*
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
*
†E-mail: [email protected]
Get access

Abstract

Erythropoietin (EPO) gene therapy can be used for several purposes; however, its effects on reproductive performance are unknown. The aim of this study was to evaluate the toxicological effects of non-viral (EPO) gene transfer on sperm motility, viability, morphology and concentration. Rabbit EPO cDNA was cloned into a pTarget mammalian expression vector. Rabbits were administered with: (1) pTarget/EPO vector, (2) recombinant human EPO (rHuEpo) and (3) saline (control). Both pTarget/EPO and rHuEpo significantly increased (P < 0.05) hematocrit levels 1 week after injection and they remained significantly higher than the control for up to 5 weeks (P < 0.05), showing that both EPO treatments were effective in stimulating the production of red blood cells in rabbits. The EPO gene transfer or rHuEPO administration had no significant effect (P > 0.05) on sperm motility, vigor, viability, concentration or morphology in the testis.

Keywords

EPOplasmid DNAsperm characteristicsrHuEPO
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 5 , May 2013 , pp. 778 - 783
Copyright
Copyright © The Animal Consortium 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

These authors contributed equally for this study.

References

Azzazy, HM, Mansour, MM, Christenson, RH 2009. Gene doping: of mice and men. Clinical Biochemistry 42, 435–441.CrossRefGoogle ScholarPubMed
Campos, VF, de Leon, PM, Komninou, ER, Dellagostin, OA, Deschamps, JC, Seixas, FK, Collares, T 2011a. NanoSMGT: Transgene transmission into bovine embryos using halloysite clay nanotubes or nanopolymer to improve transfection efficiency. Theriogenology 76, 1552–1560.Google Scholar
Campos, VF, Komninou, ER, Urtiaga, G, de Leon, PM, Seixas, FK, Dellagostin, OA, Deschamps, JC, Collares, T 2011b. NanoSMGT: Transfection of exogenous DNA on sex-sorted bovine sperm using nanopolymer. Theriogenology 75, 1476–1481.Google Scholar
Foresta, C, Mioni, R, Bordon, P, Miotto, D, Montini, G, Varotto, A 1994. Erythropoietin stimulates testosterone production in man. Journal of Clinical Endocrinology and Metabolism 78, 753–756.Google Scholar
Friedmann, T 2010. How close are we to gene doping? Hastings Center Reports 40, 20–22.Google Scholar
Friedmann, T, Rabin, O, Frankel, MS 2010. Ethics. Gene doping and sport. Science 327, 647–648.CrossRefGoogle ScholarPubMed
Haisma, HJ, de, HO 2006. Gene doping. International Journal of Sports Medicine 27, 257–266.Google Scholar
Jelkmann, W 2003. Erythropoietin. Journal of Endocrinological Investigation 26, 832–837.Google Scholar
Kobayashi, T, Yanase, H, Iwanaga, T, Sasaki, R, Nagao, M 2002. Epididymis is a novel site of erythropoietin production in mouse reproductive organs. Biochemical and Biophysical Research Communications 296, 145–151.CrossRefGoogle ScholarPubMed
Köseoğlu, B, Yilmaz, E, Ceylan, K, Uzun, E, Bayram, I, Hizli, F 2009. The protective effect of erythropoietin infusion on testicular torsion/detorsion: an experimental study. International Urology and Nephrology 41, 85–91.CrossRefGoogle ScholarPubMed
Lacombe, C, Mayeux, P 1998. Biology of erythropoietin. Haematologica 83, 724–732.Google Scholar
Marklund, S, Sandberg, K, Andersson, L 1996. Forensic tracing of horse identities using urine samples and DNA markers. Animal Biotechnology 7, 145–153.Google Scholar
Maruyama, H, Ataka, K, Gejyo, F, Higuchi, N, Ito, Y, Hirahara, H, Imazeki, I, Hirata, M, Ichikawa, F, Neichi, T, Kikuchi, H, Sugawa, M, Miyazaki, J 2001. Long-term production of erythropoietin after electroporation-mediated transfer of plasmid DNA into the muscles of normal and uremic rats. Gene Therapy 8, 461–468.Google Scholar
McKanna, TA, Toriello, HV 2010. Gene doping: the hype and the harm. Pediatric Clinics of North America 57, 719–727.CrossRefGoogle ScholarPubMed
Sharp, NC 2010. The human genome and sport, including epigenetics, gene doping, and athleticogenomics. Endocrinology Metabolism Clinics of North America 39, 201–215, xi.Google Scholar
Temma, K, Shimoya, K, Hashimoto, K, Zhang, Q, Koyama, M, and Murata, Y 2004. Detection of erythropoietin in human seminal plasma. Fertility and Sterility 81 (Suppl 1), 798–801.Google Scholar
Tug, N, Altunkaynak, ME, Aktas, RG, Kilic, U, Yilmaz, B, Cam, C, Karateke, A 2010. Does erythropoietin affect motility of spermatozoa? Archives of Gynecology and Obstetrics 281, 933–938.CrossRefGoogle ScholarPubMed
Wells, DJ 2008. Gene doping: the hype and the reality. British Journal of Pharmacology 154, 623–631.Google Scholar
Wells, DJ 2009. Gene doping: possibilities and practicalities. Medicine and Sport Science 54, 166–175.Google Scholar
Yamamoto, Y, Sofikitis, N, and Miyagawa, I 1997. Effects of erythropoietin, bromocryptine and hydralazine on testicular function in rats with chronic renal failure. Andrologia 29, 141–144.CrossRefGoogle ScholarPubMed
Yamazaki, T, Kanzaki, M, Kamidono, S, Fujisawa, M 2004. Effect of erythropoietin on Leydig cell is associated with the activation of Stat5 pathway. Molecular and Cellular Endocrinology 213, 193–198.Google Scholar
Yasuda, Y, Fujita, Y, Musha, T, Tanaka, H, Shiokawa, S, Nakamatsu, K, Mori, S, Matsuo, T, Nakamura, Y 2001. Expression of erythropoietin in human female reproductive organs. Archivio Italiano di Anatomia e di Embriologia 106, 215–222.Google Scholar