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Effects of capric acid on rumen methanogenesis and biohydrogenation of linoleic and α-linolenic acid

Published online by Cambridge University Press:  01 June 2009

G. Goel ,
K. Arvidsson ,
B. Vlaeminck ,
G. Bruggeman ,
K. Deschepper  and
V. Fievez
G. Goel
Affiliation:
Laboratory for Animal Nutrition and Animal Product Quality (Lanupro), Faculty of Bioscience Engineering, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium
K. Arvidsson
Affiliation:
Department of Agricultural Research for Northern Sweden, Swedish University of Agricultural Sciences, SE-901 83 Umeå, Sweden
B. Vlaeminck
Affiliation:
Laboratory for Animal Nutrition and Animal Product Quality (Lanupro), Faculty of Bioscience Engineering, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium
G. Bruggeman
Affiliation:
Vitamex N.V., Booiebos, 9031 Drongen, Belgium
K. Deschepper
Affiliation:
Vitamex N.V., Booiebos, 9031 Drongen, Belgium
V. Fievez*
Affiliation:
Laboratory for Animal Nutrition and Animal Product Quality (Lanupro), Faculty of Bioscience Engineering, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium
*
E-mail: [email protected]
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Abstract

Capric acid (C10:0), a medium chain fatty acid, was evaluated for its anti-methanogenic activity and its potential to modify the rumen biohydrogenation of linoleic (C18:2n-6) and α-linolenic acids (C18:3n-3). A standard dairy concentrate (0.5 g), supplemented with sunflower oil (10 mg) and linseed oil (10 mg) and increasing doses of capric acid (0, 10, 20 and 30 mg), was incubated with mixed rumen contents and buffer (1 : 4 v/v) for 24 h. The methane inhibitory effect of capric acid was more pronounced at the highest (30 mg) dose compared to the medium (20 mg) (−85% v. −34%), whereas the lower dose (10 mg) did not reduce rumen methanogenesis. A 23% decrease in total short-chain fatty acid (SCFA) production was observed, accompanied by shifts towards increased butyrate at 20 mg and increased propionate at 30 mg of capric acid (P < 0.001). Capric acid linearly decreased the extent of biohydrogenation of C18:2n-6 and C18:3n-3, by up to 60% and 86%, respectively. This reduction was partially due to a lower extent of lipolysis when capric acid was supplemented. Capric acid at 20 and 30 mg completely inhibited the production of C18:0 (P < 0.001), resulting in an accumulation of biohydrogenation intermediates, mainly C18:1t10 + t11 and C18:2t11c15. In contrast to effects on rumen fermentation (methane production and proportions of SCFA), 30 mg of capric acid did not induce major changes in rumen biohydrogenation as compared to the medium (20 mg) dose. This study revealed the dual action of capric acid, being inhibitory to both methane production and biohydrogenation of C18:2n-6 and C18:3n-3.

Keywords

capric acidrumen fermentationmethanebiohydrogenation
Type
Full Paper
Information
animal , Volume 3 , Issue 6 , June 2009 , pp. 810 - 816
Copyright
Copyright © The Animal Consortium 2009

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References

Ajisaka, N, Mohammed, N, Hara, K, Mikuni, K, Hara, K, Hashimoto, H, Kumata, T, Kanda, S, Itabashi, H 2002. Effects of medium-chain fatty acid–cyclodextrin complexes on ruminal methane production in vitro. Animal Science Journal 73, 479–484.CrossRefGoogle Scholar
Blaxter, KL, Czerkawski, JW 1966. Modification of methane production of sheep by supplementation of its diet. Journal of the Science of Food and Agriculture 17, 417–420.CrossRefGoogle ScholarPubMed
Boeckaert, C, Vlaeminck, B, Mestdagh, J, Fievez, V 2007. In vitro examination of DHA-edible micro algae: 1. Effect on rumen lipolysis and biohydrogenation of linoleic and α-linolenic acids. Animal Feed Science and Technology 136, 63–79.CrossRefGoogle Scholar
Boeckaert, C, Vlaeminck, B, Dijkstra, J, Abdulsudi, IZ, Van Nespen, T, Van Straalen, W, Fievez, V 2008. Effect of dietary starch or micro algae supplementation on rumen fermentation and milk fatty acid composition of dairy cows. Journal of Dairy Science 91, 4714–4727.CrossRefGoogle ScholarPubMed
Chow, TT, Fievez, V, Raes, K, Demeyer, D, De Smet, S 2003. Lipolysis and biohydrogenation of linoleic and α-linolenic acid in vitro: comparison of linseed sources and grass. Proceedings of British Society of Animal Science, p. 169.CrossRefGoogle Scholar
Chow, TT, Fievez, V, Moloney, AP, Raes, K, Demeyer, D, De Smet, S 2004. Effect of fish oil on in vitro rumen lipolysis, apparent biohydrogenation of linoleic and α-linolenic acid and accumulation of biohydrogenation intermediates. Animal Feed Science and Technology 117, 1–12.CrossRefGoogle Scholar
Dierick, NA, Decuypere, JA, Molly, K, Van Beek, E, Vanderbeke, E 2002. The combined use of triacylglycerols (TAGs) containing medium-chain fatty acids (MCFAs) and exogenous lipolytic enzymes as an alternative to nutritional antibiotics in piglet nutrition. II. In vivo release of MCFAs in gastric cannulated and slaughtered piglets by endogenous and exogenous lipases; effects on the luminal gut flora and growth performance. Livestock Production Science 76, 1–16.CrossRefGoogle Scholar
Dohme, F, Machmüller, A, Wasserfallen, A, Kreuzer, M 2001. Ruminal methanogenesis as influenced by individual fatty acids supplemented to complete ruminant diets. Letters in Applied Microbiology 32, 47–51.CrossRefGoogle ScholarPubMed
Dohme, F, Fievez, V, Raes, K, Demeyer, D 2003. Increasing levels of two different fish oils lower ruminal biohydrogenation of eicosapentaenoic and docosahexaenoic acid in vitro. Animal Research 52, 309–320.CrossRefGoogle Scholar
Fievez, V, Dohme, F, Danneels, M, Raes, K, Demeyer, D 2003. Fish oils as potent rumen methane inhibitors and associated effects on rumen fermentation in vitro and in vivo. Animal Feed Science and Technology 104, 41–58.CrossRefGoogle Scholar
Fievez, V, Boeckaert, C, Vlaeminck, B, Mestdagh, J, Demeyer, D 2007. In vitro examination of DHA-edible micro algae: 2. Effect on rumen methane production and apparent degradability of hay. Animal Feed Science and Technology 136, 80–95.CrossRefGoogle Scholar
Folch, J, Lees, M, Stanley, SGH 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Freese, E, Sheu, CW, Galliers, E 1973. Function of lipophilic acids as antimicrobial food additives. Nature 241, 321–325.CrossRefGoogle ScholarPubMed
IPCC (Intergovernment Panel on Climate Change) 2001. Climate change 2001. The scientific basis. Cambridge University Press, Cambridge, UK.Google Scholar
Jenkins, TC, Wallace, RJ, Moate, PJ, Mosley, EE 2008. Recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. Journal of Animal Science 86, 397–412.CrossRefGoogle ScholarPubMed
Johnson, KA, Johnson, DE 1995. Methane emissions from cattle. Journal of Animal Science 73, 2483–2492.CrossRefGoogle ScholarPubMed
Macfarlane, S, Macfarlane, GT 2003. Regulation of short-chain fatty acid production. Proceedings of the Nutrition Society 62, 67–72.CrossRefGoogle ScholarPubMed
Machmüller, A, Ossowski, DA, Kreuzer, M 2000. Comparative evaluation of the effects of coconut oil, oilseeds and crystalline fat on methane release, digestion and energy balance in lambs. Animal Feed Science and Technology 85, 41–60.CrossRefGoogle Scholar
Matsumoto, M, Kobayashi, T, Takenaka, A, Itabashi, H 1991. Defaunation effects of medium-chain fatty acids and their derivatives on goat rumen protozoa. Journal of General and Applied Microbiology 37, 439–445.CrossRefGoogle Scholar
Newbold, CJ, Lassalas, B, Jouany, JP 1995. The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro. Letters in Applied Microbiology 21, 230–234.CrossRefGoogle ScholarPubMed
Panayakaew, P, Goel, G, Lourenço, M, Yuangklang, C, Fievez, V 2008. Medium-chain fatty acids from coconut oil or krabok oil to reduce in vitro rumen methanogenesis. Communications in Agricultural and Applied Biological Sciences 73, 189–192.Google Scholar
Raes, K, De Smet, S, Demeyer, D 2001. Effect of double-muscling in Belgian Blue young bulls on the intramuscular fatty acid composition with emphasis on conjugated linoleic acid and polyunsaturated fatty acids. Animal Science 73, 253–260.CrossRefGoogle Scholar
Soliva, CR, Hindrichsen, IK, Meile, L, Kreuzer, M, Machmüller, A 2004a. Effects of mixtures of lauric and myristic acid on rumen methanogens and methanogenesis in vitro. Letters in Applied Microbiology 37, 35–39.CrossRefGoogle Scholar
Soliva, CR, Meile, L, Hindrichsen, IK, Kreuzer, M, Machmüller, A 2004b. Myristic acid supports the immediate inhibitory effect of lauric acid on ruminal methanogens and methane release. Anaerobe 10, 269–276.CrossRefGoogle ScholarPubMed
Sutton, JD, Knight, R, McAllan, AB, Smith, RH 1983. Digestion and synthesis in the rumen of sheep given diets supplemented with free and protected oils. British Journal of Nutrition 49, 419–432.CrossRefGoogle ScholarPubMed
Van Nevel, CJ, Demeyer, DI 1996. Influence of pH on lipolysis and biohydrogenation of soybean oil by rumen contents in vitro. Reproduction Nutrition and Development 36, 53–63.CrossRefGoogle ScholarPubMed
Vlaeminck, B, Dufour, C, van Vuuren, AM, Cabrita, ARJ, Dewhurst, RJ, Demeyer, D, Fievez, V 2005. Use of odd and branched-chain fatty acids in rumen contents and milk as a potential microbial marker. Journal of Dairy Science 88, 1031–1042.CrossRefGoogle ScholarPubMed
Wolff, RL, Bayard, CC, Fabien, RJ 1995. Evaluation of sequential methods for the determination of butterfat fatty acid composition with emphasis on trans-18:1 acids. Application to the study of seasonal variations in French butters. Journal of the American Oil Chemists’ Society 72, 1471–1483.CrossRefGoogle Scholar