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Effect of extruded linseeds alone or in combination with fish oil on intake, milk production, plasma metabolite concentrations and milk fatty acid composition in lactating goats

Published online by Cambridge University Press:  10 December 2014

L. Bernard ,
C. Leroux ,
J. Rouel ,
C. Delavaud ,
K. J. Shingfield  and
Y. Chilliard
L. Bernard*
Affiliation:
INRA, UMR 1213 Herbivores, Equipe Alimentation Génomique Lactation, Site de Theix, F-63122 Saint-Genès-Champanelle, France Clermont Université, VetAgro Sup, UMR1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France
C. Leroux
Affiliation:
INRA, UMR 1213 Herbivores, Equipe Alimentation Génomique Lactation, Site de Theix, F-63122 Saint-Genès-Champanelle, France Clermont Université, VetAgro Sup, UMR1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France
J. Rouel
Affiliation:
INRA, UMR 1213 Herbivores, Equipe Alimentation Génomique Lactation, Site de Theix, F-63122 Saint-Genès-Champanelle, France Clermont Université, VetAgro Sup, UMR1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France
C. Delavaud
Affiliation:
INRA, UMR 1213 Herbivores, Equipe Alimentation Génomique Lactation, Site de Theix, F-63122 Saint-Genès-Champanelle, France Clermont Université, VetAgro Sup, UMR1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France
K. J. Shingfield
Affiliation:
MTT Agrifood Research Finland, Animal Production Research, FI-31600 Jokioinen, Finland Institute of Biological, Rural and Environmental Sciences, Aberystwyth University, Gogerddan, SY23 3EE, UK
Y. Chilliard
Affiliation:
INRA, UMR 1213 Herbivores, Equipe Alimentation Génomique Lactation, Site de Theix, F-63122 Saint-Genès-Champanelle, France Clermont Université, VetAgro Sup, UMR1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France
*
*E-mail: [email protected]
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Abstract

Based on the potential benefits for long-term human health, there is interest in developing sustainable nutritional strategies for lowering medium-chain saturated fatty acids (FA) and increasing specific unsaturated FA in ruminant milk. Dietary supplements of extruded linseeds (EL), fish oil (FO) or a mixture of EL and FO increase cis-9,trans-11 CLA and long-chain n-3 polyunsaturated FA in bovine milk. Supplements of FO cause milk fat depression in lactating cows, but information for dairy goats is limited. A total of 14 Alpine goats were used in a replicated 3×3 Latin square with 28-days experimental periods to examine the effects of EL alone or in combination with FO on animal performance, milk fat synthesis and milk FA composition. Treatments comprised diets based on natural grassland hay supplemented with no additional oil (control), 530 of EL or 340 g/day of EL and 39 g/day of FO (ELFO). Compared with the control, ELFO tended (P=0.08) to lower milk fat yield, whereas EL increased (P<0.01) milk fat content and yield (15% and 10%, respectively). Relative to EL, ELFO decreased (P<0.01) milk fat content and yield (19% and 17%, respectively). Relative to the control and ELFO, EL decreased (P<0.05) milk 10:0 to 16:0 and odd- and branched-chain FA content and increased 18:0, cis-18:1, trans-13 18:1 (and their corresponding ∆-9 (desaturase products), trans-12,cis-14 CLA, cis-13,trans-15 CLA, cis-12,trans-14 CLA and trans-11,cis-13 CLA and 18:3n-3 concentrations. ELFO was more effective for enriching (P<0.05) milk cis-9, trans-11 CLA and trans-11 18:1 concentrations (up to 5.4- and 7.1-fold compared with the control) than EL (up to 1.7- and 2.5-fold increases). Furthermore, ELFO resulted in a substantial increase in milk trans-10 18:1 concentration (5.4% total FA), with considerable variation between individual animals. Relative to the control and EL, milk fat responses to ELFO were characterized by increases (P<0.05) in milk trans-16:1 (Δ9 to 11), trans-18:1 (Δ6 to 11), trans-18:2, CLA (cis-9,trans-11, trans-9,cis-11, trans-8,trans-10 and trans-7,trans-9) and 20- and 22-carbon FA concentrations. Overall, EL resulted in a relatively high cis-9 18:1 concentration and an increase in the 18:3n-3/18:2n-6 ratio, whereas combining EL and FO resulted in substantial increases in trans-FA, marginal enrichment in 20:5n-3 and 22:6n-3 and lower 16:0 concentration changes associated with a decrease in milk fat content. In conclusion, data provide further evidence of differential mammary lipogenic responses to diet in the goat compared with the cow and sheep.

Keywords

goat milkextruded linseedfish oilCLAtrans fatty acid
Type
Research Article
Information
animal , Volume 9 , Issue 5 , May 2015 , pp. 810 - 821
Copyright
© The Animal Consortium 2014 

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References

Association of Official Analytical Chemists (AOAC) 1997. Official methods of analysis, 16th edition. AOAC, Arlington, VA, USA.Google Scholar
Bernard, L, Shingfield, KJ, Rouel, J, Ferlay, A and Chilliard, Y 2009. Effect of plant oils in the diet on performance and milk fatty acid composition in goats fed diets based on grass hay or maize silage. British Journal of Nutrition 101, 213–224.Google Scholar
Bernard, L, Rouel, J, Leroux, C, Ferlay, A, Faulconnier, Y, Legrand, P and Chilliard, Y 2005. Mammary lipid metabolism and milk fatty acid secretion in alpine goats fed vegetable lipids. Journal of Dairy Science 88, 1478–1489.Google Scholar
Burns, TA, Kadegowda, AKG, Duckett, SK, Pratt, SL and Jenkins, TC 2012. Palmitoleic (16:1 cis-9) and cis-vaccenic (18:1 cis-11) acid alter lipogenesis in bovine adipocyte cultures. Lipids 47, 1143–1153.Google Scholar
Chilliard, Y and Ferlay, A 2004. Dietary lipids and forages interactions on cow and goat milk fatty acid composition and sensory properties. Reproduction Nutrition Development 44, 467–492.Google Scholar
Chilliard, Y, Ferlay, A and Doreau, M 2001. Effect of different types of forages, animal fat or marine oils in cow’s diet on milk fat secretion and composition, especially conjugated linoleic acid (CLA) and polyunsaturated fatty acids. Livestock Production Science 70, 31–48.Google Scholar
Chilliard, Y, Rouel, J and Guillouet, P 2013. Goat alpha-s1 casein genotype interacts with the effect of extruded linseed feeding on milk fat yield, fatty acid composition and post-milking lipolysis. Animal Feed Science and Technology 185, 140–149.Google Scholar
Chilliard, Y, Ferlay, A, Rouel, J and Lamberet, G 2003. A review of nutritional and physiological factors affecting goat milk lipid synthesis and lipolysis. Journal of Dairy Science 86, 1751–1770.Google Scholar
Chilliard, Y, Glasser, F, Ferlay, A, Bernard, L, Rouel, J and Doreau, M 2007. Diet, rumen biohydrogenation and nutritional quality of cow and goat milk fat. European Journal of Lipid Science and Technology 109, 828–855.Google Scholar
Eknæs, M, Havrevoll, Ø, Volden, H and Hove, K 2009. Fat content, fatty acid profile and off-flavours in goats milk – effects of feed concentrates with different fat sources during the grazing season. Animal Feed Science and Technology 152, 112–122.Google Scholar
Ferlay, A, Doreau, M, Martin, C and Chilliard, Y 2013. Effects of incremental amounts of extruded linseed on the milk fatty acid composition of dairy cows receiving hay or corn silage. Journal of Dairy Science 96, 6577–6595.Google Scholar
Gagliostro, GA, Rodríguez, A, Pellegrini, PA, Gatti, P, Muset, G, Castañeda, RA, Colombo, D and Chilliard, Y 2006. Effects of fish oil or sunflower plus fish oil supplementation on conjugated linoleic acid (CLA) and omega 3 fatty acids in goat milk. Revista Argentina de Produccion Animal 26, 71–87.Google Scholar
Gómez-Cortés, P, Bach, A, Luna, P, Juárez, M and de la Fuente, MA 2009. Effects of extruded linseed supplementation on n-3 fatty acids and conjugated linoleic acid in milk and cheese from ewes. Journal of Dairy Science 92, 4122–4134.Google Scholar
INRA 1989. Ruminant nutrition. In Recommended allowances and feed tables(ed. R Jarrige), pp. 15–21. John Libbey Eurotext, London, England, Paris, France.Google Scholar
Jacome-Sosa, MM, Jing, L, Ye, W, Ruth, MR, Wright, DC, Reaney, MJ, Jianheng, S, Field, CJ, Vine, DF and Proctor, SD 2010. Increased hypolipidemic benefits of cis-9, trans-11 conjugated linoleic acid in combination with trans-11 vaccenic acid in a rodent model of the metabolic syndrome, the JCR:LA-cp rat. Nutrition & Metabolism 7, 1–10.Google Scholar
Kairenius, P, Toivonen, V and Shingfield, KJ 2011. Identification and ruminal outflow of long-chain fatty acid biohydrogenation intermediates in cows fed diets containing fish oil. Lipids 46, 587–606.Google Scholar
Kitessa, SM, Gulati, SK, Ashes, JR, Fleck, E, Scott, TW and Nichols, PD 2001. Utilisation of fish oil in ruminants – II. Transfer of fish oil fatty acids into goats’ milk. Animal Feed Science and Technology 89, 201–208.Google Scholar
Koops, J and Klomp, H 1977. Rapid colorimetric determination of free fatty acids (lipolysis) in milk by the copper soap method. Netherlands Milk and Dairy Journal 31, 56–72.Google Scholar
Lee, MRF, Shingfield, KJ, Tweed, JKS, Toivonen, V, Huws, SA and Scollan, ND 2008. Effect of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids in steers fed grass or red clover silages. Animal 2, 1859–1869.Google Scholar
Lerch, S, Shingfield, KJ, Ferlay, A, Vanhatalo, A and Chilliard, Y 2012a. Rapeseed or linseed in grass-based diets: effects on conjugated linoleic and conjugated linolenic acid isomers in milk fat from Holstein cows over 2 consecutive lactations. Journal of Dairy Science 95, 7269–7287.Google Scholar
Lerch, S, Ferlay, A, Shingfield, KJ, Martin, B, Pomiès, D and Chilliard, Y 2012b. Rapeseed or linseed supplements in grass-based diets: effects on milk fatty acid composition of Holstein cows over two consecutive lactations. Journal of Dairy Science 95, 5221–5241.Google Scholar
Loor, JJ, Doreau, M, Chardigny, JM, Ollier, A, Sebedio, JL and Chilliard, Y 2005. Effects of ruminal or duodenal supply of fish oil on milk fat secretion and profiles of trans-fatty acids and conjugated linoleic acid isomers in dairy cows fed maize silage. Animal Feed Science and Technology 119, 227–246.CrossRefGoogle Scholar
Martínez Marín, AL, Gómez-Cortés, P, Gómez Castro, AG, Juárez, M, Pérez Alba, LM, Pérez Hernández, M and de la Fuente, MA 2011. Animal performance and milk fatty acid profile of dairy goats fed diets with different unsaturated plant oils. Journal of Dairy Science 94, 5359–5368.CrossRefGoogle ScholarPubMed
Mele, M, Buccioni, A, Serra, A, Antongiovanni, M and Secchiari, P 2008. Lipids of goat’s milk: origin, composition and sources of variation. In Dairy goats feeding and nutrition (ed. A Cannas and G Pulina), pp. 47–70. CAB International, Wallingford, United Kingdom.CrossRefGoogle Scholar
Nudda, A, Battacone, G, Usai, MG, Fancellu, S and Pulina, G 2006. Supplementation with extruded linseed cake affects concentrations of conjugated linoleic acid and vaccenic acid in goat milk. Journal of Dairy Science 89, 277–282.Google Scholar
Offer, NW, Marsden, M, Dixon, J, Speake, BK and Thacker, FE 1999. Effect of dietary fat supplements on levels of n-3 poly-unsaturated fatty acids, trans acids and conjugated linoleic acid in bovine milk. Animal Science 69, 613–625.Google Scholar
Pintus, S, Murru, E, Carta, G, Cordeddu, L, Batetta, B, Accossu, S, Pistis, D, Uda, S, Ghiani, ME, Mele, M, Secchiari, P, Almerighi, G, Pintus, P and Banni, S 2013. Sheep cheese naturally enriched in alpha-linolenic, conjugated linoleic and vaccenic acids improves the lipid profile and reduces anandamide in the plasma of hypercholesterolaemic subjects. British Journal of Nutrition 109, 1453–1462.Google Scholar
Shingfield, KJ, Bernard, L, Leroux, C and Chilliard, Y 2010. Role of trans fatty acids in the nutritional regulation of mammary lipogenesis in ruminants. Animal 4, 1140–1166.Google Scholar
SAS 2000. Statistical Analysis System software package version 9.1. SAS Institute Inc., Cary, NC, USAGoogle Scholar
Shingfield, KJ, Ahvenjarvi, S, Toivonen, V, Vanhatalo, A and Huhtanen, P 2007. Transfer of absorbed cis-9, trans-11 conjugated linoleic acid into milk is biologically more efficient than endogenous synthesis from absorbed vaccenic acid in lactating cows. Journal of Nutrition 137, 1154–1160.Google Scholar
Shingfield, KJ, Chilliard, Y, Toivonen, V, Kairenius, P and Givens, DI 2008. Trans fatty acids and bioactive lipids in ruminant milk. Advances in Experimental Medicine and Biology 606, 3–65.CrossRefGoogle ScholarPubMed
Shingfield, KJ, Reynolds, CK, Hervás, G, Griinari, JM, Grandison, AS and Beever, DE 2006. Examination of the persistency of milk fatty acid composition responses to fish oil and sunflower oil in the diet of dairy cows. Journal of Dairy Science 89, 714–732.Google Scholar
Shingfield, KJ, Ahvenjärvi, S, Toivonen, V, Äröla, A, Nurmela, KVV, Huhtanen, P and Griinari, JM 2003. Effect of dietary fish oil on biohydrogenation of fatty acids and milk fatty acid content in cows. Animal Science 77, 165–179.Google Scholar
Shingfield, KJ, Reynolds, CK, Lupoli, B, Toivonen, V, Yurawecz, MP, Delmonte, P, Griinari, JM, Grandison, AS and Beever, DE 2005. Effect of forage type and proportion of concentrate in the diet on milk fatty acid composition in cows given sunflower oil and fish oil. Animal Science 80, 225–238.Google Scholar
Shingfield, KJ, Kairenius, P, Ärölä, A, Paillard, D, Muetzel, S, Ahvenjärvi, S, Vanhatalo, A, Huhtanen, P, Toivonen, V, Griinari, JM and Wallace, RJ 2012. Dietary fish oil supplements modify ruminal biohydrogenation, alter the flow of fatty acids at the omasum, and induce changes in the ruminal butyrivibrio population in lactating cows. Journal of Nutrition 142, 1437–1448.Google Scholar
Toral, PG, Rouel, J, Bernard, L and Chilliard, Y 2014. Interaction between fish oil and plant oils or starchy concentrates in the diet: effects on dairy performance and milk fatty acid composition in goats. Animal Feed Science and Technology 198, 67–82.Google Scholar
Toral, PG, Frutos, P, Hervás, G, Gómez-Cortés, P, Juárez, M and de la Fuente, MA 2010. Changes in milk fatty acid profile and animal performance in response to fish oil supplementation, alone or in combination with sunflower oil, in dairy ewes. Journal of Dairy Science 93, 1604–1615.CrossRefGoogle ScholarPubMed
Whitlock, LA, Schingoethe, DJ, Hippen, AR, Kalscheur, KF, Baer, RJ, Ramaswamy, N and Kasperson, KM 2002. Fish oil and extruded soybeans fed in combination increase conjugated linoleic acids in milk of dairy cows more than when fed separately. Journal of Dairy Science 85, 234–243.Google Scholar