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Birth of the first mithun (Bos frontalis) calf through artificial insemination

Published online by Cambridge University Press:  13 May 2008

A. Dhali ,
M. Karunakaran ,
A. Mech ,
N. Nath ,
B. Prakash ,
C. Rajkhowa  and
D. P. Mishra
A. Dhali*
Affiliation:
National Research Centre on Mithun, Medziphema, Nagaland 797 106, India
M. Karunakaran
Affiliation:
ICAR-RC-NEH Region, Medziphema, Nagaland 797 106, India
A. Mech
Affiliation:
National Research Centre on Mithun, Medziphema, Nagaland 797 106, India
N. Nath
Affiliation:
National Research Centre on Mithun, Medziphema, Nagaland 797 106, India
B. Prakash
Affiliation:
National Research Centre on Mithun, Medziphema, Nagaland 797 106, India
C. Rajkhowa
Affiliation:
National Research Centre on Mithun, Medziphema, Nagaland 797 106, India
D. P. Mishra
Affiliation:
National Research Centre on Mithun, Medziphema, Nagaland 797 106, India
*
E-mail: [email protected]
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Abstract

The study describes the standardization of a suitable semen cryopreservation protocol for the first time in mithun (Bos frontalis) and birth of the first mithun calf through artificial insemination. The semen samples were collected from adult bulls through the rectal massage method and cryopreserved in liquid nitrogen using tris–egg yolk–glycerol diluent. The diluted semen samples were packaged in 0.50 ml straws and kept at 5°C for 4 h for equilibration. Following the equilibration, the straws were frozen into liquid nitrogen vapour for 10 min and then plunged into liquid nitrogen for storage. It was observed that the progressive motility (%) decreased significantly (P < 0.01) in cryopreserved semen (43.3 ± 4.1) compared with fresh samples (76.6 ± 3.3). The percentages of live spermatozoa (P < 0.01) and spermatozoa with intact acrosome (P < 0.05) also decreased significantly in cryopreserved semen (54.0 ± 3.3 and 64.6 ± 5.3) compared with fresh samples (79.3 ± 2.6 and 85.3 ± 1.8). Simultaneously, the total morphological abnormality (%) was found to be significantly (P < 0.01) higher in cryopreserved samples (15.46 ± 2.68) than in fresh semen (3.85 ± 0.63). A total of three mithun cows were inseminated using the cryopreserved semen. All the cows conceived following insemination and gave birth to healthy calves. The study revealed that mithun semen can be cryopreserved efficiently using tris–egg yolk–glycerol diluent, which can be further used for artificial insemination.

Keywords

semencryopreservationtris–egg yolk–glycerolmithunAI
Type
Full Paper
Information
animal , Volume 2 , Issue 6 , June 2008 , pp. 879 - 882
Copyright
Copyright © The Animal Consortium 2008

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