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Performance and egg quality of laying hens fed flaxseed: highlights on n-3 fatty acids, cholesterol, lignans and isoflavones

Published online by Cambridge University Press:  07 November 2016

S. Mattioli ,
S. Ruggeri ,
B. Sebastiani ,
G. Brecchia ,
A. Dal Bosco ,
A. Cartoni Mancinelli  and
C. Castellini
S. Mattioli*
Affiliation:
Department of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX Giugno 74, 06100 Perugia, Italy
S. Ruggeri
Affiliation:
Department of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX Giugno 74, 06100 Perugia, Italy
B. Sebastiani
Affiliation:
Department of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di sotto 8, 06100 Perugia, Italy
G. Brecchia
Affiliation:
Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06100 Perugia, Italy
A. Dal Bosco
Affiliation:
Department of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX Giugno 74, 06100 Perugia, Italy
A. Cartoni Mancinelli
Affiliation:
Department of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX Giugno 74, 06100 Perugia, Italy
C. Castellini
Affiliation:
Department of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX Giugno 74, 06100 Perugia, Italy
*
†E-mail: [email protected]
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Abstract

Flaxseed is a rich source of α-linolenic acid and phytoestrogens, mainly lignans, whose metabolites (enterodiol and enterolactone) can affect estrogen functions. The present study evaluated the influence of dietary flaxseed supplementation on reproductive performance and egg characteristics (fatty acids, cholesterol, lignans and isoflavones) of 40 Hy-Line hens (20/group) fed for 23 weeks a control diet or the same diet supplemented with 10% of extruded flaxseed. The flaxseed diet had approximately three times the content of lignans (2608.54 ng/g) as the control diet, mainly secoisolariciresinol diglucoside (1534.24 v. 494.72 ng/g). When compared with the control group, hens fed flaxseed showed a similar deposition rate (72.0% v. 73.9%) and egg yield. Furthermore, there was no effect of flaxseed on the main chemical composition of the egg and on its cholesterol content. Estradiol was higher in the plasma of the control group (1419.00 v. 1077.01 pg/ml) probably due to the effect of flaxseed on phytoestrogen metabolites. The plasma lignans were higher in hens fed flaxseed, whereas isoflavones were lower, mainly due to the lower equol value (50.52 v. 71.01 ng/ml). A similar trend was shown in eggs: the flaxseed group had higher level of enterodiol and enterolactone, whereas the equol was lower (198.31 v. 142.02 ng/g yolk). Secoisolariciresinol was the main lignan in eggs of the flaxseed group and its concentration was three times higher then control eggs. Flaxseed also improved the n-3 long-chain polyunsaturated fatty acids of eggs (3.25 v. 0.92 mg/g egg), mainly DHA, however, its oxidative status (thiobarbituric reactive substances) was negatively affected. In conclusion, 10% dietary flaxseed did not affect the productive performance of hens or the yolk cholesterol concentration, whereas the lignans and n-3 polyunsaturated fatty acid content of eggs improved. Further details on the competition between the different dietary phytoestrogens and their metabolites (estrogen, equol, enterodiol and enterolactone) should be investigated.

Keywords

flaxseedisoflavoneslignansequolegg quality
Type
Research Article
Information
animal , Volume 11 , Issue 4 , April 2017 , pp. 705 - 712
Copyright
© The Animal Consortium 2016 

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References

Association of Official Analytical Chemists 1995. Official methods of analysis, 15th edition. AOAC, Arlington, VA, USA. pp. 931–948.Google Scholar
Bean, LD and Leeson, S 2003. Long-term effects of feeding flaxseed on performance and egg fatty acid composition of brown and white hens. Poultry Science 82, 388–394.CrossRefGoogle Scholar
Brooks, JD and Thompson, LU 2005. Mammalian lignans and genistein decrease the activities of aromatase and 17β-hydroxysteroid dehydrogenase in MCF-7 cells. The Journal of Steroid Biochemistry and Molecular Biology 94, 461–467.CrossRefGoogle Scholar
Carreau, C, Flouriot, G, Bennetau-Pelissero, C and Potier, M 2008. Enterodiol and enterolactone, two major diet-derived polyphenol metabolites have different impact on ERa transcriptional activation in human breast cancer cells. Journal of Steroid Biochemistry and Molecular Biology 110, 176–185.CrossRefGoogle Scholar
Cherian, G and Hayat, Z 2009. Long-term effects of feeding flaxseeds on hepatic lipid characteristics and histopathology of laying hens. Poultry Science 88, 2555–2561.Google Scholar
Dikshit, A, Drião Gomes Filho, MA, Eilati, E, McGee, S, Small, C, Gao, C, Klug, T and Buchanan Hales, D 2015. Flaxseed reduces the pro-carcinogenic micro-environment in the ovaries of normal hens by altering the PG and estrogen pathways in a dose-dependent manner. British Journal of Nutrition 113, 1384–1395.Google Scholar
Evans, BA, Griffiths, K and Morton, MS 1995. Inhibition of 5 a-reductase in genital skin fibroblasts and prostate tissue by dietary lignans and isoflavonoids. Journal of Endocrinology 147, 295–302.CrossRefGoogle Scholar
Folch, J, Lees, M and Sloane-Stanley, GH 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Galobart, J, Barroeta, AC, Baucells, MD and Guardiola, F 2001. Lipid oxidation in fresh and spray-dried eggs enriched with ω3 and ω6 polyunsaturated fatty acids during storage as affected by dietary vitamin E and canthaxanthin supplementation. Poultry Science 80, 327–337.CrossRefGoogle Scholar
Jenkins, DJ, Kendall, CW, Vidgen, E, Agarwal, S, Rao, AV, Rosenberg, RS and Griffin, LC 1999. Health aspects of partially defatted flaxseed, including effects on serum lipids, oxidative measures, and ex vivo androgen and progestin activity: a controlled crossover trial. The American Journal of Clinical Nutrition 69, 395–402.Google Scholar
Kennedy, AK 1997. Effects of flaxseed lignans on laying hen reproductive parameters. Doctoral dissertation, Texas A&M University, College Station, TX, USA.Google Scholar
Kładna, A, Berczyński, P, Kruk, I, Piechowska, T and Aboul‐Enein, HY 2016. Studies on the antioxidant properties of some phytoestrogens. Luminescence 31, 1201–1206.CrossRefGoogle Scholar
Mattioli, S, Dal Bosco, A, Martino, M, Ruggeri, S, Marconi, O, Sileoni, V, Falcinelli, B, Castellini, C and Benincasa, P 2016. Alfalfa and flax sprouts supplementation enriches the content of bioactive compounds and lowers the cholesterol in hen egg. Journal of Functional Foods 22, 454–462.Google Scholar
Meagher, LP and Beecher, GR 2000. Assessment of data on the lignin content of foods. Journal of Food Composition and Analysis 13, 935–947.Google Scholar
Milinsk, MC, Murakami, AE, Gomes, STM, Matsushita, M and Desouza, NE 2003. Fatty acid profile of egg yolk lipids from hens fed diets rich in n-3 fatty acids. Food Chemistry 83, 287–292.Google Scholar
Morton, M, Wilcox, G, Wahlqvist, M and Griffiths, K 1994. Determination of lignans and isoflavonoids in human female plasma following dietary supplementation. Journal of Endocrinology 142, 251–259.Google Scholar
Mousavi, Y and Adlercreutz, H 1992. Enterolactone and estradiol inhibit each other’s proliferative effect on MCF-7 breast cancer cells in culture. Journal of Steroid Biochemistry and Molecular Biology 41, 615–619.Google Scholar
Murray, T, Kang, J, Astheimer, L and Price, WE 2007. Tissue distribution of lignans in rats in response to diet, dose-response, and competition with isoflavones. Journal of Agricultural and Food Chemistry 55, 4907–4912.Google Scholar
National Research Council 1994. Nutrient requirements of poultry, 9th edition. National Academy of Sciences, Berkeley, CA, USA.Google Scholar
Novak, C and Scheideler, SE 2001. Long-term effects of feeding flaxseed-based diets. 1. Egg production parameters, components, and eggshell quality in two strains of laying hens. Poultry Science 80, 1480–1489.Google Scholar
Oomah, BD 2001. Flaxseed as a functional food source. Journal of the Science of Food and Agriculture 81, 889–894.CrossRefGoogle Scholar
Palmiter, RD, Oka, T and Schimke, RT 1971. Modulation of ovalbumin synthesis by estradiol-17β and actinomycin D as studied in explants of chick oviduct in culture. Journal of Biological Chemistry 246, 724–737.CrossRefGoogle ScholarPubMed
Petit, HV and Twagiramungu, H 2006. Conception rate and reproductive function of dairy cows fed different fat sources. Theriogenology 66, 1316–1324.Google Scholar
Phipps, WR, Martini, MC, Lampe, JW, Slavin, JL and Kurzer, MS 1993. Effect of flax seed ingestion on the menstrual cycle. Journal of Clinical Endocrinology and Metabolism 77, 1215–1219.Google ScholarPubMed
Prasad, K and Jadhav, A 2016. Prevention and treatment of atherosclerosis with flaxseed-derived compound secoisolariciresinol diglucoside. Current Pharmaceutical Design 22, 214–220.Google Scholar
Scheideler, SE and Froning, GW 1996. The combined influence of dietary flaxseed variety, level, form, and storage conditions on egg production and composition among vitamin E-supplemented hens. Poultry Science 75, 1221–1226.Google Scholar
Shultz, T, Bonorden, W and Seaman, W 1991. Effect of short-term flaxseed consumption on lignan and sex hormone metabolism. Nutrition Research 11, 1089–1100.Google Scholar
Setchell, KDR, Borriello, SP, Gordon, H, Lawson, AM, Harkness, R, Morgan, DML and Axelson, M 1981. Lignan formation in man – microbial involvement and possible roles in relation to cancer. The Lancet 318, 4–7.Google Scholar
Setchell, KDR, Brown, NM and Lydeking-Olsen, E 2002. The clinical importance of the metabolite equol – a clue to the effectiveness of soy and its isoflavones. Journal of Nutrition 132, 3577–3584.Google Scholar
Setchell, KDR and Cole, SJ 2003. Variations in isoflavone levels in soy foods and soy protein isolates and issues related to isoflavone databases and food labeling. Journal of Agriculture and Food Chemistry 51, 4146–4155.Google Scholar
Speckman, SC, Hales, DB and Hales, KH 2012. Investigating effects of flaxseed on expression of mir-200a, ZEB1, and E-Cadherin in ovarian cancer in the laying hen. Biology of Reproduction 87, 546.CrossRefGoogle Scholar
STATA 2015. Statistical software, version 14. StataCorp, College Station, TX, USA.Google Scholar
Vaghefi, SB 2002. Eggs and health: myths and misconceptions. In Eggs and Health Promotion (ed. RR Watson), pp. 83–100. Iowa State Press, Ames, IA, USA.Google Scholar
Van Elswyk, ME 1997. Nutritional and physiological effects of flax seed in diets for laying fowl. World’s Poultry Science Journal 53, 253–264.Google Scholar
Vuilleumier, JP 1969. The ‘Roche yolk colour fan’ – an instrument for measuring yolk colour. Poultry Science 48, 767–779.Google Scholar