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The effects of seaweed extract inclusion on gut morphology, selected intestinal microbiota, nutrient digestibility, volatile fatty acid concentrations and the immune status of the weaned pig

Published online by Cambridge University Press:  01 October 2008

P. Reilly ,
J. V. O’Doherty ,
K. M. Pierce ,
J. J. Callan ,
J. T. O’Sullivan  and
T. Sweeney
P. Reilly
Affiliation:
School of Agriculture, Food Science, and Veterinary Medicine, College of Life Sciences, University College Dublin, Lyons Research Farm, Newcastle, Co. Dublin, Ireland
J. V. O’Doherty*
Affiliation:
School of Agriculture, Food Science, and Veterinary Medicine, College of Life Sciences, University College Dublin, Lyons Research Farm, Newcastle, Co. Dublin, Ireland
K. M. Pierce
Affiliation:
School of Agriculture, Food Science, and Veterinary Medicine, College of Life Sciences, University College Dublin, Lyons Research Farm, Newcastle, Co. Dublin, Ireland
J. J. Callan
Affiliation:
School of Agriculture, Food Science, and Veterinary Medicine, College of Life Sciences, University College Dublin, Lyons Research Farm, Newcastle, Co. Dublin, Ireland
J. T. O’Sullivan
Affiliation:
Bioatlantis Ltd, Kerry Technology Park, Tralee, Kerry, Ireland
T. Sweeney
Affiliation:
School of Agriculture, Food Science, and Veterinary Medicine, College of Life Sciences, University College Dublin, Lyons Research Farm, Newcastle, Co. Dublin, Ireland
*
E-mail: [email protected]
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Abstract

An experiment (complete randomised design) was conducted to investigate the effects of Laminaria hyperborea and Laminaria digitata seaweed extract inclusion on gut morphology, selected intestinal microbiota populations, volatile fatty acid concentrations and the immune status of the weaned pig. Twenty-eight piglets (24 days of age, 6.5 ± 1.4 kg live weight) were assigned to one of four dietary treatments for 7 days and then sacrificed: (T1) basal diet (control); (T2) basal diet and 1.5 g/kg L. hyperborea seaweed extract; (T3) basal diet and 1.5 g/kg L. digitata seaweed extract; and (T4) basal diet and 1.5 g/kg of a combination of L. hyperborea and L. digitata seaweed extract. The seaweed extract contained both laminarin and fucoidan. Digesta samples were taken from the caecum and colon to measure the enterobacteria, bifidobacteria and lactobacilli populations and for volatile fatty acid analysis. Tissue samples were taken from the duodenum, jejunum and ileum for morphological examination. Blood samples were taken to determine the cytokine gene expression profile and to measure the phagocytotic capacity of the blood. Pigs offered diets containing L. hyperborea seaweed extract had less bifidobacteria in the colon (P < 0.05) and lactobacilli in the caecum (P < 0.05) and colon (P < 0.001). The inclusion of L. digitata seaweed extract resulted in lower populations of enterobacteria in the caecum and colon (P < 0.01), bifidobacteria in the caecum (P < 0.05), and lactobacilli in the caecum (P < 0.05) and colon (P < 0.001). Pigs offered the combination of L. hyperborea and L. digitata seaweed extracts had less enterobacteria (P < 0.05) and lactobacilli (P < 0.01) in the caecum and colon. Pigs offered the L. digitata-supplemented diet had a reduced villous height in the duodenum and jejunum (P < 0.05). The inclusion of the L. digitata seaweed extract increased the molar proportion of butyric acid in the colon (P < 0.05). There was a significant reduction in the ammonia concentration in the colon with the inclusion of L. hyperborea (P < 0.01) and L. digitata (P < 0.05) seaweed extracts. An increase in the expression of the Interleukin-8 mRNA was observed on day 6 with the supplementation of the combination of L. hyperborea and L. digitata seaweed extract (P < 0.05). The inclusion of L. hyperborea seaweed extract resulted in an increase in total monocyte number (P < 0.05). In conclusion, the supplementation of L. hyperborea and L. digitata seaweed extract alone and in combination reduced the enterobacteria, bifidobacteria and lactobacilli populations in the caecum and colon, while only marginal effects on the immune response was observed.

Keywords

fucoidanimmunitylaminarinmicrobiotapig
Type
Full Paper
Information
animal , Volume 2 , Issue 10 , October 2008 , pp. 1465 - 1473
Copyright
Copyright © The Animal Consortium 2008

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