Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-18T16:48:01.189Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect and mode of action of the Texel muscling QTL (TM-QTL) on carcass traits in purebred Texel lambs

Published online by Cambridge University Press:  07 May 2014

J. M. Macfarlane ,
N. R. Lambe ,
O. Matika ,
P. L. Johnson ,
B. T. Wolf ,
W. Haresign ,
S. C. Bishop  and
L. Bünger
J. M. Macfarlane
Affiliation:
Animal and Veterinary Sciences Group, Scotland’s Rural College (SRUC), West Mains Road, Edinburgh, EH9 3JG, UK
N. R. Lambe
Affiliation:
Animal and Veterinary Sciences Group, Scotland’s Rural College (SRUC), West Mains Road, Edinburgh, EH9 3JG, UK
O. Matika
Affiliation:
The Roslin Institute and R(D)SVS, University of Edinburgh, Midlothian, EH25 9RG, UK
P. L. Johnson
Affiliation:
AgResearch Invermay, Puddle Alley, Private Bag 50034, Mosgiel, New Zealand
B. T. Wolf
Affiliation:
Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Penglais Campus, Aberystwyth, Ceredigion, SY23 3FG, UK
W. Haresign
Affiliation:
Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Penglais Campus, Aberystwyth, Ceredigion, SY23 3FG, UK
S. C. Bishop
Affiliation:
The Roslin Institute and R(D)SVS, University of Edinburgh, Midlothian, EH25 9RG, UK
L. Bünger*
Affiliation:
Animal and Veterinary Sciences Group, Scotland’s Rural College (SRUC), West Mains Road, Edinburgh, EH9 3JG, UK
*
†E-mail: [email protected]
Get access

Abstract

TM-QTL is a quantitative trait locus (QTL) on ovine chromosome 18 (OAR18) known to affect loin muscling in Texel sheep. Previous work suggested that its mode of inheritance is consistent with paternal polar overdominance, but this has yet to be formally demonstrated. This study used purebred Texel sheep segregating for TM-QTL to confirm its presence in the chromosomal region in which it was first reported and to determine its pattern of inheritance. To do so, this study used the first available data from a Texel flock, which included homozygote TM-QTL carriers (TM/TM; n=34) in addition to homozygote non-carriers (+/+; n=40 and, heterozygote TM-QTL-carriers inheriting TM-QTL from their sire (TM/+; n=53) or their dam (+/TM; n=17). Phenotypes included a wide range of loin muscling, carcass composition and tissue distribution traits. The presence of a QTL affecting ultrasound muscle depth on OAR18 was confirmed with a paternal QTL effect ranging from +0.54 to +2.82 mm UMD (s.e. 0.37 to 0.57 mm) across the sires segregating for TM-QTL. Loin muscle width, depth and area, loin muscle volume and dissected M. longissimus lumborum weight were significantly greater for TM/+ than +/+ lambs (+2.9% to +7.9%; P<0.05). There was significant evidence that the effect of TM-QTL on the various loin muscling traits measured was paternally polar overdominant (P<0.05). In contrast, there was an additive effect of TM-QTL on both live weight at 20 weeks and carcass weight; TM/TM animals were significantly (P<0.05) heavier than +/+ (+11.1% and +7.3%, respectively) and +/TM animals (+11.9% and +11.7%, respectively), with TM/+ intermediate. Weights of the leg, saddle and shoulder region (corrected for carcass weight) were similar in the genotypic groups. There was a tendency for lambs inheriting TM-QTL from their sire to be less fat with slightly more muscle than non-carriers. For example, carcass muscle weight measured by live animal CT-scanning was 2.8% higher in TM/TM than +/+ lambs (P<0.05), carcass muscle weight measured by carcass CT-scanning was 1.36% higher in TM/+ than +/+ lambs (P<0.05), and weight of fat trimmed from the carcass cuts was significantly lower for TM/+ than +/+ lambs (−11.2%; P<0.05). No negative effects of TM-QTL on carcass traits were found. Optimal commercial use of TM-QTL within the sheep industry would require some consideration, due to the apparently different mode of action of the two main effects of TM-QTL (on growth and muscling).

Keywords

geneticsQTLsheepTexelmuscling
Type
Full Paper
Information
animal , Volume 8 , Issue 7 , July 2014 , pp. 1053 - 1061
Copyright
© The Animal Consortium 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bunger, L, Macfarlane, JM, Lambe, NR, Conington, J, McLean, KA, Moore, K, Glasbey, CA and Simm, G 2011. Use of X-ray computed tomography (CT) in UK sheep production and breeding. In CT scanning – techniques and applications (ed. S Karuppasamy), pp. 329–348. INTECH Open Access Publisher, Rijeka, Croatia.Google Scholar
Charlier, C, Segers, K, Wagenaar, D, Karim, L, Berghams, S, Jaillon, O, Shay, T, Weissenbach, J, Cockett, N, Gyapay, G and Georges, M 2001a. Human-ovine comparative sequencing of a 250-kb imprinted domain encompassing the callipyge (clpg) locus and identification of six imprinted transcripts: DLK1, DAT, GTL2, PEG11, antiPEG11, and MEG8. Genome Research 11, 850–862.CrossRefGoogle ScholarPubMed
Charlier, C, Segers, K, Wagenaar, D, Karim, L, Berghmans, S, Jaillon, O, Shay, T, Weissenbach, J, Cockett, N, Gyapay, G and Georges, M 2001b. Human-ovine comparative sequencing of a 250-kb imprinted domain encompassing the callipyge (clpg) locus and identification of six imprinted transcripts: DLK1, DAT, GTL2, PEG11, antiPEG11, and MEG8. Genome Research 11, 850–862.Google Scholar
Churchill, GA and Doerge, RW 1994. Empirical threshold values for quantitative trait mapping. Genetics 138, 963–971.CrossRefGoogle ScholarPubMed
Cockett, NE, Jackson, SP, Snower, GD, Shay, TL, Berghmans, S, Beever, JE, Carpenter, C and Georges, M 1996a. Polar overdominance at the ovine callipyge locus. Journal of Animal Science 77 (suppl. 2), 221–227.CrossRefGoogle Scholar
Cockett, NE, Jackson, SP, Shay, TL, Farnir, F, Berghmans, S, Snowder, GD, Nielsen, DM and Georges, M 1996b. Polar overdominance at the Ovine callipyge locus. Science 273, 236–238.Google Scholar
Cockett, NE, Jackson, SP, Shay, TL, Nielsen, D, Moore, SS, Steel, MR, Barendse, W, Green, RD and Georges, M 1994a. Chromosomal localization of the callipyge gene in sheep (Ovis aries) using bovine DNA markers. Proceedings of the National Academy of Sciences of the United States of America 91, 3019–3023.CrossRefGoogle ScholarPubMed
Cockett, NE, Jackson, SP, Shay, TL, Nielsen, D and Moore, SS 1994b. Chromosomal localization of the callipyge gene in sheep (Ovis aries) using bovine DNA markers. [ABA 62, 5543]. Proceedings of the National Academy of Sciences of the United States of America 91, 3019–3023.Google Scholar
Freking, BA, Keele, JW, Shackelford, SD, Wheeler, TL, Koohmaraie, M, Nielsen, MK and Leymaster, KA 1999. Evaluation of the ovine callipyge locus: III. Genotypic effects on meat quality traits. Journal of Animal Science 77, 2336–2344.Google Scholar
Freking, BA, Keele, JW, Beattie, CW, Kappes, SM, Smith, TP, Sonstegard, TS, Nielsen, MK and Leymaster, KA 1998. Evaluation of the ovine callipyge locus: I. Relative chromosomal position and gene action. Journal of Animal Science 76, 2062–2071.CrossRefGoogle ScholarPubMed
Freking, BA, Murphy, SK, Wylie, AA, Rhodes, SJ, Keele, JW, Leymaster, KA, Jirtle, RL and Smith, TP 2002. Identification of the single base change causing the callipyge muscle hypertrophy phenotype, the only known example of polar overdominance in mammals. Genome Research 12, 1496–1506.Google Scholar
Garg, P, Borel, C and Sharp, AJ 2012. Detection of parent-of-origin specific expression quantitative trait loci by cis-association analysis of gene expression in trios. PLoS One 7, e41695.CrossRefGoogle ScholarPubMed
GenStat 11 Committee. 2008. GenStat. Lawes Agricultural Trust, Rothamstead Experimental Station, Harpenden, UK.Google Scholar
Georges, M and Cockett, N 1996. The ovine callipyge locus: a paradigm illustrating the importance of non-Mendelian genetics in livestock. Reproduction Nutrition Development 36, 651–657.Google Scholar
Jones, HE, Lewis, RM, Young, MJ and Wolf, BT 2002. The use of X-ray computer tomography for measuring the muscularity of live sheep. Animal Science 75, 387–399.Google Scholar
Jopson, NB, Nicoll, GB, Stevenson-Barry, JM, Duncan, S, Greer, GJ, Bain, WE, Gerard, EM, Glass, BC, Broad, TE and Mcewan, JC 2001. Mode of inheritance and effects on meat quality of the rib-eye muscling (REM) QTL in Sheep. Proceedings of the Association for the Advancement of Animal Breeding and Genetics 14, 111–114.Google Scholar
Knott, SA, Elsen, JM and Haley, CS 1996. Methods for multiple-marker mapping of quantitative trait loci in half-sib populations. Theoretical and Applied Genetics 93, 71–80.Google Scholar
Lambe, NR, Richardson, RI, Macfarlane, JM, Nevison, I, Haresign, W, Matika, O and Bunger, L 2011. Genotypic effects of the Texel muscling QTL (TM-QTL) on meat quality in purebred Texel lambs. Meat Science 89, 125–132.CrossRefGoogle ScholarPubMed
Macfarlane, JM, Lambe, NR, Haresign, W and Bunger, L 2012. The effect of the Texel muscling QTL (TM-QTL) on live and carcass weight in Texel lambs. Small Ruminant Research 85, 715–720.Google Scholar
Macfarlane, JM, Lambe, NR, Bishop, SC, Matika, O, Rius-Vilarrasa, E, McLean, KA, Haresign, W, Wolf, BT, McLaren, RJ and Bunger, L 2009. Effects of the Texel muscling quantitative trait locus on carcass traits in crossbred lambs. Animal 3, 189–199.Google Scholar
Macfarlane, JM, Lambe, NR, Matika, O, McLean, KA, Masri, AY, Johnson, PL, Wolf, BT, Haresign, W, Bishop, SC and Bunger, L 2010. Texel loin muscling QTL (TM-QTL) located on ovine chromosome 18 appears to exhibit imprinting and polar overdominance. Proceedings of the 9th World Congress on Genetics Applied to Livestock Production, Leipzig abstract, p. 199.Google Scholar
Mann, AD, Young, MJ, Glasbey, CA and McLean, KA 2003. STAR: Sheep Tomogram Analysis Routines (V.3.4). Biomathematics and Statistics Scotland, Edinburgh, UK.Google Scholar
Masri, AY, Macfarlane, JM, Lambe, NR, Haresign, W, Brotherstone, S and Bunger, L 2011. Evaluating the effects of the c.*1232G>a mutation and TM-QTL in Texel×Welsh Mountain lambs using ultrasound and video image analyses. Small Ruminant Research 99, 99–109.CrossRefGoogle Scholar
Matika, O, Sechi, S, Pong-Wong, R, Houston, RD, Clop, A, Wooliams, JA and Bishop, SC 2011. Characterization of OAR1 and OAR18 QTL associated with muscle depth in British commercial terminal sire sheep. Animal Genetics 42, 172–180.CrossRefGoogle ScholarPubMed
McLaren, RJ, Mcewan, JC, For, R, Glass, BC, Broad, TE, Greer, GJ and Nicoll, GB 2003. Recombination breakpoint mapping of the Carwell locus for rib-eye muscling in sheep. Proceedings of the International Congress of Genetics XIX, Melbourne, Australia, 6 to 11 July 2003, Abstract, p. 01064.Google Scholar
Navajas, EA, Glasbey, CA, McLean, KA, Fisher, AV, Charteris, AJL, Lambe, NR, Bunger, L and Simm, G 2006. In vivo measurements of muscle volume by automatic image analysis of spiral computed tomography scans. Animal Science 82, 545–553.Google Scholar
Navajas, EA, Lambe, NR, McLean, KA, Glasbey, CA, Fisher, AV, Charteris, AJL, Bunger, L and Simm, G 2007. Accuracy of in vivo muscularity indices measured by computed tomography and their association with carcass quality in lambs. Meat Science 75, 533–542.Google Scholar
Nicoll, GB 2007. The Landcorp Carwell Experience. Proceedings of the Sheep Breeders’ Roundtable 2007, Nottingham, 9 to 11 November 2007.Google Scholar
Rowe, S, Bishop, S and Koning, DJD 2012. Imprinting in genome analysis: modeling parent-of-origin effects in QTL studies. In Livestock epigenetics (ed. H Khatib), pp. 113–129. John Wiley & Sons Inc., Chichester, West Sussex, UK.Google Scholar
Seaton, G, Haley, CS, Knott, SA, Kearsey, M and Visscher, PM 2002. QTL express: mapping quantitative trait loci in of simple and complex pedigrees. Bioinformatics 18, 339–340.Google Scholar
Walling, GA, Visscher, PM, Wilson, AD, McTeir, BL, Simm, G and Bishop, SC 2004. Mapping of quantitative trait loci for growth and carcass traits in commercial sheep populations. Journal of Animal Science 82, 2234–2245.CrossRefGoogle ScholarPubMed
Supplementary material: File

Macfarlane supplementary material

Macfarlane supplementary material

Download Macfarlane supplementary material(File)
File 41.8 KB