This study examined the effects of environmental enrichment on aggressive behaviour and dominance relationships in growing pigs. Three hundred and twenty pigs were reared from birth to 15 weeks of age in either barren or enriched environments. The barren environments were defined by common intensive housing conditions (ie with slatted floors and in terms of recommended space allowances), while the enriched environments incorporated extra space and substrates for manipulation. Aggressive behaviour was observed in a social confrontation test during the suckling period and dominance relationships were assessed from a food competition test at 12 weeks of age. Animals were weighed at regular intervals throughout the experiment. Environmental enrichment reduced the expression of aggressive behaviour. Pigs from enriched rearing environments fought significantly less with unfamiliar animals than those from barren environments when tested under standard conditions (mean of 1.46 vs 2.75 fights per 30min test for enriched vs barren environments; SEM 0.20, P < 0.001). The nature of dominance relationships also appeared to differ between barren and enriched environments. In barren environments, dominance among pen mates was correlated with aggression (r = 0.33, P < 0.01), whereas in enriched environments it was correlated with body weight (r = 0.24, P < 0.01). Correlations between behaviour in the social confrontation and food competition tests suggested that dominance characteristics were established early in life and remained stable through the growing period.

The behaviour of broilers reared at pasture from 4 to 12 weeks of age on a low density diet supplied either indoors or outdoors, was compared with that of birds reared inside on deep litter. This single study found few differences in behaviour. Activity levels of birds outside were initially greater, but from six weeks of age lying increased to comparable levels in all groups.

Surprisingly little use was made of the extra space and facilities such as perches at pasture. It is proposed that the main reason for this was leg weakness as 80 per cent of the birds had a detectable gait abnormality at seven weeks of age. There was no evidence of reduced motivation to extend the behavioural repertoire, as, for example, ground pecking remained at significantly higher levels in the outdoor groups because it could also be performed from a lying posture.

Commercial beef production in western Canada involves raising cows and calves on large tracts of grassland, plus grain-based ‘finishing’ of animals in outdoor feedlots. This study used open-ended, semi-structured interviews to explore views on animal welfare of 23 commercial beef producers in this system. Although wary of the term ‘animal welfare’, participants understood the concept to encompass three well-known elements: (i) basic animal health and body condition; (ii) affective states (comfort, contentment, freedom from hunger or thirst); and (iii) the ability to live a ‘natural’ life. Participants attached importance to protecting animals from natural hardships (extreme weather, predators), yet many regarded some degree of natural challenge as acceptable or even positive. Quiet rumination was uniformly regarded as indicating contentment. Avoiding ‘stress’ was seen as a central goal, to be achieved especially by skilful handling and good facilities. Invasive procedures (branding, castration, de-horning) were recognised as painful but were accepted because they were seen as: (i) necessary for regulatory or management reasons; (ii) satisfactory trade-offs to prevent worse welfare problems such as aggression; or (iii) sufficiently short-term to be relatively unimportant. Other issues — including poor facilities, rough or excessive handling, poor nutrition, and failure to protect health — were regarded as more serious welfare concerns. While feeling constrained by low profits, participants saw good welfare as crucial to profitability. Participants uniformly expressed an ethic of care, enjoyment of working with animals, and varying degrees of willingness to sacrifice personal comfort for animal well-being. We argue that animal welfare policy and advocacy are likely to be more successful in engaging producers if they acknowledge and address producers’ views on animal welfare.

The purpose of this experiment was to investigate the welfare of pregnant mares kept in straight stalls and given only limited exercise, conditions that are similar to those encountered in the pregnant mare urine industry. Sixteen pregnant mares (eight in each of two years) were randomly assigned to two groups: Ex (exercised in a paddock for 30 min per day) or NoEx (exercised for one 30 min period every 14 days). The horses were housed in straight (or ‘tie’) stalls for six months and had ad libitum access to grass hay. Each horse's behaviour was recorded on videotape once per week for 24 h. The major behaviours were eating hay, standing, and stand-resting (head down and one hind limb flexed). There was no difference between the behaviours or the number of foot lifts per min of the Ex and NoEx groups in their stalls. Nine of 16 mares were not observed in recumbency throughout the whole of the six-month observation period, suggesting that horses with no previous experience in straight stalls may be reluctant to lie down. Thirteen of 16 mares dropped to their knees at least once, probably when they were REM sleeping while standing. There were no significant differences between the Ex and the NoEx mares in baseline plasma Cortisol levels or in Cortisol response to ACTH. Following 30 min of exercise, NoEx mares showed an increase in Cortisol from 5.0 to 5.4 μg dL−1, whereas Ex mares showed a decrease from 4.6 to 3.6 µg dL−1. The NoEx horses that had been confined for two weeks trotted more (NoEx = 22 [6-38; median and range]% of time; Ex = 2.4 [0-8.7]%) and galloped more (NoEx = 6 [2-8]%; Ex = 0 [0-4]%) than the Ex that were released daily, but walked less (NoEx = 17 [10-26]%; Ex = 35 [20-40]%) and grazed less (NoEx = 0%; Ex = 3 [0-12]%). Confined horses show rebound locomotion — that is, a compensatory increase — when released from confinement, indicating a response to exercise deprivation.

We previously reported that African (Loxodonta africana) and Asian (Elephas maximus) female elephants in European zoos have shorter adult lifespans than protected conspecifics in range countries. This effect was the cause of greatest concern in Asian elephants, and risk factors within this species included being zoo-born, transferred between zoos, and possibly removed early from the mother. Here, we investigate these risk factors further; assess fecundity and sustainability in European zoos; and propose testable hypotheses as to the causes of these animals’ problems. Although imported wild-born Asian elephants live longer than zoo-born conspecifics, being imported when juvenile or adult appears no more protective than being imported in infancy, suggesting that the benefits of being wild- rather than zoo-born are conferred early in life. Zoo-born Asian neonates are significantly heavier than those born to working animals in range countries, with a possible tendency to be fatter. In zoos, African elephants have tended to be removed from their mothers at older ages than young Asians, and were also transferred between zoos significantly less often: factors that could possibly underlie this species’ lower calf losses and improving adult survivorship in Europe. Both species have low fecundity in European zoos compared to in situ populations, and are not self-sustaining, declining at approximately 10% per annum if reliant on captive-bred females under historically prevailing conditions. Data from other species suggest that stress and/or obesity are parsimonious explanations for the suite of problems seen. We recommend specific screens for testing these hypotheses, and for potentially identifying vulnerable individuals within the extant zoo populations.

The EU-funded Animal Welfare Indicators (AWIN) research project (2011-2015) aimed to improve animal welfare through the development of practical on-farm animal welfare assessment protocols. The present study describes the application of the AWIN approach to the development of a welfare assessment protocol for horses (Equus caballus). Its development required the following steps: (i) selection of potential welfare indicators; (ii) bridging gaps in knowledge; (iii) consulting stakeholders; and (iv) testing a prototype protocol on-farm. Compared to existing welfare assessment protocols for other species, the AWIN welfare assessment protocol for horses introduces a number of innovative aspects, such as implementation of a two-level strategy focused on improving on-farm feasibility and the use of electronic tools to achieve standardised data collection and so promote rapid outcomes. Further refinement to the AWIN welfare assessment protocol for horses is needed in order to firstly gather data from a larger reference population and, secondly, enhance the welfare assessment protocol with reference to different horse housing and husbandry conditions.

The objective of the present study was to assess the sensitivity and feasibility of the Welfare Quality® (WQ) protocol for finishing pigs in 10 Spanish slaughterhouses. Sensitivity refers to the ability of the WQ assessment system to discriminate between slaughter conditions whilst feasibility denotes that the protocol is concise and easy to implement. On arrival at the plant, the incidence of dead, sick and panting animals was assessed in 1,002 (± 93) pigs per abattoir. During unloading, the percentage of pigs that slipped, fell, showed reluctance to move or turned back and were lame was also assessed. In the lairage pens, the stocking density and the percentage of pigs that were panting, shivering, and huddling was assessed in a total of 346 (± 81.0) pigs per abattoir. Stunning effectiveness, slaughter checks and skin lesions were also assessed in 60 animals per abattoir. For the majority of measures, any differences between slaughterhouses were found to be attributable to the installation itself and the management of the slaughterhouse, such as generalised fear, slipping and falling or stunning effectiveness, as opposed to measures taken to assess transport conditions or farm origin, such as lameness or sick and dead animals. The study protocol took 5.5 h for one observer to complete, in a slaughterhouse killing more than 550,000 pigs a year, although this time could increase dramatically in smaller abattoirs due to delays in the arrival of lorries. The protocol provides a general overview of the state of welfare of animals at the slaughterhouse and can readily identify specific problems in certain areas, such as stunning of animals.

Providing assurance of improvement in welfare outcomes is the desire of some UK certification schemes. However, this is only likely to be achieved if such schemes monitor welfare outcomes rather than rely on defining welfare resources that should be provided. The University of Bristol has developed protocols for the on-farm evaluation of animal-based welfare parameters for incorporation into certification schemes. The assessment system is designed to provide credible (repeatable, valid and feasible) evidence for assessment of compliance with welfare standards in organic and conventional farming systems. For issues identified as causing potential concern the assessor is encouraged to conduct further investigations of the relevant resource standards and, where appropriate, management requirements. Furthermore the assessment tool enables certification bodies and relevant third parties to monitor the ability of schemes to deliver good welfare outcomes. Finally, it provides a mechanism for farmers to review their own health and welfare performance. The practical application of these systems is now being pursued by some UK certification bodies.

Male laboratory rats (Rattus norvegicus; Wistar, Alderley Park) were housed as singletons or groups of three in units of two joined, but divided cages. Units were divided by different types of barrier that allowed different degrees of social contact across the barrier. Singletons were established either with another singleton as a neighbour on the other side of the barrier, or with a group of three as neighbours. Relative to group-housed animals, singly-housed rats showed reduced activity and a greater incidence of self-directed behaviours and behaviours apparently related to escape or seeking social information. Pathophysiological evidence was consistent with Baenninger's (1967) suggestion that tail manipulation in singletons is a surrogate social response, but was also consistent with an overall increase in self-directed activity, reflecting elasticity in time budgeting. Variation in the degree of increase in self-directed activity among singletons and the negative correlation between self-directed activity and organ pathology may have reflected differences in the ability of individuals to avoid an activity limbo. While reduced corticosterone concentration and organ pathology compared with grouped rats implied that separation may remove social stress, responses to contact with neighbours, and correlations between behaviours and organ pathology suggested that rats may actively seek social interaction. Broad differences in stress responses between single and grouped housing conditions may therefore be an inadequate yardstick to the animals’ welfare. However, exposure to neighbours reduced the aggressiveness of singly-housed males when they were eventually introduced into an unfamiliar group, suggesting that a degree of exposure to neighbours (separation, but not isolation) may have some welfare benefits for laboratory-housed rats, depending on procedures.

The focus of wildlife rehabilitation is the survival of the individual animal, often leading to rehabilitators being in conflict with government wildlife officials, who regulate the industry and whose focus is on the security of entire wildlife communities. In South Africa, wildlife rehabilitation has been the focus of recent attention from the general public, government and academics, due mostly to the development and adoption of norms and standards for the management of primates. Our study was initiated to provide the first survey of rehabilitation centres in South Africa. Questionnaires were returned by 65% known rehabilitation centres in South Africa, including all nine Provinces, through which several thousand injured, diseased and orphaned animals pass each year. It is clear there is a need for rehabilitation centres in South Africa. However, due to a lack of scientific research on the efficacy of rehabilitation methods for care and release, and minimal post-release monitoring, wildlife rehabilitation techniques and protocols have been based on work experience and subjective intuition. In conjunction with a lack of funds, there may be negative impacts on individual animal welfare and survival, as well as on conservation efforts for wildlife communities. Similar issues have been documented in other regions of the world. In the authors’ opinion, centralisation of wildlife rehabilitation to national or provincial government is a necessity. Furthermore, it is suggested that guidelines of minimum standards should be developed in consultation with experienced rehabilitators, veterinarians and conservation scientists; to be enforced by trained and dedicated conservation officials.

This study examined the effects of enriching the environment on the learning abilities of growing pigs. Eighty-four pigs were housed in either barren or enriched environments from birth to 14 weeks. The barren environments were defined as intensive housing and the enriched environments incorporated extra space, including areas which contained peat and straw in a rack. The learning abilities of pigs from both environments were tested at 15-17 weeks using an operant task which involved pigs learning to push a panel for a reward and a maze test which involved spatial learning. Pigs from enriched environments learned both the operant task and the maze task more rapidly than their counterparts from barren environments. These results suggest that the cognitive development of pigs may be impaired in intensive housing systems.

This paper discusses the potential for using observations of behaviour to recognise distress in sheep. The term distress is used to describe situations in which an animal is likely to be suffering, and is indicating this by overt behavioural signs. Literature on the behavioural responses of sheep to procedures that induce a physiological stress response is reviewed. This approach is based on human analogy and the assumption that physiological changes can be used to differentiate between stimuli that induce an emotional response in sheep and those that do not. The degree to which the behaviour of sheep in certain situations represents, at least in part, an expression of emotional behaviour, or whether it can be fully explained as a functional response to a specific situation, is a fundamental and unresolved question in ethological and psychological studies. Therefore, the validity of compiling a list of objective common behavioural indicators of distress in sheep will be contentious. However, it is important to be able to recognise and deal with suffering, and the use of behavioural methods for the identification of distress in sheep is a practical welfare issue. There is a need for further research to identify indicators of distress in sheep, but in the meantime it would be reasonable to make the judgement that, in some circumstances, sheep that are found to be vocalising, panting, and/or showing markedly increased locomotory activity could be experiencing distress.

The practice of farming bears for bile extraction is legal in China and involves an estimated 10,000 to 12,000 animals, primarily Asiatic black bears (Ursus thibetanus). This study outlines the compromises to health and welfare suffered by bears on bile farms and is based on the results of visits to more than 50 bear farms; 15 years’ worth of interviews with bear farmers, Chinese officials, practitioners of Traditional Chinese Medicine and vendors dealing in bear parts; and from the observation and care of approximately 250 bears that have been rescued from bile farms. Bears that have been rescued from farms display evidence of severe and chronic physical and psychological suffering. Medical complications arising from the husbandry and the gall bladder fistulation significantly, and often fatally, compromise bears’ health.

The process of driving improvement in animal welfare has three stages: 1) assessment of animal welfare, 2) identification of the risk factors potentially leading to a compromise in animal welfare, and 3) interventions, in response to the risk factors, to bring about improvement in animal welfare. This process is applicable to animals farmed in commercial situations and for individual animals kept or worked in isolated environments. An impressive weight of knowledge has accumulated in the science of animal welfare assessment and this needs to be counterbalanced by development of mechanisms to actively improve welfare. In the case of animal welfare, interventions have to motivate the animal owner or carer to make changes to their own behaviour on behalf of a third party; the animal. This is a different situation from that described in the human health literature where interventions encourage people to take steps to improve their own well-being, thus benefiting themselves directly. The development of strategies to improve animal welfare require a multi-disciplinary approach including social scientists, psychologists and economists, however, the skills of animal welfare scientists are essential to ensure that interventions truly achieve improvements in animal welfare.

Chronic stress occurs when animals are unable to deal with a persistent stressor with species-typical responses, or when several stressors are present concurrently. Chronic stress is most frequently considered in intensive systems, but it may also be a welfare concern for extensively managed species, such as the sheep. Here we review behavioural and physiological responses of sheep to experimentally induced chronic stressors to determine relevant indicators of chronic stress. Neuroendocrine responses to chronic stress are difficult to interpret because initial responses are followed by an apparent normalisation. Thus, cortisol or catecholamines may be at or below pre-stress levels during chronic stress, but this varies with different stressors. Chronic stress can also affect reproductive function, impair body and wool growth and meat quality, reduce immune function, and is associated with greater parasite burdens in sheep. Chronic stress induces alterations in behaviour patterns, particularly activity and feeding, and circadian rhythms of behaviour. Stereotypic behaviours, however, are infrequent in sheep and may occur only in experimental conditions of social isolation. Behavioural and physiological data suggest that rough handling and sheepdogs may be sources of chronic stress for sheep. Social subordination and weaning also act as chronic stressors, leading to higher parasitism in these animals and a greater response to additional stressors. Lameness and parasitism are associated with physiological and behavioural responses indicating that these are severe forms of chronic stress in sheep. It is unclear whether environmental stressors, such as weather and food availability, induce chronic stress in sheep. Under-nutrition may, however, be a welfare concern through its impact on lamb survival. The existence of many sources of chronic stress in the management of sheep suggests that the welfare of this species requires more attention than it has currently received.

A common and painful management practice undertaken on most dairy farms is dehorning young calves (also called ‘disbudding’ when done on calves less than about two months of age). Despite much evidence the practice is painful, and effective means available to mitigate this pain, it is frequently performed without pain relief. The overall aim of this study was to describe different stakeholder views on the use of pain mitigation for disbudding and dehorning. Using an interactive, online platform, we asked participants whether or not they believed that calves should be disbudded and dehorned with pain relief and to provide reasons to support their choice. Participant composition was as follows: dairy producer or other farm worker (10%); veterinarian or other professional working with the dairy industry (7%); student, teacher or researcher (16%); animal advocate (9%); and no involvement with the dairy industry (57%). Of 354 participants, 90% thought pain relief should be provided when disbudding and dehorning. This support was consistent across all demographic categories suggesting the industry practice of disbudding and dehorning without pain control is not consistent with normative beliefs. The most common themes in participants’ comments were: pain intensity and duration, concerns about drug use, cost, ease and practicality and availability of alternatives. Some of the participants’ reasoning corresponded well with existing scientific evidence, but other reasons illustrated important misconceptions, indicating an urgent need for educational efforts targeted at dairy producers and dairy industry professionals advising these producers.

This study explores the use of Free Choice Profiling (FCP) methodology for the qualitative behaviour assessment of emotional expression in the domestic dog (Canis familiaris). Qualitative behaviour assessment is based upon the integration of many pieces of information that in conventional quantitative approaches are recorded separately or not at all. Observers are asked to focus on an animal's interaction with its surrounding environment, and to describe the animal's expressive demeanor, or ‘body language’. A specific characteristic of FCP methodology is that it allows observers the freedom to devise their own descriptive terms, and then to use these personal terms to quantitatively score observed subjects. Application of FCP to qualitative behaviour assessment in animals was originally tested for pigs, and more recently for dairy cows, horses, and ponies. The goal of this study was to apply FCP to the domestic dog, and to investigate the inter-observer reliability of assessments of emotional expression in 10 individual Beagles by a group of 18 untrained observers. The data was analysed using Generalised Procrustes Analysis (GPA), a multivariate statistical technique associated with FCP. The observers achieved highly-significant agreement in their assessments of the dogs’ expressions, thereby establishing the applicability of this methodology for the first time in the domestic dog.

Each year, nearly 4 million dogs will enter one of over 13,000 animal shelters operating in the United States. We review programmes implemented at shelters aimed at increasing the likelihood of adoption. The morphology of shelter dogs plays a large role in in-kennel adopter selection, but their behaviour is also influential in out-of-kennel adopter interactions. Previous studies suggest that dogs have the ability to readily learn new behaviours at the shelter, and programmes designed to improve behaviour of the dogs can increase adoption rates. Whilst human interaction has been well-established to improve behavioural and physiological outcomes of dogs living in shelters, analysis of the effects of sensory, environmental, and social-conspecific enrichment has not resulted in clear conclusions. We also review the literature on the relinquishment of owned dogs and return rates of previously adopted dogs. Whilst owner- and dog-related risks to relinquishment are discussed, we show that there is a notable lack of research into programmes that address issues that may prevent the initial surrender of dogs to shelters, or that could prevent re-relinquishment. It is likely that factors, unrelated to the dog, play a larger role than previously believed. Suggestions for further research include multi-site studies, investigations into the efficacy of in-shelter enrichment programmes, predictive validity of behavioural assessments, understanding of adopter behaviour at the shelter, and programmes within the community focused on keeping dogs in their homes.

While the international zoological community is committed to enhancing the welfare of individual animals, researchers have yet to take full advantage of the tools available for non-invasively tracking behavioural and physiological indicators of welfare. We review technology currently being applied in studies of zoo, farm and laboratory animals to regularly monitor welfare status, as well as to evaluate responses to particular stimuli and situations. In terms of behavioural measures, we focus on automated assessments that offer insight into how animals — even those that are nocturnal or elusive — behave when humans are not present. Specifically, we provide an overview of how animal-attached technology (accelerometers, global positioning systems, radio frequency identification systems) can be implemented to generate activity budgets, examine use of space, conduct gait assessments, determine rates of movement and study social dynamics. We also emphasise the value of bioacoustics, as the rate and acoustic structure of certain vocalisations may vary across contexts and reflect an animal's internal state. While it can be challenging to identify non-invasive methods for investigating physiological welfare indicators, we discuss approaches (thermography, tracking measures of heart rate) that may be especially useful for monitoring affective states and psychophysiological functioning. Finally, we make a concerted effort to highlight tools that allow welfare scientists to consider measures of positive welfare. Ultimately, zoos can ensure that each animal has the opportunity to thrive by employing technology to create baseline behavioural and physiological profiles, conduct ongoing monitoring schemes and assess responses to specific conditions, events and stimuli.

The United States has traditionally lagged behind Europe in the adoption of voluntary or legislated standards for the care and treatment of animals on farms. US federal legislation of farm animal practices is minimal, confined to aspects of livestock transport and slaughter. Although some of the livestock and poultry producer (commodity) groups wrote guidelines, codes of practice, or statements regarding the humane treatment of animals in the 1980s, these were usually very general statements of current industry practice, developed with little consultation with independent experts and involving no mechanism for encouraging or ensuring compliance by producers. However, this has changed dramatically in the last few years, with an increasing trend among US retailers to require their suppliers to adopt minimum animal welfare standards. The major chain restaurants and supermarkets are working through their trade organisations, the National Council of Chain Restaurants and the Food Marketing Institute respectively, and with the commodity groups, to develop a uniform set of standards and a national auditing program. Standards and auditing programs have already been approved for dairy cattle, laying hens and meat chickens, and for slaughter, including ritual slaughter (kosher and halal). The process of setting auditable standards is complicated by the lack of legislative underpinning, the scope of the auditing that will be required because of US farm sizes and the large distances between farms, and the varying levels of expertise of potential auditors. For these reasons, ‘engineering-based’ auditing criteria that are relatively easy to measure and to standardise are more common. There are both strengths and potential weaknesses of retail-driven rather than legislatively driven animal welfare standards. Regardless, the recent changes in the US possibly pave the way for increasing dialogue between Europe and the US on farm animal welfare issues.