Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-26T05:57:33.092Z Has data issue: false hasContentIssue false
  • English
  • Français

Impact of trapping and handling on Leukocyte Coping Capacity in bank voles (Clethrionomys glareolus) and wood mice (Apodemus sylvaticus)

Published online by Cambridge University Press:  01 January 2023

M Gelling ,
GW McLaren ,
F Mathews ,
R Mian  and
DW Macdonald
M Gelling*
Affiliation:
Wildlife Conservation Research Unit, Department of Zoology, University of Oxford, Tubney House, Abingdon Road, Tubney, Oxon OX13 5QL, UK
GW McLaren
Affiliation:
Wildlife Conservation Research Unit, Department of Zoology, University of Oxford, Tubney House, Abingdon Road, Tubney, Oxon OX13 5QL, UK Magnox North, Building B12, Berkeley Centre, Berkeley, Gloucestershire GL13 9PB, UK
F Mathews
Affiliation:
Wildlife Conservation Research Unit, Department of Zoology, University of Oxford, Tubney House, Abingdon Road, Tubney, Oxon OX13 5QL, UK School of Biosciences, University of Exeter, Hatherley Laboratories, Prince of Wales Road, Exeter EX4 4PS, UK
R Mian
Affiliation:
Oxford MediStress, Suite 125, 27 Colmore Row, Birmingham B3 2EW, UK
DW Macdonald
Affiliation:
Wildlife Conservation Research Unit, Department of Zoology, University of Oxford, Tubney House, Abingdon Road, Tubney, Oxon OX13 5QL, UK
*
* Contact for correspondence and requests for reprints: [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Small mammals are routinely live-trapped and subsequently handled for a range of ecological and behavioural studies. Despite the techniques commonly employed being potentially stressful for the individual animals involved, it has hitherto been difficult to quantify the physiological impact. Here, we report on the first instance of using the Leukocyte Coping Capacity technique (LCC) in bank voles (Clethrionomys glareolus) and wood mice (Apodemus sylvaticus) to investigate the physiological impact of routine trapping and handling techniques. Twenty microlitres of blood were obtained from 40 animals, of which 25 were handled following standard protocols and 15 were not. We found that even a short period of acute stress is sufficient to trigger an immune response which was measurable using the LCC technique. These results further validate the use of the LCC technique for measuring the physiological impact of standard trapping and handling treatments on wild mammals.

Keywords

animal welfarehandlingLeukocyte Coping Capacitysmall mammalsstresstrapping
Type
Research Article
Information
Animal Welfare , Volume 18 , Issue 1 , February 2009 , pp. 1 - 7
Copyright
© 2009 Universities Federation for Animal Welfare

References

Alberts, B, Bray, D, Lewis, J, Roff, M, Roberts, K and Watson, JD 1994 Molecular Biology of the Cell. Garland Publishing Inc: New York, USAGoogle Scholar
Alonso-Alvarez, C, Bertrand, S, Devevey, G, Prost, J, Faivre, B and Sorci, G 2004 Increased susceptibility to oxidative stress as a proximate cost of reproduction. Ecology Letters 7: 363–368CrossRefGoogle Scholar
Avitsur, R, Stark, JL, Dhabhar, FS, Kramer, KA and Sheridan, JF 2003 Social experience alters the response to social stress in mice. Brain, Behaviour & Immunity 17: 426–437CrossRefGoogle ScholarPubMed
Bachman, GC 2003 Food supplements modulate changes in leukocyte numbers in breeding male ground squirrels. Journal of Experimental Biology 206: 2373–2380CrossRefGoogle Scholar
Braude, S, Tang-Martinez, Z and Taylor, GT 1998 Stress, testosterone, and the immunoredistribution hypothesis. Behaviour & Ecology 10: 345–350CrossRefGoogle Scholar
Castell, LM and Newsholme, EA 2001 The relation between glutamine and immunodepression observed in exercise. Amino Acids 20: 49–61CrossRefGoogle ScholarPubMed
Castell, LM 2002 Can glutamine modify the apparent immunodepression observed after prolonged, exhaustive exercise? Nutrition 18: 371–375CrossRefGoogle ScholarPubMed
Chen, CS 2002 Phorbol ester induces elevated oxidative activity and alkalization in a subset of lysosomes. BMC Cell Biology 3: 34CrossRefGoogle Scholar
Dhabhar, FS and McEwen, BS 1997 Acute stress enhances while chronic stress suppresses cell-mediated immunity in vivo: A potential role for leukocyte trafficking. Brain Behavior & Immunity 11(4): 286–306CrossRefGoogle ScholarPubMed
Ellard, DR, Castle, PC and Mian, R 2001 The effect of a short-term mental stressor on neutrophil activation. International Journal of Psychophysiology 41: 93–100CrossRefGoogle ScholarPubMed
Fekete, SG and Kellems, RO 2007 Interrelationship of feeding with immunity and parasitic infection: a review. Veterinarni Medicina 52: 131–143CrossRefGoogle Scholar
Fletcher, QE and Boonstra, R 2006 Impact of live trapping on the stress response of the meadow vole (Microtus pennsylvanicus). Journal of Zoology 270: 473–378CrossRefGoogle Scholar
Gurnell, J and Flowerdew, JR 1994 Live Trapping of Small Mammals: A Practical Guide. The Mammal Society: London, UKGoogle Scholar
Hayes, JP and Shonkwiler, JS 2001 Morphometric indicators of body condition: worthwhile or wishful thinking? In: Speakman, JR (ed) Body Composition Analysis of Animals: A Handbook of Non-Destructive Methods. Cambridge University Press: Cambridge, UKGoogle Scholar
Honess, PE, Marin, C, Brown, AP and Wolfensohn, SE 2005 Assessment of stress in non-human primates: application of the neutrophil activation test. Animal Welfare 14: 291–295Google Scholar
Hu, T, Bei, L, Qian, Z and Shen, X 1999 Intracellular free calcium regulates the onset of the respiratory burst of human neutrophils activated by Phorbol Myristate Acetate. Cell Signal 11: 355–360CrossRefGoogle ScholarPubMed
Kruidenier, L and Verspaget, HW 2002 Oxidative stress as a pathogenic factor in inflammatory bowel disease — radicals or ridiculous? Alimentary Pharmacology & Therapeutics 16: 1997–2015CrossRefGoogle ScholarPubMed
Lin, X, Tan, WC and Candlish, JK 1995 Effect of glucose on the respiratory burst of circulating neutrophils from asthmatics. Experimental Molecular Pathology 62: 1–11CrossRefGoogle ScholarPubMed
Mathews, F, Honess, P and Wolfensohn, S 2002 Use of inhalation anaesthesia for wild mammals in the field. Veterinary Record 150: 785–787CrossRefGoogle ScholarPubMed
McEwen, BS 1998 Stress, adaptation and disease — allostasis and allostatic load. Annals of the New York Academy of Sciences 840: 33–44CrossRefGoogle ScholarPubMed
McLaren, GW, Macdonald, DW, Georgiou, C, Mathews, F, Newman, C and Mian, R 2003 Leukocyte coping capacity: a novel technique for measuring the stress response in vertebrates. Experimental Physiology 88: 541–546CrossRefGoogle ScholarPubMed
McLaren, GW, Mathews, F, Fell, R, Gelling, M and Macdonald, DW 2004 Body weight change as a measure of stress: a practical test. Animal Welfare 13: 337–341Google Scholar
McLaren, GW, Bonacic, C and Rowan, A 2007 Animal welfare and conservation: measuring stress in the wild. In: Macdonald, DW and Service, K (eds) Key Topics in Conservation Biology. Blackwell Publishing Ltd: Oxford, UKGoogle Scholar
Mian, R, Shelton-Rayner, G, Harkin, B and Williams, P 2003 Observing a fictitious stressful event: haematological changes including circulating leukocyte activation. Stress 6: 41–47CrossRefGoogle ScholarPubMed
Mian, R, McLaren, G and Macdonald, DW 2005 Of stress, mice and men: a radical approach to old problems. In: Oxington, K (ed) Stress and Health: New Research. Nova Science Publications: New York, USAGoogle Scholar
Millspaugh, JJ, Coleman, MA, Bauman, BJ, Raedeke, KJ and Brundige, GC 2000 Serum profiles of American elk at the time of handling for three capture methods. Canadian Field Naturalist 114: 196–200Google Scholar
Montes, I, McLaren, GW, Macdonald, DW and Mian, R 2003 The effects of acute stress on leukocyte activation. Journal of Physiology 54: 88Google Scholar
Montes, I, McLaren, GW, Macdonald, DW and Mian, R 2004 The effect of transport stress on neutrophil activation in wild badgers (Meles meles). Animal Welfare 13: 355–359Google Scholar
Moorhouse, TP, Gelling, M, McLaren, GW, Mian, R and Macdonald, DW 2007 Physiological consequences of captive conditions in water voles (Arvicola terrestris). Journal of Zoology 271: 19–26CrossRefGoogle Scholar
Pithon-Curi, TC, Levada, AC, Lopes, LR, Doi, SQ and Curi, R 2002 Glutamine plays a role in superoxide production and the expression of p47 phox p22 phox and gp91 phox in rat neutrophils. Clinical Science 103: 403–408CrossRefGoogle Scholar
Pocock, MJO and Jennings, N 2006 Use of hair tubes to survey for shrews: new methods for identification and quantification of abundance. Mammal Review 36: 299–308CrossRefGoogle Scholar
Prout, DM and King, CM 2006 The effect of handling under anaesthetic on the recapture rate of wild ship rats (Rattus rattus). Animal Welfare 15: 63–66Google Scholar
Råberg, L, Grahn, M, Hasselquist, D and Svensson, E 1998 On the adaptive significance of stress induced immunosuppression. Proceedings of the Royal Society of London B265: 1637–1641CrossRefGoogle Scholar
Roberts, ML, Buchanan, KL, Hasselquist, D and Evans, MR 2007 Effects of testosterone and costicosterone on immunocompetence in the zebra finch. Hormones and Behavior 51: 126–134CrossRefGoogle Scholar
Romano, TA, Keogh, MJ, Kelly, C, Feng, P, Schlundt, CE, Carder, DA and Finneran, JJ 2004 Anthropogenic sound and marine mammal health: measures of the nervous and immune systems before and after intense sound exposure. Canadian Journal of Fisheries and Aquatic Sciences 61: 1124–1134CrossRefGoogle Scholar
Sanidas, D, Garnham, A and Mian, R 2000 Activation of human leukocytes by hypoxia. Experimental Physiology 85: 263–266Google ScholarPubMed
Sanidas, D, Garnham, A and Mian, R 2002 Hypoxia-induced chemiluminescence in human leukocytes; the role of calcium. European Journal of Pharmacology 453: 183–187CrossRefGoogle Scholar
Uruňuela, A, Sevillano, S, de la Mano, AM, Manso, MA, Orfao, A and de Dios, I 2002 Time-course of oxygen free radical production in acinar cells during acute pancreatitis induced by pancreatic duct obstruction. Biochimica et Biophysica Acta 1588: 159–164CrossRefGoogle ScholarPubMed
Village, A and Myhill, D 1990 Estimating abundance for predator studies - snap-trapping versus sign indexes. Journal of Zoology 222: 68I–689CrossRefGoogle Scholar
Whitehead, TP, Thorpe, GHG and Maxwell, SRJ 1992 Enhanced chemiluminescent assay for antioxidant capacity in biological fluids. Analytica Chimica Acta, 266: 265–277CrossRefGoogle Scholar