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Changes in ear-pinna temperature as a useful measure of stress in sheep (Ovis aries)

Published online by Cambridge University Press:  11 January 2023

TE Lowe ,
CJ Cook ,
JR Ingram  and
PJ Harris
TE Lowe*
Affiliation:
Bioengineering, HortResearch, Private Bag 3123, Hamilton, New Zealand
CJ Cook
Affiliation:
Bioengineering, HortResearch, Private Bag 3123, Hamilton, New Zealand
JR Ingram
Affiliation:
Bioengineering, HortResearch, Private Bag 3123, Hamilton, New Zealand School of Neuroscience and Psychiatry, University of Newcastle, Newcastle-upon-Tyne NE14LP, UK
PJ Harris
Affiliation:
Bioengineering, HortResearch, Private Bag 3123, Hamilton, New Zealand Thales Research, Worton Drive, Worton Grange, Reading RG2 0SB, UK
*
* Contact for correspondence and requests for reprints: [email protected]
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Abstract

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Activation of the sympathetic nervous system, with associated increases in heart rate and the redistribution of blood in preparation for ‘fight or flight’, is an integral part of the ‘defence reaction’. In sheep, the defence reaction involves vasoconstriction in the ear-pinna. If decreases in ear-pinna temperature (Tp) can be used to indicate vasoconstriction, then it may be possible to use changes in Tp as a measure of the defence reaction. Ewe lambs were exposed to stressors including mustering into pens, moving between pens, isolation from conspecifics, and prolonged periods of exercise. Measurements of heart rate (HR), Tp, vaginal temperature (Tv), and salivary cortisol and urinary catecholamine concentrations were used to assess stress responses. A repeatable pattern of changes in HR, Tp and Tv was observed in response to stressors. Short-term disturbances resulted in increased HR, reduced Tp, and increased Tv. More sustained disturbances — for example, prolonged periods of exercise — resulted in a sustained elevation in HR, a sustained decrease in Tp, and a sustained elevation in Tv. The highest levels of cortisol and catecholamines were associated with the treatments that resulted in the longest periods of decreased Tp. We infer that changes in Tp occur largely in response to changes in sympathetic nervous activity, and that the potential exists to measure elements of stress responses by monitoring Tp in freely behaving animals. This is a minimally invasive measure that allows the monitoring of modest numbers of animals over prolonged periods with minimal handling.

Keywords

animal welfaredefence reactionear-pinnasheepstresstemperature logger
Type
Research Article
Information
Animal Welfare , Volume 14 , Issue 1 , February 2005 , pp. 35 - 42
Copyright
© 2005 Universities Federation for Animal Welfare

References

Abrahams, VC, Hilton, SM and Zbrozyna, A 1960 Active muscle vasodilation produced by stimulation of the brain stem: its significance in the defence reaction. Journal of Physiology 154: 491–513CrossRefGoogle ScholarPubMed
Abrahams, VC, Hilton, SM and Zbrozyna, A 1962 Sensory connexions to the hypothalamus and midbrain, and their role in the reflex activation of the defence reaction. Journal of Physiology 164: 1–16CrossRefGoogle Scholar
Bell, AW, Hales, JRS, King, RB and Fawcett, AA 1983 Influence of heat stress on exercise-induced changes in regional blood flow in sheep. Journal of Applied Physiology 55(6): 1916–1923CrossRefGoogle ScholarPubMed
Blessing, WW 2003 Lower brainstem pathways regulating sympathetically mediated changes in cutaneous blood flow. Cellular and Molecular Neurobiology 23(4–5): 527–538CrossRefGoogle ScholarPubMed
Bligh, J and Milton, AS 1973 The thermoregulatory effects of prostaglandin E1 when infused into the lateral cerebral ventricle of Welsh Mountain sheep at different ambient temperatures. Journal of Physiology 229: 30–31Google ScholarPubMed
Cabanac, M and Seika, A 2000 Fever and tachycardia in a bird (Gallus domesticus) after simple handling. Physiology & Behavior 69: 541–545CrossRefGoogle Scholar
Canon, WB 1929 Bodily Changes in Pain, Hunger, Fear and Rage (Edition 2). Appleton: New York, USAGoogle Scholar
Hales, JRS 1986 Adrenergic mechanisms in the circulatory responses to heat stress. In: Henriksen, O and Lassen, NA (eds) The Sympathoadrenal System. Munksgaard: Copenhagen, DenmarkGoogle Scholar
Harris, PJ, Henderson, JD and Schaare, PN 1998 An ambulatory physiological monitor for extended use in animal welfare studies. Proceedings of the 20th Annual International Conference of the IEEE Engineering in Medicine and Biology Society 20(4): 1917–1920Google Scholar
He, H, Stein, MC, Christman, B and Wood, AJJ 1997 Determination of catecholamines in sheep plasma by high-performance liquid chromatography with electrochemical detection: comparison of deoxyepinephrine and 3,4-dihydroxybenzylamine as internal standard. Journal of Chromatography B 701: 115–119CrossRefGoogle ScholarPubMed
Hilton, SM 1982 The defence–arousal system and its relevance for circulatory and respiratory control. Journal of Experimental Biology 100: 159–174CrossRefGoogle ScholarPubMed
Ingram, JR, Cook, CJ and Harris, PJ 2002 The effect of transport on core and peripheral body temperatures and heart rate of sheep. Animal Welfare 11: 103–113Google Scholar
Kluger, MJ, O'Reilly, B, Shope, TR and Vander, AJ 1987 Further evidence that stress hyperthermia is a fever. Physiology & Behavior 39: 763–766CrossRefGoogle ScholarPubMed
Li, ZY, Koman, LA, Rosencrance, E, Smith, BP and Smith, TL 1998 Endogenous nitric oxide influences arteriovenous anastomosis adrenergic tone in the conscious rabbit ear. Journal of Cardiovascular Pharmacology 32(3): 349–356CrossRefGoogle ScholarPubMed
Lowe, TE, Devine, CE, Wells, RW and Lynch, LL 2004 The relationship between postmortem urinary catecholamines, meat ultimate pH, and shear force in bulls and cows. Meat Science 67: 251–260CrossRefGoogle ScholarPubMed
Moltz, H 1993 Fever: causes and consequences. Neuroscience and Biobehavioral Reviews 17: 237–269CrossRefGoogle ScholarPubMed
Parrott, RF and Lloyd, DM 1995 Restraint, but not frustration, induces prostaglandin-mediated hyperthermia in pigs. Physiology & Behavior 57: 1051–1055CrossRefGoogle Scholar
Parrott, RF, Lloyd, DM and Brown, D 1999 Transport stress and exercise hyperthermia recorded in sheep by radiotelemetry. Animal Welfare 8: 27–34Google Scholar
Sawka, MN and Wenger, CB 1988 Physiological responses to acute exercise-heat stress. In: Pandoaf, KB, Sawka, MN and Gonzalez, RR (eds) Human Performance Physiology and Environment Medicine at Terrestrial Extremes. Benchmark Press: Indianapolis, USAGoogle Scholar
Tarrant, PV 1988 Animal behaviour and environment in the dark-cutting condition. In: Fabiansson SU, Shorthose WR and Warner RD (eds) Dark-Cutting in Cattle and Sheep. Proceedings of an Australian workshop. Australian Meat and Live-stock Research and Development Corporation: Sydney, AustraliaGoogle Scholar
Thienpont, LM, Van Landuyt, KG, Stöckl, D, Saeyens, W, Keukeleire, DD and De Leenheer, AP 1995 Evaluation of 2-iminoimidazolidin-4-one and thymine as respective internal standards for normal-phase and reversed-phase liquid chromatographic determination of creatinine in human serum. Journal of Chromatography B 665: 63–69CrossRefGoogle ScholarPubMed
Warriss, PD 1990 The handling of cattle pre-slaughter and its effects on carcass and meat quality. Applied Animal Behaviour Science 28: 171–186CrossRefGoogle Scholar
Yu, YH and Blessing, WW 1997 Cutaneous vasoconstriction in conscious rabbits during alerting responses detected by hippocampal θ-rhythm. American Journal of Physiology 272: R208–R216Google Scholar
Yu, YH and Blessing, WW 2001 Neurons in amygdala mediate ear pinna vasoconstriction elicited by unconditioned salient stimuli in conscious rabbits. Autonomic Neuroscience 87(2–3): 236–242CrossRefGoogle ScholarPubMed