Problems with getting up can affect welfare, therefore a simple method for use in assessing rising behaviour was evaluated. Sixty-one Danish Friesian cows housed in two identical tie-stall barns were used. The cows were in their first (no. = 30), second (no. = 16) or third lactation (no. = 15). There were 19 cows in early lactation (<100 days in milk), 18 late lactation cows (>200 days in milk) and 24 dry cows, divided between the age groups. Rising was scored at three times of day for five consecutive days. Two observers scored the cows at 11:30 h and one of these observers scored them at 15:00 and 17:30 h. Cows were encouraged to rise using increasing levels of encouragement but the minimum possible force and were scored for rising (between 1 - normal rising sequence, smooth movement and 5 - rising front first) and the level of encouragement required. The behaviour of each cow was recorded on video for 21·5 h. Total lying time; lying frequency; maximum lying bout length; time to lie down; time for preparatory phase of lying; time to rise, and time for final phase of rising were recorded from the videos and video records of rising were scored. The rising score was repeatable and was unaffected by the different scoring conditions tested (presence of observer, day of scoring, time of day, level of encouragement). Stage of lactation affected total lying time, number of lying bouts, maximum bout length and rising behaviour, while lactation number only had a minor effect on lying behaviour. The proposed score for rising reliably reflected whether the cows in tie-stalls had difficulty rising when at least three observations were included. The proportion of cows in different stages of lactation and of different parities should be included in any assessment of rising behaviour, since stage of lactation and parity significantly affected rising behaviour.

Modern, intensive fox breeding implies a human-animal relationship but human contact causes stress in farm-bred blue foxes. Handling is a common part of routine farming practices, particularly during the breeding season. The purpose of this study was to determine how handling influences certain morphometric and hormonal parameters of adrenocortical and ovarian function and fertility in pregnant blue foxes and whether these effects are mediated through an increase in plasma concentrations of cortisol. Blue fox females were subjected to a 1-min daily-handling treatment in the last trimester of pregnancy (term = 52 days). Plasma concentrations of ACTH, cortisol, progesterone, oestradiol and testosterone, as well as the in vitro adrenal and gonadal production of steroids were measured by radio-immuno assay in control (C, no. = 6) and handled (H, no. = 7) vixens on days 47 to 48 of pregnancy. In addition, the number of viable and dead foetuses, body, adrenal and ovarian weights in pregnant foxes and body weights of their viable foetuses were recorded. Handling increased plasma concentration of cortisol (C: 6·8 (s.e. 1·6) ng/ml v. H: 22·8 (s.e. 5·9) ng/ml, P < 0·05) as well as in vitro adrenal production of cortisol. In addition, handling decreased the body (C: 7·40 (s.e. 0·48) kg v. H: 6·24 (s.e. 0·13) kg, P < 0·05) and ovarian (C: 3·45 (s.e. 0·26) g v. H: 2·73 (s.e. 0·17) g, P < 0·05) weights of pregnant vixens and also the body weight of viable foetuses (C: 59·1 (s.e. 0·9) g, no. = 73 v. H: 50·6 (s.e. 1·2) g, no. = 58, P < 0·01). Handling did not cause any changes in adrenal weight, plasma concentrations of progesterone and oestradiol or in vitro ovarian steroid production. It is concluded that persistent handling of pregnant blue foxes is a mild stressor, which results in an increase in plasma concentration and adrenal production of cortisol and reduces maternal and foetal body weights.

Two experiments were conducted to determine if type of stocking system influenced the intake and live-weight gain (LWG) of cattle co-grazed with sheep on sown ryegrass/white clover pasture.

In experiment 1 (134 days), yearling heifers (no. = 9) plus ewe hoggets (no. = 27) were co-grazed (1: 1 M0·75) using continuous (C-CS) or rotational (R-CS) stocking. In experiment 2 (126 days), a cattle alone (no. = 9) treatment was included under each stocking system (C-C and R-C). Initial live weight of heifers was 266 (s.e. 4·5) and 232 (s.e. 4·4) kg and that of hoggets was 54 (s.e. 0·9) and 47 (s.e. 0·7) kg in experiments 1 and 2, respectively. In both experiments, the area offered daily to R-CS group was manipulated to promote a weekly live-weight change in sheep similar to that on the C-CS treatment. C-C cattle in experiment 2 were grazed at similar sward surface height (SSH) to C-CS, and R-C cattle at similar pre- and post-grazing SSH to R-CS. SSH was measured daily on all treatments and regulated on the continuously stocked treatments by addition and removal of non-experimental animals. Organic matter intake (OMI) was determined from the ratio of n-alkanes in faeces and herbage. Animals were weighed weekly.

Mean SSH on continuously stocked pastures was 5·10 (s.e. 0·03) and 4·26 (s.e. 0·02) cm for C-CS in experiments 1 and 2, respectively, and 4·27 (s.e. 0·02) cm for C-C in experiment 2. The mean pre- and post-grazing SSH for R-CS was 15·9 (s.e. 0·12) and 5·60 (s.e. 0·07) cm, respectively in experiment 1, and 15·2 (s.e. 0·08) and 4·82 (s.e. 0·03) cm, respectively in experiment 2. On R-C swards pre- and post-grazing SSH was 14·9 (s.e. 0·08) and 4·87 (s.e. 0·03) cm, respectively.

In experiment 1, cattle continuously co-grazed with sheep grew significantly more slowly than those rotationally co-grazed with sheep (804 (s.e. 41·6) v. 1039 (s.e. 47·7) g/day, P < 0·01). Sheep LWG did not differ between stocking treatments (150 v. 138 g, P > 0·05 for C-CS and R-CS respectively). These findings were confirmed in the second experiment in which C-CS cattle only grew at 0·69 of the daily LWG achieved by R-CS cattle (706 v. 1028, (s.e. 72) g/day; P < 0·05) at similar sheep LWG (155 v. 147, (s.e. 6·5) g/day respectively). LWG of C-C and R-C cattle was similar (916 v. 1022, (s.e. 72) g/day; P > 0·05). LWG per ha in both experiments was higher on R-CS than on C-CS treatments, and on cattle alone than on CS treatments. Treatment effects on OMI and final fasted live weight were similar in pattern to LWG.

It is suggested that the observed disadvantage to cattle when co-grazed with sheep under continuous stocking and the lack of effect when rotationally co-grazed reflected a difference in the two stocking systems in providing opportunities for complementary/competitive use of pasture resources.

Under field conditions, ewe behaviour was quantified by maternal behaviour score (MBS)-a six-point scale which assesses ewe flight distance when lambs are handled for the first time, within 24 h of birth (adapted from O’Connor et al., 1985). MBS was measured on 847 Scottish Blackface ewes on two experimental hill farms, over a maximum of four parities. Live weights of lambs born to these ewes were measured at birth, marking (average age of 42 days) and weaning (average age of 120 days).

MBS was significantly higher ( P < 0·05) for ewes with more lambing experience (multiparous ewes), for older ewes and for twin-bearing ewes compared with ewes with single lambs. MBS was under genetic control ( h2 = 0·13, permanent environmental effect = 0·19, when analysed as the same trait across parities) and the repeatability was 0·32.

MBS had no significant effects on weight gain of lambs. However, a moderate, positive genetic correlation was estimated between MBS and the average weight gained by lambs from birth to marking ( rg = 0·4, s.e. 0·22), although the genetic correlation estimated between MBS and weight gained to weaning was close to zero ( rg = 0·02, s.e. 0·16). Ewes with a MBS of 1 (ewe flees and does not return to her lambs) had significantly more lambs dying before marking and weaning than ewes with a higher maternal behaviour score. Selection to reduce the proportion of ewes in this category may therefore be beneficial.

Two experiments were carried out with pregnant (experiment 1) and lactating ewes (experiment 2), to compare dry-matter (DM) intake, and total tract apparent digestibility, digesta kinetics and weight of digestive tract contents of Lacaune and Manchega sheep, with the aim of explaining possible differences between the breeds in voluntary food intake. In experiment 1, 20 3-year-old single-bearing pregnant ewes, 10 Manchega and 10 Lacaune, were permanently housed for the last 10 weeks of pregnancy. The diet used consisted of lucerne hay, offered ad libitum, supplemented with 0·3 kg/day of concentrate. DM and digestible DM intake per kg M0·75 were higher (P < 0·01) in Lacaune than in Manchega sheep. Breed did not affect total tract apparent digestibility, fractional rates of passage, transit time, total mean retention time, or weight of digestive tract contents. Changes in body weight and body condition score were similar between breeds. In contrast, Lacaune lambs tended (P = 0·09) to weigh less than Manchega lambs, suggesting genetic differences in the energy utilization between breeds in late pregnancy. Higher DM intake observed in Lacaune sheep may have been related to a higher energy demand for mammary development. In experiment 2, 32 3-year-old lactating multiparous ewes, 16 Manchega and 16 Lacaune, were permanently housed during the first 12 weeks of lactation. The experimental diet used was based on a mixture of maize silage and dehydrated lucerne (10: 1, fresh weight basis), offered ad libitum, and supplemented with 0·8 kg/day of concentrate. Milk, fat and protein yield as well as DM and digestible DM intake in Lacaune ewes was higher (P < 0·01) than in Manchega ewes. DM intake was constant in Lacaune sheep with advancing lactation, while in Manchega sheep DM intake decreased. Throughout this period Lacaune ewes lost 0·5 kg of body weight while Manchega gained 4·4 kg. Breed did not affect either apparent digestibility of DM, organic matter and neutral-detergent fibre, or fractional rates of passage, transit time and total mean retention time. The weight of total tract digestive contents was greater (P < 0·05) in Lacaune than in Manchega sheep, particularly in the reticulo-rumen. Results suggest that the scheme of selection in Lacaune dairy sheep has increased milk yield together with voluntary food intake, the latter being associated with an increase in the rumen fill capacity. The higher milk yield of Lacaune ewes cannot be attributed to the higher DM intake only; other factors, i.e. mobilization of fat reserves, are required to support this higher milk output.