Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-26T10:39:30.826Z Has data issue: false hasContentIssue false
  • English
  • Français

Urinary excretions of purine derivatives and nitrogen in sheep given straw supplemented with different sources of carbohydrates

Published online by Cambridge University Press:  02 September 2010

J. Balcells ,
M. Fondevila ,
J. A. Guada ,
C. Castrillo  and
J. C. E. Surra
J. Balcells
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Miguel Servel 177, 50013 Zaragoza, Spain
M. Fondevila
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Miguel Servel 177, 50013 Zaragoza, Spain
J. A. Guada
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Miguel Servel 177, 50013 Zaragoza, Spain
C. Castrillo
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Miguel Servel 177, 50013 Zaragoza, Spain
J. C. E. Surra
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Miguel Servel 177, 50013 Zaragoza, Spain
Get access

Abstract

Estimations of purine derivatives excretion and urinary-nitrogen loss were used to test the response of rumen fermentation to supplementation of straw with different sources of carbohydrate. Two groups of Rasa Aragonesa ewes (44 (s.e. 0·75) kg live weight were given ad libitum basal diets of either ammonia-treated (ATS) or urea-supplemented (USS) barley straw, with 12 animals per basal diet group. Three supplements, barley grain, sugar-beet pulp or grass hay, respectively, were given to each basal diet group, giving a total of six dietary treatments with four animals per treatment group. Four levels of supplementation were studied (150, 300, 450 and 600 g air dry matter per day), one in each of four experimental periods. Each 45-day experimental period comprised 38 days of adjustment followed by a 7-day measurement period. Digestible organic matter (DOM) intake was higher in animals receiving ATS than in animals receiving USS (504 v. 474 (s.e. 21·1) g/day, P < 0·005) and higher in animals receiving barley grain and sugar-beet pulp than in those receiving grass hay (512 and 496 v. 370 (s.e. 25·9) g/day, P < 0·005). DOM intake also increased with the level of supplementation and this increase was greater with barley grain (504 to 634 and 314 to 554 g/day for ATS and USS) and sugar-beet pulp (440 to 582 and 315 to 522 g/day) than with grass hay (430 to 407 and 267 to 370 for ATS and USS). Urinary excretions of hypoxanthine, xanthine and uric acid were not affected by the experimental treatment whereas allantoin excretion (y, mmol) increased in response to DOM intake (x, kg) (y = 13·72 × − 0·26; r = 0·79; P < 0·001; no. = 96). The response in allantoin excretion was mainly explained by the increase in DOM intake. However when data were expressed per unit of DOM intake significant differences were still evident. Allantoin/DOM intake (mmol·kg) ratio and calculated microbial nitrogen (g·kg DOM intake) supply were lower with USS diets and sugar-beet pulp supplemented diets (P< 0·05) and increased significantly with level of supplementation (P < 0·001).

Keywords

allantoincarbohydratessheepurine
Type
Research Article
Information
Animal Production , Volume 57 , Issue 2 , October 1993 , pp. 287 - 292
Copyright
Copyright © British Society of Animal Science 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abdulrazak, S. A., Chen, X. B amd Órskov, E. R. 1992. The effect of supplementing ammonia-treated straw with sugar beet pulp or barley grain on rumen kinetics and microbial protein production. Animal Production 54: 505–506 (abstr.).Google Scholar
Agricultural Research Council. 1984. The nutrient requirements of ruminant livestock. Supplement no 1. Commonwealth Agricultural Bureaux, Slough.Google Scholar
Andersen, P. E., Kristensen, F. and Hermansen, J. 1989. The use of treated straw for feeding dairy cattle in Scandinavia. In Evaluation of straw in ruminant feeding (ed. Chenost, M. and Reiniger, P.), pp. 86–97. Commission of the European Communities, Elsevier.Google Scholar
Antoniewicz, A. M., Heineman, W. W. and Hanks, E. M. 1980. The effects of changes in the intestinal flow of nucleic acid on allantoin excretion in the urine of sheep. Journal of Agricultural Science, Cambridge 95: 395–400.CrossRefGoogle Scholar
Antoniewicz, A. M., Heineman, W. W. and Hanks, E. M. 1981. Effect of level of feed intake and body mass on allantoin excretion and the allantoin to creatinine ratio in the urine of sheep. Roczniki Naukowe Zootechniki T. 8: 49–65.Google Scholar
Balcells, J., Guada, J. A., Castrillo, C. and Gasa, J. 1991. Urinary excretion of allantoin and allantoin precursors by sheep after different rates of purine infusion into the duodenum. Journal of Agricultural Science, Cambridge 116: 309–317.Google Scholar
Balcells, J., Guada, J. A., Castrillo, C. and Gasa, J. 1993. Rumen digestion and urinary excretion of purine derivatives in response to urea supplementation of sodium treated straw fed to sheep. British Journal Nutrition 69: 721–732.Google Scholar
Chen, X. B., Chen, Y. K., Franklin, M. F., Órskov, E. R. and Shand, W. J. 1992. The effect of feed intake and body weight on purine derivative excretion and microbial protein supply in sheep. Journal of Animal Science 70:1534–1542.CrossRefGoogle ScholarPubMed
Chen, X. B., Hovell, F. D. de B., Órskov, E. R. and Brown, D. S. 1990. Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivatives excretion by sheep. British Journal Nutrition 63: 131–142.CrossRefGoogle ScholarPubMed
Dewhurst, R. J. and Webster, A. F. J. 1992. A note on the effect of plane of nutrition on fractional outflow rate from the rumen and urinary allantoin excretion by wether sheep. Animal Production 54: 445–448.Google Scholar
Fondevila, M. 1991. [Effect of source of carbohydrate and level of supplementation upon voluntary intake and digestibility of non-treated and ammonia treated barley straw fed sheep.] Tesis Doctoral, University of Zaragoza.Google Scholar
Fujihara, T., Órskov, E. R., Reeds, P. J. and Kyle, D. J. 1987. The effect of protein infusion on urinary excretion of purine derivatives in ruminants nourished by intragastric nutrition. Journal of Agricultural Science, Cambridge 109: 7–12.CrossRefGoogle Scholar
Giesekce, D., Stangassiner, M. and Tiemeyer, W. 1984. Nucleic acid digestion and urinary purine metabolite in sheep nourished by intragastric nutrition. Canadian Journal of Animal Science 64: suppl., pp. 144–145.Google Scholar
Giraldez, J. 1992. [Urinary excretion of catabolites in relation to variations in dietary supply of energy and nitrogen in ewe.] Tesis Doctoral, University of Leon.Google Scholar
Henning, P. A., Van Der Linden, Y., Mattheysen, M. E., Nauhaus, W. K. and Schwartz, H. M. 1980. Factors affecting the intake and digestion of roughage by sheep fed maize straw supplemented with maize grain. Journal of Agricultural Science, Cambridge 94: 565–573.CrossRefGoogle Scholar
Lindberg, J. E. 1985. Urinary allantoin excretion and digestible organic matter intake in dairy goats. Swedish Journal of Agricultural Research 15: 31–37.Google Scholar
Lindberg, J. E. 1991. Nitrogen and purine metabolism in preruminant and ruminant goat kids given increasing amounts of ribonucleic acids. Animal Feed Science and Technology 35: 213–226.CrossRefGoogle Scholar
McAllan, A. B. and Smith, R. H. 1973. Degradation of nucleic acid derivatives by rumen bacteria in vitro. British journal Nutrition 29: 467–474.Google Scholar
Nicholson, J. W. C. 1984. Digestibility, nutritive value and feed intake. In Straw and other fibrous by-products as feed (ed. Sundstol, F. and Owen, E.), pp. 340–367. Elsevier.Google Scholar
Órskov, E. R., Reid, G. W., Holland, S. M., Tait, C. A. G. and Lee, N. Y. 1988. The feeding value for ruminants of straw and whole crop barley and oat treatment with anhydrous or aqueous ammonia or urea. Animal Feed Science and Technology 8: 247–257.Google Scholar
Steel, R. G. and Torrie, H. J. 1960. Principles and procedures of statistics. McGraw-Hill, New York.Google Scholar
Storm, E. and Órskov, E. R. 1983. The nutritive value of rumen microorganism in ruminants. 1. Large-scale isolation and chemical composition of rumen microorganisms. British Journal Nutrition 50: 463–470.CrossRefGoogle Scholar
Technicon Instruments Co. 1972. Technicon clinical methods no 01. Technicon Instruments, Basingstoke.Google Scholar
Young, E. G. and Conway, C. F. 1942. On the estimation of allantoin by the Rimini-Schryver reaction. Journal of Biological Chemistry 142: 839–852.CrossRefGoogle Scholar