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Freezing buck semen diluted in amine-organic buffers

Published online by Cambridge University Press:  02 September 2010

S. Dunner
S. Dunner
Affiliation:
Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
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Abstract

Amine-organic buffers (BESKOH and TESTRIS) were compared for their ability to be used for buck semen dilution in a freezing procedure. BESKOH showed better results (P < 0·05) at the time of dilution and after chilling at +5°C (72% motility v. 54% at dilution and 62% v. 39% after chilling). After freezing and thawing, none of the variables measured (motility, normal acrosome ridge and normal swelling) was significantly different. A washing procedure was necessary when freezing was undertaken and egg yolk addition was necessary to avoid cold shock as the temperature lowered during chilling. There were no significant differences between a 3% and 12% level of egg yolk addition.

Keywords

amine-organic buffersdairy goatsegg yolksemen preservation
Type
Research Article
Information
Animal Production , Volume 56 , Issue 3 , June 1993 , pp. 387 - 391
Copyright
Copyright © British Society of Animal Science 1993

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References

Corteel, J. M. 1974. [Viability of buck spermatozoa preserved and frozen with or without their seminal plasma: glucose effect]. Annales de Biologie Animate, Biochimie et Biophysique 14 (4-B): 741–745CrossRefGoogle Scholar
Corteel, J. M. 1975. [Washing effect on buck spermatozoa preservation at low temperatures.] Annales de Biologie Animale, Biochimie et Biophysique 15: 525–528.CrossRefGoogle Scholar
Corteel, J. M. 1980. [Seminal plasma effects on spermatozoa survival and fertility when conserved in vitro.] Reproduction Nutrition Development 20: 1111–1123.CrossRefGoogle ScholarPubMed
Corteel, J. M., Leboeuf, B. and Baril, G. 1988. Artificial breeding of adult goats and kids induced with hormones to ovulate outside the breeding season. Small Ruminant Research 1: 19–35.CrossRefGoogle Scholar
Courot, M. and Volland-Nail, P. 1991. [Domestic mammals reproduction: present and future.] INRA Productions Animates 4: 21–29.CrossRefGoogle Scholar
Dunner, S. and Impastato, M. 1993. Effects of amine-organic buffers for semen dilution on fertility of does. Small Ruminant Research In press.Google Scholar
Dunner, S. and Vazquez, I. 1991. [Effects of amine-organic based extenders on buck semen quality measured in vitro after chilling at +15°C or 5+C] Archivos de Zootecnia 40: (149), 391–401.Google Scholar
Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S. and Singh, M. M. 1966. Hydrogen ion buffers for biological research. Biochemistry 2: 467–477.CrossRefGoogle Scholar
Graham, E. F., Crabo, B. G. and Brown, K. I. 1972. Effect of some zwitter ion buffers on the freezing and storage of spermatozoa. I. Bull. Journal of Dairy Science 55: 372–378.CrossRefGoogle ScholarPubMed
Graham, E. F., Schmehl, M. L. and Deyo, R. C. M. 1984. Cryopreservation and fertility of fish, poultry and mammalian spermatozoa. Proceedings of the tenth technical conference on A.I. and reproduction, Columbia, Missouri.Google Scholar
Iritani, A. and Nishikawa, Y. 1962. Studies on the egg-yolk coagulating factor (enzyme) in goat semen. III. Coagulation action to the fractionated yolk. Veterinary Zootechnology 17: 322–325.Google Scholar
Jeyendran, R. S., van der Ven, H. H., Perez Pelaez, M., Crabo, B. G. and Zaneveld, L. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal of Reproduction and Fertility 70: 219–228.CrossRefGoogle ScholarPubMed
Jones, C. and Foote, R. H. 1972. Effect of osmolality and phosphate, tris, tes, mes and hepes hydrogen ion buffers on the motility of bull spermatozoa stored at 37 or 5°C. Australian Journal of Biological Science 25: 1047–1055.CrossRefGoogle ScholarPubMed
Memon, M. A., Bretzlaff, K. N. and Ott, R. S. 1985. Effect of washing on motility and acrosome morphology of frozen-thawed goat spermatozoa. American Journal of Veterinary Research 46: 473–475.Google ScholarPubMed
Parrish, J. J. and Foote, R. H. 1980. Organic buffer for freezing spermatozoa. Journal of Animal Science 51: suppl., p. 504.Google Scholar
Pursell, V. G. and Jonhson, L. A. 1974. Glutaraldehyde fixations of boar spermatozoa for acrosome evaluation. Theriogenology 1: 638–641.Google Scholar
Roy, A. 1957. Egg-yolk coagulating enzyme in semen and Cowper's gland of the goat. Nature, London 179: 318–319.CrossRefGoogle ScholarPubMed
Salamon, S. and Ritar, A. J. 1982. Deep freezing of Angora goat semen: effects of diluent composition and method and rate of dilution on survival of spermatozoa. Australian Journal of Biological Sciences 35: 295–303.CrossRefGoogle ScholarPubMed
Statistical Analysis Systems Institute. 1988. SAS/STATm® user's guide. 5th ed. SAS Institute Inc., Cary, NC.Google Scholar
Zavos, P. and Graham, E. 1982. Effects of various degrees of supercooling on motility and fertility of turkey spermatozoa. Cryo-letters 3: 77–87.Google Scholar