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Effects of sow nutrition on maternal and foetal serum growth factors and on foetal myogenesis

Published online by Cambridge University Press:  09 March 2007

P. M. Nissen ,
I. L. Sørensen ,
M. Vestergaard  and
N. Oksbjerg
P. M. Nissen*
Affiliation:
Department of Food Science, Danish Institute of Agricultural Sciences, PO Box 50, 8830 Tjele, Denmark
I. L. Sørensen
Affiliation:
Department of Food Science, Danish Institute of Agricultural Sciences, PO Box 50, 8830 Tjele, Denmark
M. Vestergaard
Affiliation:
Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, PO Box 50, 8830 Tjele, Denmark
N. Oksbjerg
Affiliation:
Department of Food Science, Danish Institute of Agricultural Sciences, PO Box 50, 8830 Tjele, Denmark
*
†E-mail: [email protected]
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Abstract

The objective of this study was to examine the effect of increased maternal nutrition in early to mid gestation on changes in serum growth factors of the sow and foetuses. Furthermore, the effect of the foetal sera on in vitro proliferation and differentiation of porcine primary myoblasts was examined. Pregnant sows were either given food either in accordance to requirements (2 kg/day; C) until day 50 or 70 of gestation or given food in accordance to requirements until day 25 and then ad libitum (A) until day 50 or 70. Sows were slaughtered at the Institute's veterinary controlled slaughterhouse at day 50 or 70, respectively. Serum from sows and pools of cord-blood serum from each litter were analysed for glucose, lactate, insulin, insulin-like growth factor (IGF; IGF-1 and -2) and IGF binding proteins (IGFBPs). IGF-1 (P < 0·001) was higher in A compared with C sows, and a 28-kDa IGFBP (P < 0·05) and a 24-kDa IGFBP (P < 0·05) was higher in serum from day 70 compared with day 50 sows. There was no significant effect of food intake on growth factor concentrations in foetal serum or on serum-induced proliferation and differentiation of myoblasts. A 28 kDa IGFBP, IGFBP-2 and -3 were all higher (P < 0·06) and serum-induced proliferation (P < 0·001) and differentiation (P < 0·1) lower at day 70 than day 50. Maternal food intake did not influence the DNA and RNA concentrations and the CPK activity in the foetal longissimus dorsi muscle. The glucose concentration in the liver was higher in C than A foetuses at day 70 of gestation, but not at day 50.

In conclusion, no significant effects of maternal nutrition were found on serum growth factor concentrations in the foetuses or on serum-induced proliferation and differentiation of primary myoblasts.

Keywords

insulin-like growth factor (IGF)IGF binding proteinmaternal nutritionmyogenesispigs
Type
Research Article
Information
Animal Science , Volume 80 , Issue 3 , June 2005 , pp. 299 - 306
Copyright
Copyright © British Society of Animal Science 2005

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