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Prolonged administration of a β-agonist, salbutamol, to lambs does not impair metabolic or endocrine responses to the stress of dipping

Published online by Cambridge University Press:  02 September 2010

J. M. Bassett ,
A. S. Bowman ,
C. Hanson ,
R. G. Rodway  and
P. Speed
J. M. Bassett
Affiliation:
Growth and Development Unit, University Field Laboratory, Wytham, Oxford OX2 8QJ
A. S. Bowman
Affiliation:
Growth and Development Unit, University Field Laboratory, Wytham, Oxford OX2 8QJ
C. Hanson
Affiliation:
Growth and Development Unit, University Field Laboratory, Wytham, Oxford OX2 8QJ
R. G. Rodway
Affiliation:
Department of Animal Physiology and Nutrition, University of Leeds, Leeds LS2 9JT
P. Speed
Affiliation:
Growth and Development Unit, University Field Laboratory, Wytham, Oxford OX2 8QJ
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Abstract

Previous studies in foetal and growing sheep have shown that prolonged administration of β-agonist drugs leads to selective but marked attenuation of cardiovascular, metabolic and endocrine responses to the natural catecholamine hormones adrenaline and noradrenaline, as well as attenuation of responses to the drug itself. The experiment reported here was carried out to determine whether administration of the β-agonist drug, salbutamol, by twice-daily intramuscular injection at rates of 40 or 200 μ/kg per day, for a period long enough to result in substantial attenuation of its metabolic and endocrine effects, might also result in impairment in the ability of treated animals to respond to stressful husbandry procedures which disturb metabolic homeostasis and which depend on increased sympathetic nervous and adrenal activity for the restoration of homeostasis. Serial blood sampling on day 1 and day 14 of salbutamol treatment showed that the large increases in plasma lactate and glucose observed on the 1st day were absent on day 14, while the rapid increases in free fatty acids and insulin observed on day 1 were very greatly attenuated. Daily blood sampling also demonstrated that salbutamol, like cimaterol, significantly decreased both pre- and post-feeding plasma insulin and glucose concentrations.

Metabolic and endocrine changes consequent on herding and immersion of the lambs in a sheep-dip, were unaltered by 14 days of salbutamol treatment, even though the procedure resulted in activation of the pituitary-adrenal axis as evidenced by increased β-endorphin and cortisol concentrations, and a large increase in plasma lactate concentration. These results suggest β-agonist-treated animals can respond normally to physiological stresses but further investigations remain necessary to determine whether responses dependent on increased lipid mobilization and shivering, such as shearing or prolonged exposure to severe cold, remain normal in β-agonist treated animals.

Keywords

β-agonistshormoneslambsmetabolitesstress response
Type
Research Article
Information
Animal Production , Volume 56 , Issue 1 , February 1993 , pp. 107 - 114
Copyright
Copyright © British Society of Animal Science 1993

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References

Bassett, J. M. 1970. Metabolic effects of catecholamines in sheep. Australian journal of Biological Sciences 23: 903–914.CrossRefGoogle Scholar
Bassett, J. M. 1989. Hormones and metabolic adaptation in the newborn. Proceedings of the Nutrition Society 48: 263–269.CrossRefGoogle ScholarPubMed
Bassett, J. M. 1993. Effects of prolonged administration of a beta-agonist drug, ritodrine, on cardiovascular, metabolic and endocrine responses to adrenaline in growing ram lambs. Journal of Agricultural Science In press.Google Scholar
Bassett, J. M., Burks, A. H., Levine, D. H., Pinches, R. A. and Visser, G. H. A. 1985. Maternal and fetal metabolic effects of prolonged ritodrine infusion. Obstetrics and Gynecology 66: 755–761.Google ScholarPubMed
Bassett, J. M., Hanson, C. and Weeding, C. M. 1989. Metabolic and cardiovascular changes during prolonged ritodrine infusion in fetal lambs. Obstetrics and Gynecology 73: 117–122.Google ScholarPubMed
Bassett, J. M., Weeding, C. M. and Hanson, C. 1990. Desensitization of β-receptor mediated responses to epinephrine in fetal lambs by prolonged ritodrine administration. Pediatric Research 28: 388–393.CrossRefGoogle ScholarPubMed
Beermann, D. H., Butler, W. R., Hogue, D. E., Fishell, V. K., Dalrymple, R. H., Ricks, C. A. and Scanes, C. G. 1987. Cimaterol-induced muscle hypertrophy and altered endocrine status in lambs. Journal of Animal Science 65: 1514–1524.CrossRefGoogle ScholarPubMed
Blum, J. W. and Flueckiger, N. 1988. Early metabolic and endocrine effects of perorally administered β-adrenoceptor agonists in calves. European Journal of Pharmacology 151: 177–187.CrossRefGoogle ScholarPubMed
Challiss, R. A. J., Budohoski, L., Newsholme, E. A., Sennitt, M. V. and Cawthorne, M. A. 1985. Effect of a novel thermogenic β-adrenoceptor agonist (BRL 26830) on insulin resistance in soleus muscle from obese Zucker rats. Biochemical and Biophysical Research Communications 128: 928–935.CrossRefGoogle Scholar
Ebling, F. J. P. and Lincoln, G. A. 1987. β-endorphin secretion in rams related to season and photoperiod. Endocrinology 120: 809–818.CrossRefGoogle Scholar
Eisemann, J. H., Huntington, G. B. and Ferrell, C. L. 1988. Effects of dietary clenbuterol on metabolism of the hindquarters in steers. Journal of Animal Science 66: 342–353.CrossRefGoogle ScholarPubMed
Fordham, D. P., Lincoln, G. A., Ssewannyana, E. and Rodway, R. G. 1989. Plasma β-endorphin and cortisol concentrations in lambs after handling, transport and slaughter. Animal Production 49: 103–107.Google Scholar
Fordham, D. P., Al-Gahtani, S., Durotoye, L. A. and Rodway, R. G. 1991. Changes in plasma cortisol and β-endorphin concentrations and behaviour in sheep subjected to a change of environment. Animal Production 52: 287–296.Google Scholar
Jephcott, E. H., McMillen, I. C., Rushen, J. and Thorburn, G. D. 1987. A comparison of the effects of electroimmobilization and/or shearing procedures on ovine plasma concentrations of β-endorphin/β-lipotrophin and cortisol. Research in Veterinary Science 43: 97–100.CrossRefGoogle ScholarPubMed
Lindsay, D. B., Hunter, R. A. and Sillence, M. N. 1992. The use of non-peptide hormones and analogues to manipulate animal performance. In The control of fat and lean deposition (ed. Buttery, P. J., Boorman, K. N. and Lindsay, D. B.), Butterworths, London pp. 277–298CrossRefGoogle Scholar
Maltin, C. A., Delday, M. I. and Reeds, P. J. 1986. The effect of a growth promoting drug, clenbuterol on fibre frequency and area in hind limb muscles from young male rats. Bioscience Reports 6: 293–299.CrossRefGoogle ScholarPubMed
Macrae, J. C., Skene, P. A., Connell, A., Buchan, V. and Lobley, G. E. 1988. The action of the β-agonist clenbuterol on protein and energy metabolism in fattening wether lambs. British journal of Nutrition 59: 457–465.CrossRefGoogle ScholarPubMed
Meisinger, D. J. 1989. Potential economic impact of carcass modifiers. Journal of Animal Science 67: 2150–2154.CrossRefGoogle Scholar
Oksbjerg, N., Blackshaw, A., Henckel, P., Fernández, J. A. and Agergaard, N. 1990. Alterations in protein accretion and histochemical characteristics of the m. longissimus dorsi in pigs caused by salbutamol (a β-adrenergic agonist). Acta Agriculturae Scandinavica 40: 397–401.CrossRefGoogle Scholar
Reeds, P. E. and Mersmann, H. J. 1991. Protein and energy requirements of animals treated with β-adrenergic agonists: a discussion. Journal of Animal Science 69: 1532–1550.CrossRefGoogle Scholar
Robertson, R. A. and Porte, D. 1973. Adrenergic modulation of basal insulin secretion in man. Diabetes 22: 1–8.CrossRefGoogle ScholarPubMed
Scheidegger, K., O'Connell, M., Robbins, D. C. and Danforth, E. 1984a. Effects of chronic β-receptor stimulation on sympathetic nervous system activity, energy expenditure, and thyroid hormones. journal of Clinical Endocrinology and Metabolism 58: 895.CrossRefGoogle Scholar
Scheidegger, K., Robbins, D. C. and Danforth, E. 1984b. Effects of chronic beta receptor stimulation on glucose metabolism. Diabetes 33: 1144–1149.CrossRefGoogle ScholarPubMed
Smith, S. A., Levy, A. L., Sennitt, M. V., Simson, D. L. and Cawthorne, M. A. 1985. Effects of BRL 26830, a novel β-adrenoceptor agonist, on glucose tolerance, insulin sensitivity and glucose turnover in Zucker (fa/fa) rats. Biochemical Pharmacology 34: 2425–2429.CrossRefGoogle ScholarPubMed
Staten, M. A., Matthews, D. E., Cryer, P. E. and Bier, D. M. 1987. Physiological increments in epinephrine stimulate metabolic rate in humans. American Journal of Physiology 253: E322–E330.Google ScholarPubMed
Wallace, A. L. C. and Bassett, J. M. 1970. Plasma growth hormone concentrations in sheep measured by radioimmunoassay. Journal of Endocrinology 47: 21–36.CrossRefGoogle ScholarPubMed
Warburton, D., Parton, L., Buckley, S., Cocsico, L. and Saluna, T. 1988. Effects of a β2-agonist on hepatic glycogen metabolism in the fetal lamb. Pediatric Research 24: 330–332.CrossRefGoogle Scholar
Warriss, P. D., Kestin, S. C., Rolph, T. P. and Brown, S. N. 1990. The effects of the beta-adrenergic agonist salbutamol on meat quality in pigs. Journal of Animal Science 68: 128–136.CrossRefGoogle ScholarPubMed
White, D. G., Rolph, T. P. and Wagstaff, A. J. 1989. The effects of salbutamol on blood pressure and heart rate in Large White and Pietrain-cross breeds of pig. Journal of Veterinary Pharmacology and Therapeutics 12: 179–188.CrossRefGoogle ScholarPubMed
Williams, P. E. V. 1987. The use of β-agonists as a means of altering body composition in livestock species. Nutrition Abstracts and Reviews, series B 57: 454–464.Google Scholar
Zeman, R. J., Ludeman, R., Easton, T. G. and Etlinger, J. D. 1988. Slow to fast alterations in skeletal muscle fibres caused by clenbuterol, a β2-receptor agonist. American journal of Physiology 254: E726–E732.Google Scholar