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4 - Zonal Adaptive Optical Microscopy for Deep Tissue Imaging

from Part II - Deep Tissue Microscopy

Published online by Cambridge University Press:  10 June 2019

Edited by
Joel Kubby ,
Sylvain Gigan  and
Meng Cui
Joel Kubby
Affiliation:
University of California, Santa Cruz
Sylvain Gigan
Affiliation:
Sorbonne Université and Laboratoire Kastler-Brossel
Meng Cui
Affiliation:
Purdue University, Indiana
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Summary

To use the visible fluorescent signal for wavefront sensing in scattering tissue at depth, we need to employ indirect wavefront sensing methods. In this section, we describe a pupil-segmentation AO method based on physical principles similar to those utilized by SH sensors. A zonal approach by nature, it differs from the wavefront measurement scheme in a SH sensor in that, rather than measuring the wavefront segments in parallel and thus being susceptible to tissue scattering as in a SH sensor, it measures the wavefront segments serially, making it applicable to strongly scattering samples such as the mouse brain.
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Wavefront Shaping for Biomedical Imaging , pp. 91 - 118
Publisher: Cambridge University Press
Print publication year: 2019

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