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15 - Shaped Beams for Light Sheet Imaging and Optical Manipulation

from Part VI - Shaped Beams for Light Sheet Microscopy

Published online by Cambridge University Press:  10 June 2019

Edited by
Joel Kubby ,
Sylvain Gigan  and
Meng Cui
Joel Kubby
Affiliation:
University of California, Santa Cruz
Sylvain Gigan
Affiliation:
Sorbonne Université and Laboratoire Kastler-Brossel
Meng Cui
Affiliation:
Purdue University, Indiana
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Summary

This chapter covers three main advanced themes in light sheet imaging. Firstly, we discuss the widely recognized issues of sample induced optical aberrations. In itself this is broad and topical area given the importance of probing deeper into biological systems. This requirement arises as we wish to gain functional imaging which often can only be gleamed at depths currently difficult to attain (e.g. > 1 mm). The scattering and absorptive properties of tissue compromise contrast in thick tissue. This issues is exacerbated in fluorescence light sheet microscopy for which the fluorescence excitation and detection paths do not coincide. In this chapter we discuss different approaches to adaptive aberration measurement and correction of both the illumination and the detection paths for deep tissue light sheet microscopy.
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Information
Wavefront Shaping for Biomedical Imaging , pp. 358 - 378
Publisher: Cambridge University Press
Print publication year: 2019

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