Physiological and metabolic costs of acclimation to chronic sub-lethal acid and aluminium exposure in rainbow trout

Summary

Introduction

Chronic exposure of freshwater fish to sub-lethal aluminium concentrations is frequently encountered in low pH soft water owing to the acid-induced leaching of Al from soils and sediments (Wright & Gjessing 1976; Cronan & Schofield, 1979; Dickson, 1980). It is now clear that, at pH values between 4.7 and 5.5, fish kills may be primarily due to the presence of Al rather than the H⁺ concentration per se (Schofield & Trojnar 1980; Baker & Schofield 1982). Physiological studies have demonstrated that the gills are the primary target organ of acute exposure to both acid or acid combined with sub-lethal Al concentrations (for reviews see Wood, 1989, 1992; Wood & McDonald, 1987). Ionoregulatory toxicity is a key feature of acid exposure (with or without Al present) whereas, under moderately acid conditions (pH levels greater than about 5), sub-lethal Al exposure is compounded by pronounced respiratory toxicity (Neville, 1985; Wood et al., 1988a,c; Walker, Wood & Bergman, 1988; Playle, Goss & Wood, 1989; Witters, Van Puymbroeck & Vanderbrought, 1991). The ionoregulatory and respiratory disturbances are associated with surface binding and precipitation of Al on the gills (Playle & Wood 1989, 1991; Reid, Rhem & McDonald, 1991; see also Brown & Waring, this volume). However, these disturbances are usually acute, limited to an initial ‘shock’ phase lasting 2–5 days, and followed by a slower period of acclimation (the development of increased resistance to Al), repair and various degrees of physiological recovery (McDonald & Wood, 1992). It is now apparent that not all processes can recover fully during chronic acid+Al exposure and that acclimation may bear certain physiological and metabolic costs (Wilson, Bergman & Wood, 1994a,ft; Wilson, Wood & Houlihan, 1995).