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Chapter 35 - Zygote Cryopreservation for IVF

from Section 7 - Embryo Cryopreservation

Published online by Cambridge University Press:  07 August 2023

By
Dean E. Morbeck
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

This chapter will discuss the techniques for fertilization verification for oocytes fertilized both conventionally and by intracytoplasmic sperm injection (ICSI). The formation of the pronuclei is the first indication of the fusion of male and female gametes. Practical considerations and laboratory protocols are detailed in the chapter.

Keywords

2PNcryopreservationzygote
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 255 - 262
Publisher: Cambridge University Press
Print publication year: 2023

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References

Testart, J., Lassalle, B., Belaisch-Allart, J., et al. High pregnancy rate after early human embryo freezing. Fertil Steril 1986; 46:268–72.CrossRefGoogle ScholarPubMed
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Veeck, L. L., Amundson, C. H., Brothman, L. J., et al. Significantly enhanced pregnancy rates per cycle through cryopreservation and thaw of pronuclear stage oocytes. Fertil Steril 1993; 59:1202–7.CrossRefGoogle ScholarPubMed
Damario, M., Hammitt, D., Session, D. and Dumesic, D. Embryo cryopreservation at the pronuclear stage and efficient embryo use optimizes the chance for a liveborn infant from a single oocyte retrieval. Fertil Steril 2000; 73:767–73.CrossRefGoogle ScholarPubMed
Damario, M. A., Hammitt, D. G., Galanits, T. M., Session, D. R. and Dumesic, D. A. Pronuclear stage cryopreservation after intracytoplasmic sperm injection and conventional IVF: implications for timing of the freeze. Fertil Steril 1999; 72:1049–54.CrossRefGoogle ScholarPubMed
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Glujovsky, D., Blake, D., Farquhar, C. and Bardach, A. Cleavage stage versus blastocyst stage embryo transfer in assisted reproductive technology. Cochrane Database Syst Rev 2012; 7:CD002118.Google Scholar
Morbeck, D. E. Importance of supply integrity for in vitro fertilization and embryo culture. Semin Reprod Med 2012; 30:182–90.Google ScholarPubMed
Wolff, H. S., Fredrickson, J. R., Walker, D. L. and Morbeck, D. E. Advances in quality control: mouse embryo morphokinetics are sensitive markers of in vitro stress. Hum Reprod 2013; 28:1776–82.CrossRefGoogle ScholarPubMed
Shapiro, B. S., Daneshmand, S. T., Garner, F. C., et al. Similar ongoing pregnancy rates after blastocyst transfer in fresh donor cycles and autologous cycles using cryopreserved bipronuclear oocytes suggest similar viability of transferred blastocysts. Fertil Steril 2010; 93:319–21.CrossRefGoogle ScholarPubMed
Wright, G., Wiker, S., Elsner, C., et al. Observations on the morphology of pronuclei and nucleoli in human zygotes and implications for cryopreservation. Hum Reprod 1990; 5:109–15.CrossRefGoogle ScholarPubMed

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