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Chapter 9 - Vitrification for IVF

Training Protocol

from Section 1 - Starting a New Laboratory and Training Protocols

Published online by Cambridge University Press:  07 August 2023

By
Jan Gunst ,
Katleen Hostens  and
Valerie Standaert
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

In recent years, reliable aseptic closed vitrification systems have been accepted instead of open devices and have changed the ART-cryopreservation landscape in the combat against any microbiological exposure. Still, misunderstanding on the balances in a vitrification method and inappropriate implementations expose IVF laboratories to inconsistent survival and pregnancy outcomes. The present set-up and training of a microdroplet vitrification model for human blastocysts takes into account some important quality-control factors to minimize technical variation with consistent good laboratory practice. A uniform approach of the physical environment at 37 °C was validated with a ready-to-use DMSO free vitrification kit plus Ssucrose warming kit and a closed storage device with a hole carrier, based on optimal visualization, simplicity of loading/recovery, consistent volumes and ionomeric-resin straws with secured seals. The microdroplet closed vitrification model offers safety, reliability, consistency with successful results towards benchmark survival / transfer rates (>95%) and promising day 5 vitrified blastocyst live birth rates (>30%).

Keywords

vitrificationclosed deviceaseptic cryopreservationblastocystmicrodropletDMSO37 °Cgood laboratory practicequality control
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 65 - 74
Publisher: Cambridge University Press
Print publication year: 2023

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References

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