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Chapter 33 - Trophectoderm Biopsy for Pre-implantation Genetic Testing

from Section 6 - Embryo Assessment: Morphology and Beyond

Published online by Cambridge University Press:  07 August 2023

By
Maurizio Poli ,
Catello Scarica ,
Federico Favero ,
Laura Sosa Fernandez  and
Antonio Capalbo
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Trophectoderm (TE) biopsy is the gold standard for collecting embryo specimens for pre-implantation genetic testing (PGT). Because TE biopsies contain approximately 5–10 cells they provide a more robust template for DNA amplification, increasing diagnostic efficiency and representativeness of the whole embryo chromosomal constitution compared to a single cell biopsy. TE biopsy requires superior micromanipulation skills. Technical aspects include the combination of laser technology and micromanipulation to detach the biopsied specimen from the blastocyst. TE biopsy can be achieved employing different approaches according to the blastocyst’s characteristics and laboratory setting. Biopsy tubing entails a high degree of manual skills and coordination. In order to reach a highly efficient PGT program, blastocyst culture, biopsy and tubing technique, as well as robust cryopreservation processes are required.

Keywords

PGTbiopsytrophectodermmicromanipulation
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 240 - 250
Publisher: Cambridge University Press
Print publication year: 2023

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References

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ESHRE PGT Consortium Steering Committee C, Carvalho, F., Coonen, E., et al. ESHRE PGT Consortium good practice recommendations for the organisation of PGT. Hum Reprod Open 2020; 2020(3):hoaa021.CrossRefGoogle ScholarPubMed
Cimadomo, D., Rienzi, L., Romanelli, V., et al. Inconclusive chromosomal assessment after blastocyst biopsy: prevalence, causative factors and outcomes after re-biopsy and re-vitrification. A multicenter experience. Hum Reprod 2018; 33(10):1839–46.Google Scholar

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