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Chapter 11 - Sperm Survival Assay for Quality Control in the IVF Laboratory

from Section 2 - Pre-procedure Protocols

Published online by Cambridge University Press:  07 August 2023

By
Marius Meintjes
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

The purpose of the human sperm survival assay (HSSA) is to test disposables, media and reagents for potential sources of cytotoxicity in a human assisted reproductive technology (ART) laboratories before their use in in vitro fertilization (IVF)/andrology procedures. The contact bioassay is a cornerstone of a successful and safe ART laboratory, and in many countries, the use of a contact bioassay is required by law. When using a bioassay in the laboratory, it must be useful rather than just fulfilling a legal requirement. This means that the assay should be able to detect very subtle levels of toxicity consistently without any false-negative or false-positive results. Several alternative contact bioassays have been described that may fulfil these requirements, but they are not all equally affordable, accessible and practical to execute. The use of human sperm in a contact bioassay is inexpensive and convenient, as well as invaluable for consistent quality assurance in ART laboratories. If used appropriately, the HSSA is sensitive, repeatable, readily available and each sample acts as its own control.

Keywords

quality controlsperm survivalmotilitytoxicity
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 80 - 85
Publisher: Cambridge University Press
Print publication year: 2023

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References

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