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Chapter 24 - Set-Up and Adapted Protocol for Piezo-ICSI

from Section 4 - Insemination/ICSI

Published online by Cambridge University Press:  07 August 2023

By
Kenichiro Hiraoka  and
Kiyotaka Kawai
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Piezo-ICSI is one of the intracytoplasmic sperm injection (ICSI) techniques using a piezo-actuator. This technique requires the use of a drive unit, a micropipette with a blunt-ended tip and an operation liquid. Piezo-ICSI can be applied for sperm immobilization, opening of the zona pellucida without oocyte deformation, and to semi-automatically break the oolemma membrane without the need for aspiration of cytoplasm into the injection pipette. The piezo-ICSI process is stable and easy and can contribute to standardizing the ICSI technique. In this chapter, we describe the set-up and protocol of piezo-ICSI for human oocytes.

Keywords

piezo-ICSIICSIfertilizationsperm
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 178 - 185
Publisher: Cambridge University Press
Print publication year: 2023

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References

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