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Chapter 18 - In Vitro Maturation of Immature Oocytes for IVM–IVF

from Section 3 - Gametes

Published online by Cambridge University Press:  07 August 2023

Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Most laboratories use HEPES buffer solution for oocyte retrieval because it provides pH stability through buffering action. In contrast, there are laboratories that use workstations which provide CO₂ and temperature stability during oocyte collection. When this type of workstation is used, HEPES buffer solution is not required. In laboratories working without a dedicated workstation, the recovery and processing of oocytes should be done quickly to maintain optimal oocyte temperature and pH conditions.

Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
, pp. 136 - 143
Publisher: Cambridge University Press
Print publication year: 2023

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References

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