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Chapter 8 - Embryo Biopsy for IVF

Training Protocol

from Section 1 - Starting a New Laboratory and Training Protocols

Published online by Cambridge University Press:  07 August 2023

By
Oriol Oliana ,
Cristina Hickman ,
Jonathan Lo  and
Dawn A. Kelk
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Pre-implantation genetic testing for aneuploidies (PGT-A) of embryos involves confirming the chromosomal status of the embryo through assessment of biopsied cells. Most miscarriages and failed implantations are believed to be due to abnormal chromosomal status. Possessing the correct chromosome complement increases the ability of an embryo to implant and result in a live birth. Historically, biopsies occurred at the cleavage stage. However, with more cells being able to be biopsied at the blastocyst stage, representing a smaller proportion of the embryo, blastocyst biopsy is currently regarded as a safer procedure with increased sensitivity for detecting mosaics. Therefore, as the benefits of blastocyst biopsy became more evident, blastocyst biopsy has gradually become the method of choice in most in vitro fertilization (IVF) laboratories. As a technical procedure, lack of blastocyst biopsy skills has been an obstacle for some clinics to adopt this practice. This chapter outlines a structured methodology to train a biopsy practitioner to acquire the competency to confidently perform this procedure in a consistent manner that is safe for the embryos whilst maximizing the chance of pregnancy.

Keywords

biopsyPGTtrainingembryologist
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 54 - 64
Publisher: Cambridge University Press
Print publication year: 2023

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References

Hassold, T. and Hunt, P. To err (meiotically) is human: the genesis of human aneuploidy. Nat Rev Genet. 2001; 2: 280–91.CrossRefGoogle Scholar
Sato, T., Sugiura-Ogasawara, M., Ozawa, F., et al. Preimplantation genetic testing for aneuploidy: a comparison of live birth rates in patients with recurrent pregnancy loss due to embryonic aneuploidy or recurrent implantation failure. Hum Reprod 2020; 35(1):255. http://doi.org/10.1093/humrep/dez289.CrossRefGoogle ScholarPubMed
Handyside, A. H., Kontogianni, E. H., Hardy, K., et al. Pregnancies from biopsied human preimplantation embryos sexed by Y‐specific DNA amplification. Nature 1990; 344(6268):768–70.CrossRefGoogle ScholarPubMed
Hardy, K., Martin, K. L., Leese, H. J., et al. Human preimplantation development in vitro is not adversely affected by biopsy at the 8‐cell stage. Hum Reprod 1990; 5(6):708–14.CrossRefGoogle ScholarPubMed
Fragouli, E., Alfarawati, S., Spath, K., et al. Analysis of implantation and ongoing pregnancy rates following the transfer of mosaic diploid-aneuploid blastocysts. Hum Genet 2017; 136:805–19.CrossRefGoogle ScholarPubMed
Capalbo, A., Ubaldi, F. M., Cimadomo, D., et al. Consistent and reproducible outcomes of blastocyst biopsy and aneuploidy screening across different biopsy practitioners: a multicentre study involving 2586 embryo biopsies. Hum Reprod 2016; 31(1):199–208.CrossRefGoogle ScholarPubMed
Gardner, D. K. and Schoolcraft, W. B. In vitro culture of human blastocysts, in Toward Reproductive Certainty: Fertility and Genetics Beyond, ed. Jansen, R. and Mortimer, D., pp. 378–88 (Carnforth, UK: Parthenon, 1999).Google Scholar
Kort, J. D., Lathi, R. B., Brookfield, K., et al. Aneuploidy rates and blastocyst formation after biopsy of morulae and early blastocysts on day 5. J Assist Reprod Genet 2015; 32(6):925–30.CrossRefGoogle ScholarPubMed
Zhang, S., Luo, K., Cheng, D., et al. Number of biopsied trophectoderm cells is likely to affect the implantation potential of blastocysts with poor trophectoderm quality. Fertil Steril 2016; 105(5):1222–7.CrossRefGoogle ScholarPubMed

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