Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-28T09:15:47.201Z Has data issue: false hasContentIssue false

Chapter 36 - Cryopreservation of Pre-compaction Embryos for IVF

from Section 7 - Embryo Cryopreservation

Published online by Cambridge University Press:  07 August 2023

By
Cecilia Sjöblom
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
Get access

Summary

Improvement in the success of cryopreservation has increased the use of human embryos produced from ovarian hyper-stimulation and in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI). High survival and implantation rates of frozen–thawed embryos have allowed for a decrease in the number of embryos transferred and even made way for a modern ‘freeze all’ approach, where all embryos derived from a fresh cycle are frozen and transferred in a subsequent frozen embryo transfer cycle. In recent years, cryopreservation has evolved to include vitrification, with many laboratories preferring this sometimes faster method, which also requires less complex equipment. However, slow freezing can often be more successful, cost-effective and faster than vitrification, particularly in zygotes and cleavage-stage embryos. For most vitrification approaches, the process is the same, independent of the embryo’s stage of development. Blastocyst vitrification can be applied for cleavage-stage embryos, and this chapter will focus on slow freezing of pre-compaction embryos.

Keywords

cryopreservationcleavage stagestorage
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 263 - 270
Publisher: Cambridge University Press
Print publication year: 2023

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

McLernon, D. J., Harrild, K., Bergh, C., et al. Clinical effectiveness of elective single versus double embryo transfer: meta-analysis of individual patient data from randomised trials. British Med J 2010; 341:c6945.CrossRefGoogle ScholarPubMed
Blockeel, C., Drakopoulos, P., Santos- Ribeiro, S., Polyzos, N. P. and Tournaye, H. A. fresh look at the freeze-all protocol: a SWOT analysis. Hum Reprod 2016; 31(3):491–7.CrossRefGoogle Scholar
Trounson, A. and Mohr, L. Human- pregnancy following cryopreservation, thawing and transfer of an 8-cell embryo. Nature 1983; 305(5936):707–9.CrossRefGoogle Scholar
Zeilmaker, G. H., Alberda, A. T., Vangent, I., Rijkmans, C. and Drogendijk, A. C. Pregnancies following transfer of intact frozen-thawed embryos. Fertil Steril 1984; 42(2):293–6.CrossRefGoogle ScholarPubMed
Lassalle, B., Testart, J. and Renard, J. P. Human-embryo features that influence the success of cryopreservation with use of 1,2-propanediol. Fertil Steril 1985; 44(5):645–51.CrossRefGoogle Scholar
Testart, J., Lassalle, B., Belaisch-Allart, J., et al. High pregnancy rate after early human embryo freezing. Fertil Steril 1986; 46:268–72.CrossRefGoogle ScholarPubMed
Kuleshova, L., Gianaroli, L., Magli, C., Ferraretti, A. and Trounson, A. Birth following vitrification of a small number of human oocytes: case report Hum Reprod 1999; 14(12):3077–9.Google Scholar
Yokota, Y., Sato, S., Yokota, M., et al. Successful pregnancy following blastocyst vitrification: case report. Hum Reprod 2000; 15(8):1802–3.CrossRefGoogle ScholarPubMed
Balaban, B., Urman, B., Ata, B., et al. A randomized controlled study of human day 3 embryo cryopreservation by slow freezing or vitrification: vitrification is associated with higher survival, metabolism and blastocyst formation. Hum Reprod 2008; 23(9):1976–82.CrossRefGoogle ScholarPubMed
Edgar, D. H. and Gook, D. A. A critical appraisal of cryopreservation (slow cooling versus vitrification) of human oocytes and embryos. Hum Reprod Update 2012; 18(5):536–54.Google Scholar
Edgar, D. H., Archer, J., McBain, J. and Borne, H. Embryonic factors affecting outcome from single cryopreserved embryo transfer. Reprod Biomed Online 2007; 14(6):718–23.CrossRefGoogle ScholarPubMed
Edgar, D. H., Karani, J. and Gook, D. A. Increasing dehydration of human cleavage- stage embryos prior to slow cooling significantly increases cryosurvival. Reprod Biomed Online 2009; 19:521–5.CrossRefGoogle ScholarPubMed

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×