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Chapter 7 - Sperm Function Tests

Published online by Cambridge University Press:  16 February 2022

David Mortimer ,
Lars Björndahl ,
Christopher L. R. Barratt ,
José Antonio Castilla ,
Roelof Menkveld ,
Ulrik Kvist ,
Juan G. Alvarez  and
Trine B. Haugen
David Mortimer
Affiliation:
Oozoa Biomedical Inc., Vancouver
Lars Björndahl
Affiliation:
Karolinska Institutet, Stockholm
Christopher L. R. Barratt
Affiliation:
University of Dundee
José Antonio Castilla
Affiliation:
HU Virgen de las Nieves, Granada
Roelof Menkveld
Affiliation:
Stellenbosch University, South Africa
Ulrik Kvist
Affiliation:
Karolinska Institutet, Stockholm
Juan G. Alvarez
Affiliation:
Centro ANDROGEN, La Coruña
Trine B. Haugen
Affiliation:
Oslo Metropolitan University
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Summary

Provides methods for a range of common sperm function tests that are structured as standard operating procedures (SOPs) for easy use at the bench. Methods are focussed on objectivity, robustness, standardized reporting, controlling the risk of errors, and minimizing measurement uncertainty. Includes sperm hyperactivation, acrosome reaction testing, and sperm-zone pellucida binding tests (hemi-zona and competititve binding formats). A protocol for using the sperm survival test in also provided. Limitations of the hyaluronan bibding assay, and of sperm fertilizing ability testing using zona pellucida-free hanster oocytes, are summarized.

Keywords

sperm function testingfertilizing abilityacrosome reactionhyperactivationcapacitationzona pellucidareactive oxygen speciesROSfree radicalsredox
Type
Chapter
Information
A Practical Guide to Basic Laboratory Andrology , pp. 155 - 174
Publisher: Cambridge University Press
Print publication year: 2022

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