3.1 - Hatching in vitro of oncospheres/hexacanth larvae of Hymenolepis diminuta

Summary

Aims

This exercise is designed to demonstrate:

1. The importance of various physicochemical factors in the activation and hatching of the oncosphere/hexacanth via dis-solution of the embryophore.

2. The behaviour of freshly hatched larvae.

Introduction

Hymenolepis diminuta is a tapeworm of rodents (for life cycle, see Exercise 1.14). Hymenolepis eggs (Fig. 3.1.1) contain a single oncosphere/ hexacanth larva (six hooks). These eggs are infective to the beetles Tenebrio and Tribolium.

Sources of parasite material

A small number of infected rats held in one or two institutions of higher education can provide enough tapeworm eggs to supply large practical classes run at many other institutions. Eggs remain infective in faeces for several weeks and can be supplied by post from those universities and institutions that maintain the infections. Faeces should be refrigerated on arrival. See also Additional information in Exercise 1.14.

Safety

This exercise is entirely safe for humans since the eggs are only infectious to beetles! Tapeworm eggs will have been thoroughly washed and students will normally only touch the sides of clean microscope slides. It is good laboratory practice to ensure that hand to mouth movements do not occur and that students wash their hands and use a nail-brush before leaving the laboratory.