Book contents
- Frontmatter
- Contents
- Contributors
- Preface
- Part I Types of Imaging Reporter Genes
- Part II Enhancing Reporter Gene Imaging Techniques
- Part III Imaging Instrumentations
- Part IV Current Applications of Imaging Reporter Genes
- 8 Reporter Gene Imaging of Cell Signal Transduction
- 9 Gene Therapy and Imaging of Transgene Expression in Living Subjects
- 10 Imaging Regulation of Endogenous Gene Expression in Living Subjects
- 11 Imaging Cell Trafficking and Immune Cell Activation Using PET Reporter Genes
- 12 Imaging of Reporter Genes and Stem Cells
- 13 Clinical Applications of Reporter Gene Technology
- Index
- References
9 - Gene Therapy and Imaging of Transgene Expression in Living Subjects
Published online by Cambridge University Press: 07 September 2010
Book contents
- Frontmatter
- Contents
- Contributors
- Preface
- Part I Types of Imaging Reporter Genes
- Part II Enhancing Reporter Gene Imaging Techniques
- Part III Imaging Instrumentations
- Part IV Current Applications of Imaging Reporter Genes
- 8 Reporter Gene Imaging of Cell Signal Transduction
- 9 Gene Therapy and Imaging of Transgene Expression in Living Subjects
- 10 Imaging Regulation of Endogenous Gene Expression in Living Subjects
- 11 Imaging Cell Trafficking and Immune Cell Activation Using PET Reporter Genes
- 12 Imaging of Reporter Genes and Stem Cells
- 13 Clinical Applications of Reporter Gene Technology
- Index
- References
Summary
GENE THERAPY
Broadly defined, gene therapy is the process of delivering genetic materials into a patient or cells from the patient to modify the expression of one or more genes to achieve a therapeutic outcome for the patient. Hence, a transgene delivery procedure may be intended for the following reasons: (1) to initiate the expression of the transgene in specific tissues or the entire body of the patient, (2) to reduce the expression of specific endogenous genes at a target site or the entire body of the patient, and (3) to enhance the expression of specific endogenous genes in target tissues or the entire body of the patient. The success of a gene therapy procedure depends on several factors including (1) specific cell targeting when it is necessary to cause changes in gene expression only in specific cells of the patient's body and avoid modification of gene expression in all other cells. This may be necessary to avoid side effects and deliver sufficient gene expression modifying genetic material to the target cells; (2) efficiency of transgene delivery to achieve sufficient modification of gene expression in target cells; (3) modified gene expression for sufficient time duration; and (4) lack of immune reaction to the product of the therapeutic transgene, when immune reaction will reduce efficacy or potency of the therapeutic procedure. Investigators in the field of gene therapy are working hard to solve problems associated with all of these factors.
- Type
- Chapter
- Information
- Molecular Imaging with Reporter Genes , pp. 227 - 238Publisher: Cambridge University PressPrint publication year: 2010
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