4 - Genetic Responses of Bacteria at Surfaces

Summary

Introduction

The majority of studies on biofilms are concerned with the formation and structure of biofilms (see Korber et al., Chapter 1), the direct effects of microbial activities on surfaces, such as in metal corrosion (see Hamilton, Chapter 9), dental caries (see Marsh, Chapter 18) tissue invasion, or indirect effects of biofilms on fluid frictional resistance, heat exchange across metal surfaces, substrate transformations and biocide resistance of the biofilm organisms on biomaterials implanted in human patients (see McLean et al., Chapter 16) and on water distribution pipeline surfaces (Characklis & Marshall 1990; Anwar et al. 1992). Extensive reviews have dealt with the activities of microorganisms at surfaces (Marshall 1971; Stotzky 1986; van Loosdrecht et al. 1990; Fletcher 1991), but little on the actual genetic responses of microorganisms at surfaces. The aim of this chapter is to consider the environmental conditions existing at gel–air and solid–water interfaces, as well as in biofilms, and to relate these conditions to possible genetic responses in the immobilized bacteria.

Properties of surfaces

Bacteria at surfaces or, more broadly, at interfaces are exposed to environmental conditions not found in an aqueous phase. An interface is defined, in physicochemical terms, as the boundary between two phases in a heterogeneous system. The most common interfaces to which bacteria are exposed in nature and under laboratory conditions are solid–water interfaces (stones, soil particles, ship hulls, pipeline surfaces, glass and plastic culture vessels), although air–water (surface of bodies of water, bubbles), oil–water (oil spills) and solid–air (intertidal zones, agar surfaces) interfaces also are encountered. The concept of bacterial behaviour at various interfaces has been treated in detail by Marshall (1976).