Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-27T20:25:08.907Z Has data issue: false hasContentIssue false

Chapter 3 - Current ICSI Applications and Clinical Outcomes

Published online by Cambridge University Press:  02 December 2021

By
Derek Keating ,
Stephanie Cheung ,
Philip Xie ,
June Wang ,
Zev Rosenwaks  and
Gianpiero D. Palermo
Edited by
Gianpiero D. Palermo  and
Zsolt Peter Nagy
Gianpiero D. Palermo
Affiliation:
Cornell Institute of Reproductive Medicine, New York
Zsolt Peter Nagy
Affiliation:
Reproductive Biology Associates, Atlanta, GA
Get access

Summary

Since its inception, ICSI has become the most widely used ART technique, and the ultimate treatment for severe male factor infertility. In this chapter, we provide fertilization and clinical pregnancy outcomes with ICSI utilizing oligo-, crypto- and astheno- zoospermic samples. We also describe the negative impact of ooplasmic dysmaturity on fertilization and propose methods to correct it. We also report the selection of spermatozoa with higher genomic integrity using a microfluidic chip in couples with high chromatin fragmentation and complete embryo aneuploidy. This will be followed by a discussion on the safety of ICSI in which we enlist various follow-up studies on the development and health of ICSI offspring through adulthood. To provide an overview on the widespread utilization of this procedure, we comment on the ICSI results reported worldwide.

Keywords

Intracytoplasmic sperm injectionmale infertilitysurgical sperm retrievaloocyte cytoplasmic maturityDNA fragmentationmicrofluidicsassisted oocyte activation
Type
Chapter
Information
Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
With Other Advanced Micromanipulation Techniques to Edit the Genetic and Cytoplasmic Content of the Oocyte
 , pp. 25 - 37
Publisher: Cambridge University Press
Print publication year: 2021

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Hamberger, L, Lundin, K, Sjogren, A, Soderlund, B. Indications for intracytoplasmic sperm injection. Human Reproduction 1998;13 Suppl 1: 128–133.CrossRefGoogle ScholarPubMed
Adamson, GD, de Mouzon, J, Chambers, GM, Zegers-Hochschild, F, Mansour, R, Ishihara, O, Banker, M, Dyer, S. International Committee for Monitoring Assisted Reproductive Technology: world report on assisted reproductive technology, 2011. Fertility and sterility 2018;110:1067–1080.CrossRefGoogle ScholarPubMed
De Geyter, C, Calhaz-Jorge, C, Kupka, MS, Wyns, C, Mocanu, E, Motrenko, T, Scaravelli, G, Smeenk, J, Vidakovic, S, Goossens, V et al. ART in Europe, 2014: results generated from European registries by ESHRE: The European IVF-monitoring Consortium (EIM) for the European Society of Human Reproduction and Embryology (ESHRE). Human Reproduction 2018;33:1586–1601.Google Scholar
Zagadailov, P, Hsu, A, Stern, JE, Seifer, DB. Temporal differences in utilization of intracytoplasmic sperm injection among U.S. regions. Obstetrics and Gynecology 2018;132:310–320.Google Scholar
Palermo, GD, Schlegel, PN, Sills, ES, Veeck, LL, Zaninovic, N, Menendez, S, Rosenwaks, Z. Births after intracytoplasmic injection of sperm obtained by testicular extraction from men with nonmosaic Klinefelter’s syndrome. The New England Journal of Medicine 1998;338:588–590.CrossRefGoogle ScholarPubMed
Palermo, GD, Neri, QV, Schlegel, PN, Rosenwaks, Z. Intracytoplasmic sperm injection (ICSI) in extreme cases of male infertility. PLoS One 2014;9:e113671.CrossRefGoogle ScholarPubMed
Palermo, GD, O’Neill, CL, Chow, S, Cheung, S, Parrella, A, Pereira, N, Rosenwaks, Z. Intracytoplasmic sperm injection: state of the art in humans. Reproduction 2017;154:F93–F110.CrossRefGoogle ScholarPubMed
Pereira, N, Neri, QV, Lekovich, JP, Palermo, GD, Rosenwaks, Z. The role of in-vivo and in-vitro maturation time on ooplasmic dysmaturity. Reproductive Biomedicine Online 2016;32:401–406.Google Scholar
Parrella, A, Irani, M, Keating, D, Chow, S, Rosenwaks, Z, Palermo, GD. High proportion of immature oocytes in a cohort reduces fertilization, embryo development, pregnancy and live birth rates following ICSI. Reproductive Biomedicine Online 2019;39:580–587.Google Scholar
Oleszczuk, K, Augustinsson, L, Bayat, N, Giwercman, A, Bungum, M. Prevalence of high DNA fragmentation index in male partners of unexplained infertile couples. Andrology 2013;1:357–360.CrossRefGoogle ScholarPubMed
Bungum, M, Humaidan, P, Spano, M, Jepson, K, Bungum, L, Giwercman, A. The predictive value of sperm chromatin structure assay (SCSA) parameters for the outcome of intrauterine insemination, IVF and ICSI. Human Reproduction 2004;19:1401–1408.Google Scholar
Frydman, N, Prisant, N, Hesters, L, Frydman, R, Tachdjian, G, Cohen-Bacrie, P, Fanchin, R. Adequate ovarian follicular status does not prevent the decrease in pregnancy rates associated with high sperm DNA fragmentation. Fertility and Sterility 2008;89:92–97.CrossRefGoogle Scholar
Parrella, A, Keating, D, Cheung, S, Xie, P, Stewart, JD, Rosenwaks, Z, Palermo, GD. A treatment approach for couples with disrupted sperm DNA integrity and recurrent ART failure. Journal of Assisted Reproduction and Genetics 2019;36:2057–2066.CrossRefGoogle ScholarPubMed
Neri, QV, Lee, B, Rosenwaks, Z, Machaca, K, Palermo, GD. Understanding fertilization through intracytoplasmic sperm injection (ICSI). Cell Calcium 2014;55:24–37.CrossRefGoogle ScholarPubMed
Tavalaee, M, Nomikos, M, Lai, FA, Nasr-Esfahani, MH. Expression of sperm PLCzeta and clinical outcomes of ICSI-AOA in men affected by globozoospermia due to DPY19L2 deletion. Reproductive Biomedicine Online 2018;36:348–355.CrossRefGoogle ScholarPubMed
Wolny, YM, Fissore, RA, Wu, H, Reis, MM, Colombero, LT, Ergun, B, Rosenwaks, Z, Palermo, GD. Human glucosamine-6-phosphate isomerase, a homologue of hamster oscillin, does not appear to be involved in Ca2+ release in mammalian oocytes. Molecular Reproduction and Development 1999;52:277–287.3.0.CO;2-0>CrossRefGoogle Scholar
Bonte, D, Ferrer-Buitrago, M, Dhaenens, L, Popovic, M, Thys, V, De Croo, I, De Gheselle, S, Steyaert, N, Boel, A, Vanden Meerschaut, F et al. Assisted oocyte activation significantly increases fertilization and pregnancy outcome in patients with low and total failed fertilization after intracytoplasmic sperm injection: a 17-year retrospective study. Fertility and Sterility 2019;112:266–274.Google Scholar
Mansour, R, Fahmy, I, Tawab, NA, Kamal, A, El-Demery, Y, Aboulghar, M, Serour, G. Electrical activation of oocytes after intracytoplasmic sperm injection: a controlled randomized study. Fertility and Sterility 2009;91:133–139.CrossRefGoogle ScholarPubMed
Norozi-Hafshejani, M, Tavalaee, M, Azadi, L, Bahadorani, M, Nasr-Esfahani, MH. Effects of assisted oocyte activation with calcium-ionophore and strontium chloride on in vitro ICSI outcomes. Iran Journal of Basic Medical Sciences 2018;21:1109–1117.Google Scholar
Nikiforaki, D, Vanden Meerschaut, F, de Roo, C, Lu, Y, Ferrer-Buitrago, M, de Sutter, P, Heindryckx, B. Effect of two assisted oocyte activation protocols used to overcome fertilization failure on the activation potential and calcium releasing pattern. Fertility and Sterility 2016;105:798–806 e792.Google Scholar
Ebner, T, Koster, M, Shebl, O, Moser, M, Van der Ven, H, Tews, G, Montag, M. Application of a ready-to-use calcium ionophore increases rates of fertilization and pregnancy in severe male factor infertility. Fertility and Sterility 2012;98:1432–1437.Google Scholar
Ebner, T, Oppelt, P, Wober, M, Staples, P, Mayer, RB, Sonnleitner, U, Bulfon-Vogl, S, Gruber, I, Haid, AE, Shebl, O. Treatment with Ca2+ ionophore improves embryo development and outcome in cases with previous developmental problems: a prospective multicenter study. Human Reproduction 2015;30:97–102.Google Scholar
Mateizel, I, Verheyen, G, Van de Velde, H, Tournaye, H, Belva, F. Obstetric and neonatal outcome following ICSI with assisted oocyte activation by calcium ionophore treatment. Journal of Assisted Reproduction and Genetics 2018;35:1005–1010.Google Scholar
Pereira, N, O’Neill, C, Lu, V, Rosenwaks, Z, Palermo, GD. The safety of intracytoplasmic sperm injection and long-term outcomes. Reproduction 2017;154:F61-f70.CrossRefGoogle ScholarPubMed
Ferguson, KA, Wong, EC, Chow, V, Nigro, M, Ma, S. Abnormal meiotic recombination in infertile men and its association with sperm aneuploidy. Human Molecular Genetics 2007;16:2870–2879.CrossRefGoogle ScholarPubMed
Katagiri, Y, Neri, QV, Takeuchi, T, Schlegel, PN, Megid, WA, Kent-First, M, Rosenwaks, Z, Palermo, GD. Y chromosome assessment and its implications for the development of ICSI children. Reproductive Biomedicine Online 2004;8:307–318.Google Scholar
Kent-First, MG, Kol, S, Muallem, A, Blazer, S, Itskovitz-Eldor, J. Infertility in intracytoplasmic-sperm-injection-derived sons. Lancet 1996;348:332.Google Scholar
Schieve, LA, Meikle, SF, Ferre, C, Peterson, HB, Jeng, G, Wilcox, LS. Low and very low birth weight in infants conceived with use of assisted reproductive technology. The New England Journal of Medicine 2002;346:731–737.CrossRefGoogle ScholarPubMed
Pereira, N, Cozzubbo, T, Cheung, S, Rosenwaks, Z, Palermo, GD, Neri, QV. Identifying maternal constraints on fetal growth and subsequent perinatal outcomes using a multiple embryo implantation model. PLoS One 2016;11:e0166222.CrossRefGoogle ScholarPubMed
Belva, F, Roelants, M, De Schepper, J, Van Steirteghem, A, Tournaye, H, Bonduelle, M. Reproductive hormones of ICSI-conceived young adult men: the first results. Human Reproduction 2017;32:439–446.Google Scholar
Belva, F, Bonduelle, M, Roelants, M, Michielsen, D, Van Steirteghem, A, Verheyen, G, Tournaye, H. Semen quality of young adult ICSI offspring: the first results. Human Reproduction 2016;31:2811–2820.Google Scholar
Belva, F, Roelants, M, Vloeberghs, V, Schiettecatte, J, Evenepoel, J, Bonduelle, M, de Vos, M. Serum reproductive hormone levels and ultrasound findings in female offspring after intracytoplasmic sperm injection: first results. Fertility and Sterility 2017;107:934–939.CrossRefGoogle ScholarPubMed
Hopps, CV, Mielnik, A, Goldstein, M, Palermo, GD, Rosenwaks, Z, Schlegel, PN. Detection of sperm in men with Y chromosome microdeletions of the AZFa, AZFb and AZFc regions. Human Reproduction 2003;18:1660–1665.Google Scholar
Nakamura, Y, Kitamura, M, Nishimura, K, Koga, M, Kondoh, N, Takeyama, M, Matsumiya, K, Okuyama, A. Chromosomal variants among 1790 infertile men. International Journal of Urology 2001;8:49–52.CrossRefGoogle ScholarPubMed
Oppedisano, L, Haines, G, Hrabchak, C, Fimia, G, Elliott, R, Sassone-Corsi, P, Varmuza, S. The rate of aneuploidy is altered in spermatids from infertile mice. Human Reproduction 2002;17:710–717.CrossRefGoogle ScholarPubMed
Levron, J, Aviram-Goldring, A, Madgar, I, Raviv, G, Barkai, G, Dor, J. Sperm chromosome abnormalities in men with severe male factor infertility who are undergoing in vitro fertilization with intracytoplasmic sperm injection. Fertility and Sterility 2001;76:479–484.CrossRefGoogle ScholarPubMed
Palermo, GD, Colombero, LT, Hariprashad, JJ, Schlegel, PN, Rosenwaks, Z. Chromosome analysis of epididymal and testicular sperm in azoospermic patients undergoing ICSI. Human Reproduction 2002;17:570–575.CrossRefGoogle ScholarPubMed
Rodrigo, L, Rubio, C, Peinado, V, Villamon, R, Al-Asmar, N, Remohi, J, Pellicer, A, Simon, C, Gil-Salom, M. Testicular sperm from patients with obstructive and nonobstructive azoospermia: aneuploidy risk and reproductive prognosis using testicular sperm from fertile donors as control samples. Fertility and Sterility 2011;95:1005–1012.Google Scholar
Vozdova, M, Heracek, J, Sobotka, V, Rubes, J. Testicular sperm aneuploidy in non-obstructive azoospermic patients. Human Reproduction 2012;27:2233–2239.Google Scholar
Bonduelle, M, Wilikens, A, Buysse, A, Van Assche, E, Devroey, P, Van Steirteghem, AC, Liebaers, I. A follow-up study of children born after intracytoplasmic sperm injection (ICSI) with epididymal and testicular spermatozoa and after replacement of cryopreserved embryos obtained after ICSI. Human Reproduction 1998;13 Suppl1: 196–207.Google Scholar
Halliday, J. Outcomes for offspring of men having ICSI for male factor infertility. Asian Journal of Andrology 2012;14:116–120.CrossRefGoogle ScholarPubMed
Cheung, S, Schlegel, PN, Rosenwaks, Z, Palermo, GD. Revisiting aneuploidy profile of surgically retrieved spermatozoa by whole exome sequencing molecular karyotype. PLoS One 2019;14: e0210079.CrossRefGoogle ScholarPubMed
Ferraretti, AP, Nygren, K, Andersen, AN, de Mouzon, J, Kupka, M, Calhaz-Jorge, C, Wyns, C, Gianaroli, L, Goossens, V, European IVF-Monitoring Consortium. Trends over 15 years in ART in Europe: an analysis of 6 million cycles. Human Reproduction Open 2017;2017:hox012.CrossRefGoogle Scholar
Hayashi, M, Kawaguchi, T, Durcova-Hills, G, Imai, H. Generation of germ cells from pluripotent stem cells in mammals. Reproductive Medicine and Biology 2018;17:107–114.Google Scholar
Sosa, E, Chen, D, Rojas, EJ, Hennebold, JD, Peters, KA, Wu, Z, Lam, TN, Mitchell, JM, Sukhwani, M, Tailor, RC et al. Differentiation of primate primordial germ cell-like cells following transplantation into the adult gonadal niche. Nature Communications 2018;9:5339.Google Scholar

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×