Hedgehog transduction pathway is involved in pattern formation in <span class='italic'>Drosophila melanogaster</span> tergites

doi:10.1017/CBO9780511546204.018

16 - Hedgehog transduction pathway is involved in pattern formation in Drosophila melanogaster tergites

Published online by Cambridge University Press: 11 August 2009

M. Marí-Beffa

Edited by

Manuel Marí-Beffa and

Jennifer Knight

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M. Marí-Beffa: Affiliation:
Department of Cell Biology, Genetics and Physiology, Faculty of Science, University of Málaga, 29071 Málaga, Spain
Manuel Marí-Beffa: Affiliation:
Universidad de Málaga, Spain
Jennifer Knight: Affiliation:
University of Colorado, Boulder

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Summary

OBJECTIVE OF THE EXPERIMENT Lewis Wolpert originally proposed that a gradient of a diffusible molecule could control pattern formation depending on its concentration (Wolpert, 1969). Hedgehog (Hh) is one such widely accepted morphogenetic signal. In this exercise, we will study the function of the Hh transduction pathway in the control of pattern formation during the development of tergites (the dorsal cuticle of each abdominal segment) of Drosophila melanogaster.

DEGREE OF DIFFICULTY Moderate. The experiments described are relatively easy, inexpensive and can be carried out quickly.

INTRODUCTION

Pattern formation is one of the fundamental topics in Developmental Biology. Lewis Wolpert proposed a theoretical explanation of this process in his positional information model (Wolpert, 1969). Although a previous related model was also published (von Ubisch, 1953), the positional information model has been widely applied to a variety of developing systems. On the basis of previous results from hydra (Chapter 1) and insect segments (Locke, 1959; Lawrence, 1966; Stumpf, 1966; 1968), Wolpert (1969) suggested the existence of a gradient of a diffusible substance. This diffusible substance would be differentially interpreted into positional values. Depending on its position and how each cell interpreted the concentration of the substance, a variety of cell types could then differentiate. In order to explain his model better, Wolpert (1969) proposed the French flag model (Figure 16.1). The different colours in a cellular flag would appear as the differential expression of genes induced by the concentration of a diffusible molecule, or morphogen, distributed in a gradient away from a source or organiser (Figure 16.1).

Type: Chapter
Information: Key Experiments in Practical Developmental Biology , pp. 190 - 204

DOI: https://doi.org/10.1017/CBO9780511546204.018 [Opens in a new window]

Publisher: Cambridge University Press

Print publication year: 2005

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References

Bryant, P. J., and Schneiderman, H. A. (1969). Cell lineage, growth, and determination in the imaginal leg discs of Drosophila melanogaster. Dev. Biol. 20, 263–90CrossRef Google Scholar PubMed

Chen, Y., and Struhl, G. (1996). Dual roles for Patched in sequestering and transducing Hedgehog. Cell, 87, 553–63CrossRef Google Scholar PubMed

Denef, N., Neubuser, D., Pérez, L., and Cohen, S. M. (2000). Hedgehog induces opposite changes in turnover and subcellular localization of patched and smoothened. Cell, 18, 521–31CrossRef Google Scholar

García-Bellido, A., and Merriam, J. R. (1971). Parameters of the wing imagiant disc development of Drosophila melanogaster. Dev. Biol. 24, 61–87

Ingham, P. W. (1998). Transducing Hedgehog: The story so far. EMBO J., 17, 3505–11CrossRef Google Scholar PubMed

Kopp, A, and Duncan, I. (1997). Control of cell fate and polarity in the adult abdominal segments of Drosophila by optomotorblind. Development, 124, 3715–26Google Scholar

Kopp, A., Muskavitch, M. A. T., and Duncan, I. (1997). The roles of hedgehog and engrailed in patterning adult abdominal segments of Drosophila. Development, 124, 3703–14Google Scholar PubMed

Kopp, A., and Duncan, I. (2002). Anteroposterior patterning in adult abdominal segments of Drosophila. Dev. Biol., 242, 15–30CrossRef Google Scholar PubMed

Kornberg, T. (1981). Compartments in the abdomen of Drosophila and the role of the engrailed locus. Dev. Biol., 86, 363–72CrossRef Google Scholar PubMed

Lawrence, P. (1966). Development and determination of hairs and bristles in the milkweed bug Oncopeltus fasciatus (Lygaediae, Hemiptere). J. Cell Sci., 1, 475–98Google Scholar

Lawrence, P. A., Casal, J., and Struhl, G. (1999 a). hedgehog and engrailed: Pattern formation and polarity in the Drosophila abdomen. Development, 126, 2431–9Google Scholar PubMed

Lawrence, P. A., Casal, J., and Struhl, G. (1999b). The Hedgehog morphoan and gradients of cell affinity in the abdomen of Drosophila. Development, 126, 2441–2449

Lawrence, P. A., Casal, J., and Struhl, G. (2002). Towards a model of the organisation of planar polarity and pattern in the Drosophila abdomen. Development, 129, 2749–60Google Scholar PubMed

Locke, M. (1959). The cuticular pattern in an insect, Rhodnius prolixus Sta¥l. J. Exp. Biol., 36, 459–77Google Scholar

Madhavan, M. M., and Madhavan, K. (1982). Pattern regulation in tergite of Drosophila: A model. J. Theor. Biol., 95, 731–48CrossRef Google Scholar PubMed

Marí-Beffa, M., Celis, J. F., and García-Bellido, A. (1991). Genetic and developmental analyses of chaetae pattern formation in Drosophila tergites. Roux's Arch. Dev. Biol., 200, 132–42CrossRef Google Scholar PubMed

Martin, V., Carrillo, G., Torroja, C., and Guerrero, I. (2001). The sterol-sensing domain of Patched protein seems to control smoothened activity through Patched vesicular trafficking. Curr. Biol., 11, 601–7CrossRef Google Scholar PubMed

Meinhardt, H. (1983). Cell determination boundaries as organizing regions for secondary embryonic fields. Dev. Biol., 96, 375–85CrossRef Google Scholar PubMed

Müller, H. J. (1932). Further studies on the nature and causes of gene mutations. Proc. 6th Int. Cong. Genet., 1, 213–55Google Scholar

Robertson, C. W. (1936). The metamorphosis of Drosophila melanogaster, including an accurately timed account of the principal morphological changes. J. Morphol., 59, 351–99CrossRef Google Scholar

Roseland, C. R., and Schneiderman, H. A. (1979). Regulation and metamorphosis of the abdominal histoblasts of Drosophila melanogaster. Roux's Arch. Dev. Biol., 186, 235–65CrossRef Google Scholar

Santamaría, P., and García-Bellido, A. (1972). Localization and growth pattern of the tergite anlage of Drosophila. J. Embryol. Exp. Morphol., 28, 397–417Google Scholar PubMed

Struhl, G., Barbash, D. A., and Lawrence, P. A. (1997a). Hedgehog acts by distinct gradient and signal relay mechanisms to organise cell type and polarity in the Drosophila abdomen. Development, 124, 2155–65Google Scholar

Struhl, G., Barbash, D. A., and Lawrence, P. A. (1997b). Hedgehog organises the pattern and polarity of epidermal cells in the Drosophila abdomen. Development, 124, 2143–54Google Scholar

Strutt, H., Thomas, C., Nakano, Y., Stark, D., Neave, B., Taylor, A. M., and Ingham, P. W. (2001). Mutations in the sterol-sensing domain of Patched suggest a role for vesicular trafficking in Smoothened regulation. Curr Biol., 11, 608–13CrossRef Google Scholar PubMed

Stumpf, H. F. (1966). Mechanisms by which cells measure their position within the body. Nature, 212, 430–31CrossRef Google Scholar

Stumpf, H. F. (1968). Further studies on gradient-dependent diversification in the pupal cuticle of Galleria mellonella. J. Exp. Biol., 49, 49–60Google Scholar

Xu, T., and Rubin, G. M. (1993). Analysis of genetic mosaics in developing and adult Drosophila tissues. Development, 117, 1223–37Google Scholar PubMed

Von Ubisch, L. (1953). Entwicklungsprobleme. Jena: Gustav Fischer

Wolpert, L. (1969). Positional information and the spatial pattern of cellular differentiation. J. Theor. Biol., 25, 430–1CrossRef Google Scholar PubMed