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Chapter 11 - Oocyte Retrieval and Embryo Culture

Published online by Cambridge University Press:  24 December 2019

Kay Elder  and
Brian Dale
Kay Elder
Affiliation:
Bourn Hall Clinic, Cambridge
Brian Dale
Affiliation:
Centre for Assisted Reproduction, Naples
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Summary

Every individual treatment cycle involves a number of different stages and manipulations in the laboratory, and each case must be assessed and prepared for in advance; the afternoon prior to the procedure (the day after hCG administration) is a convenient time to make the preparations. The laboratory staff should ensure that all appropriate consent forms have been signed by both partners, including consent for special procedures and storage of cryopreserved embryos. Details of any previous assisted conception treatment should be studied, including response to stimulation, number and quality of oocytes, timing of insemination, fertilization rate, embryo quality and embryo transfer procedure, and judgments regarding whether any parameters at any stage could be altered or improved in the present cycle can be assessed. The risk of introducing any infection into the laboratory via gametes and samples must be absolutely minimized: screening tests such as human immunodeficiency virus (HIV 1 and 2: Anti-HIV 1, 2) and hepatitis B (HbsAg/Anti-HBc) and C (Anti-HCV-Ab) should be confirmed, as well as any other tests indicated by the patients’ history (e.g., HTLV-I antibody, RhD, malaria, Trypanosoma cruzi, Zika virus). If donor gametes are to be used, additional tests for the donor are required: chlamydia, cytomegalovirus and a validated testing algorithm to exclude the presence of active infection with Treponema pallidum for syphilis testing.

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In-Vitro Fertilization , pp. 213 - 253
Publisher: Cambridge University Press
Print publication year: 2020

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