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Chapter 1 - Immunohistochemistry Fundamentals

Published online by Cambridge University Press:  16 June 2022

By
Enisa Hasic
Edited by
Trung Nguyen
Trung Nguyen
Affiliation:
Peter MacCallum Cancer Centre, Australia
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Summary

The immunohistochemistry assay has evolved over many years. Primarily based on an antibody binding to epitopes in tissue sections, the technology has come a long way from its earliest manifestation using direct fluorescence labels. Current polymer technology is the result of many variations and adaptations on technique throughout the years. Indeed, much of its continued development is owed to early pioneers who experimented and overcame technical boundaries. This chapter is a broad discussion about what immunohistochemistry is from the historical perspective, and the individuals who have contributed to its expansion. It includes a journey about protocol adaptations and explanations of their context and applications. This chapter provides a sound basis in which to explore the rest of the book.

Keywords

pioneersantibodyantigenpolymeravidinbiotinperoxidasechromogenchemistryimmunology
Type
Chapter
Information
Immunohistochemistry
A Technical Guide to Current Practices
 , pp. 1 - 23
Publisher: Cambridge University Press
Print publication year: 2022

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