Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-26T03:24:32.089Z Has data issue: false hasContentIssue false

Chapter 4 - Automation and Robotics with the Roche Ventana BenchMark ULTRA

Published online by Cambridge University Press:  16 June 2022

By
Ahida Batrouney
Edited by
Trung Nguyen
Trung Nguyen
Affiliation:
Peter MacCallum Cancer Centre, Australia
Get access

Summary

Roche’s answer to current immunohistochemistry automation is the Ventana BenchMark ULTRA. An evaluation of the machine’s design, operation and maintenance is provided from a user’s viewpoint. Technologies which differentiate Ventana from other manufacturers are discussed in detail. The liquid coverslip, hapten-based detection system and random access points are examples of these differences when compared to traditional coverplate and polymer technology as used by other vendors. A commentary about proprietary reagents and staining protocols are offered along with advice on achieving workflow efficiencies. Discovering quality control measures of the instrument is another topic covered. Ultimately, it is hoped the reader understands the intricacies of this platform and the advantages and disadvantages of such a system, and gains a sense of the technology behind the BenchMark ULTRA.

Keywords

liquid coverslipinstrumentationautostainerOptiViewUltraViewprotocoloptimizationvalidationvendormanufacturer
Type
Chapter
Information
Immunohistochemistry
A Technical Guide to Current Practices
 , pp. 85 - 119
Publisher: Cambridge University Press
Print publication year: 2022

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cartun, RW, Taylor, CR, Dabbs, DJ 2019. Techniques of immunohistochemistry: Principles, pitfalls and standardization. In Dabbs, DJ, ed. Diagnostic immunohistochemistry: Theranostic and genomic applications. Saunders; 1–46.Google Scholar
Conde, E, Hernandez, S, Benito, A, Caminoa, A, Garrido, P, Lopez-Rios, F 2021. Screening for ROS1 fusions in patients with advanced non-small cell lung carcinomas using the VENTANA ROS1 (SP384) rabbit monoclonal primary antibody. Expert Rev Mol Diagn 21:437–44.Google Scholar
Coons, AH, Creech, HJ, Jones, RN 1941. Immunological properties of an antibody containing a fluorescent group. Proceedings of the Society for Experimental Biology and Medicine 47(2):200–2.Google Scholar
FDA 2021. List of cleared or approved companion diagnostic devices (in vitro and imaging tools). US Food and Drug Administration (online). Available at www.fda.gov/MedicalDevices/ProductsandMedicalProcedures/InVitroDiagnostics/ucm301431.htm (Accessed 21 January 2021).Google Scholar
Lin, F, Shi, J 2015. Standardization of diagnostic immunohistochemistry. In Lin, F, Prichard, J, eds. Handbook of practical immunohistochemistry: Frequently asked questions. Springer; 17–30.Google Scholar
Myers, J 2004. Automated slide stainers for special stains, immunohistochemistry, and in situ hybridization. Medical Laboratory Observer 36(1):28–30.Google Scholar
Patel, SP, Kurzrock, R 2015. PD-L1 expression as a predictive biomarker in cancer immunotherapy. Mol Cancer Ther 14(4):847–56.Google Scholar
Prichard, J, Biting, AK 2015. Automated immunohistochemistry overview. In Lin, F, Prichard, J, eds. Handbook of practical immunohistochemistry: Frequently asked questions. Springer; 31–8.Google Scholar
Shi, RS, Key, ME, Kalra, KL 1991. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: An enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 39(6):741–8.Google Scholar
Sternberger, LA, Hardy, PH, Cuculis, JJ, Meyer, HG 1970. An unlabeled antibody enzyme method of immunohistochemistry: Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase–anti-horseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18(5):315–33.Google Scholar
Torlakovic, EE, Emilia, E, Soren, N, Mogens, V, Clive, TR 2015. Getting controls under controls: The time is now for immunohistochemistry. J Clin Pathol 68(11):879–82.CrossRefGoogle ScholarPubMed
Ventana 2011a. Protease 1. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/69d63ce1-4133-ea11-fc90-005056a71a5d?countryIsoCode=au (Accessed 24 January 2021).Google Scholar
Ventana 2011b. Protease 2. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/b9e19050-4333-ea11-fc90-005056a71a5d?countryIsoCode=au (Accessed 24 January 2021).Google Scholar
Ventana 2012. BenchMark ULTRA Advanced Staining System Operator Guide. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/feb1a30c-5c0e-e411-1db5-00215a9b0ba8?countryIsoCode=au (Accessed 21 July 2021).Google Scholar
Ventana 2014a. VENTANA Ultra Cell Conditioning Solution (ULTRA CC1). Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/87d39455-7733-ea11-fa90-005056a772fd?countryIsoCode=au (Accessed 21 July 2021).Google Scholar
Ventana 2014b. ULTRA Cell Conditioning Solution (ULTRA CC2). Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/a96b5630-7733-ea11-fa90-005056a772fd?countryIsoCode=au (Accessed 24 January 2021).Google Scholar
Ventana 2014c. Anti-ALK (D5F3) Rabbit Monoclonal Primary Antibody. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/021ed24a-2436-ea11-fc90-005056a71a5d?countryIsoCode=au (Accessed 7 July 2021).Google Scholar
Ventana 2015a. Protease 3. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/e5ff24d9-4133-ea11-fc90-005056a71a5d?countryIsoCode=gb (Accessed 24 January 2021).Google Scholar
Ventana 2015b. ISH Protease 3. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/f6384c9d-6e33-ea11-fa90-005056a772fd?countryIsoCode=au (Accessed 24 January 2021).Google Scholar
Ventana 2017. Ventana PD-L1 SP263. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/176c3abe-2936-ea11-fc90-005056a71a5d?countryIsoCode=gb (Accessed 4 July 2021).Google Scholar
Ventana 2018. Ventana PD-L1 SP142. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/55b6cde7-2536-ea11-fc90-005056a71a5d?countryIsoCode=gb (Accessed 5 July 2021).Google Scholar
Ventana 2019. Ventana ROS1 (SP384). Rabbit monoclonal primary antibody. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/b1f4ca4d-3436-ea11-fc90-005056a71a5d?countryIsoCode=gb (Accessed 3 June 2021).Google Scholar
Ventana 2020. HER2 Dual ISH DNA Probe Cocktail. Ventana Medical Systems Inc. (online). Available at https://diagnostics.roche.com/content/dam/diagnostics/us/en/LandingPages/her2-dual-ish/VENTANA-HER2-Dual-ISH-Method-Sheet.pdf (Accessed 20 July 2021).Google Scholar
Ventana 2021a. ULTRA LCS (Predilute). Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/bbd095de-7633-ea11-fa90-005056a772fd?countryIsoCode=au (Accessed 21 July 2021).Google Scholar
Ventana 2021b. UltraView Universal DAB Detection Kit. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/1a2df648-0b13-ea11-fa90-005056a772fd?countryIsoCode=au (Accessed 21 July 2021).Google Scholar
Ventana 2021c. OptiView DAB IHC Detection Kit. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/91aa0b07-1b36-ea11-fa90-005056a772fd?countryIsoCode=au (Accessed 21 July 2021).Google Scholar
Ventana 2021d. UltraView Universal Alkaline Phosphatase Red Detection Kit. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/e688db00-0b13-ea11-fa90-005056a772fd?countryIsoCode=au (Accessed 21 July 2021).Google Scholar
Ventana 2021e. ISH iVIEWBlue Detection Kit. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/e3d4d682-6e33-ea11-fa90-005056a772fd?countryIsoCode=au (Accessed 20 July 2021).Google Scholar
Ventana 2021f. Ventana Silver ISH DNP Detection Kit. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/091a97de-9662-ea11-fc90-005056a71a5d?countryIsoCode=au (Accessed 21 July 2021).Google Scholar
Ventana 2021g. Ventana Red ISH DIG Detection Kit. Ventana Medical Systems Inc. (online). Available at https://pim-eservices.roche.com/eLD/api/downloads/568df0ab-9662-ea11-fa90-005056a772fd?countryIsoCode=au (Accessed 21 July 21 2021).Google Scholar
Yaziji, H, Barry, T 2006. Diagnostic immunohistochemistry: What can go wrong? Adv Anat Pathol 13(5):238–46.Google Scholar

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×