Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-24T06:38:06.711Z Has data issue: false hasContentIssue false

16 - Vitrification of human oocytes with different tools

from Section 4 - Cryopreservation of oocytes

Published online by Cambridge University Press:  06 July 2010

By
Patrick Quinn
Edited by
Ri-Cheng Chian  and
Patrick Quinn
Ri-Cheng Chian
Affiliation:
McGill University, Montréal
Get access

Summary

This chapter focuses on the effects of using different types of a physical device, the carrier tool, to carry or enclose the cellular sample in the process of vitrification of human oocytes. It discusses the effectiveness and safety of various tools used in vitrification to hold the oocytes during cooling, storage, and warming. Cooling and warming rates play a critical role in the success of vitrification in assisted reproductive technology (ART) and, in particular, with human oocytes, which appear to be more sensitive to cryopreservation than preimplantation embryonic stages. Cryostorage is the only situation in ART where numerous samples from multiple patients are pooled together in a common liquid environment. Ethylene glycol is a standard part of all present vitrification protocols. Combinations of cryoprotectant (CPAs) have been tested, and finally a mix of ethylene glycol and dimethyl sulfoxide seems to be the most popular choice.

Keywords

cryopreservationepididymal spermintracytoplasmic sperm injectionsperm retrievalopen testicular spermextractionobstructive azoospermiamicrosurgical epididymal sperm aspiration
Type
Chapter
Information
Fertility Cryopreservation , pp. 131 - 143
Publisher: Cambridge University Press
Print publication year: 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×