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23 - Concept of human ovarian tissue cryobanking

from Section 5 - Cryopreservation of ovarian tissue

Published online by Cambridge University Press:  06 July 2010

By
Vladimir Isachenko ,
Friedrich Gagsteiger ,
Evgenia Isachenko ,
Juergen Weiss  and
Rolf Kreienberg
Edited by
Ri-Cheng Chian  and
Patrick Quinn
Ri-Cheng Chian
Affiliation:
McGill University, Montréal
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Summary

Cryopreservation of ovarian tissue from cancer patients does not simply provide the potential for restoration of fertility after cancer treatment, it also has certain therapeutic benefits. For reproductive purposes, ovarian tissue can be used in two ways: either the fresh tissue can be used to produce germinal vesicle (GV) oocytes for maturation and then cryopreservation, or the ovarian tissue can be frozen, stored, and then thawed later for transplantation or to be used as a source to generate new GV oocytes. One of the important aims of ovarian cryobanking is a restoration of reproductive function after oncological treatment. In the course of tissue cryopreservation, routine histological analysis is mandatory to minimize the risk of re-implantation of cancerous cells and, therefore, parts of the ovarian tissue are used for histology while other parts are cultured in vitro, in order to evaluate the presence of follicles.

Keywords

aseptic vitrification protocolcryoprotectantsHemi-strawvitrisafenon-aseptic embryo carrierhuman blastocysts
Type
Chapter
Information
Fertility Cryopreservation , pp. 213 - 217
Publisher: Cambridge University Press
Print publication year: 2010

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