Skip to main content Accessibility help
×
Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-20T05:38:22.580Z Has data issue: false hasContentIssue false

15 - Toward the Nondestructive Imaging of Cementum Annulations Using Synchrotron X-Ray Microtomography

from Part II - Protocols

Published online by Cambridge University Press:  20 January 2022

Stephan Naji
Affiliation:
New York University
William Rendu
Affiliation:
University of Bordeaux (CNRS)
Lionel Gourichon
Affiliation:
Université de Nice, Sophia Antipolis
Get access

Summary

In modern humans, counts of tooth cementum annulations (TCA) have been widely used to determine adult age at death using classical histology. This destructive technique requires physically thin sectioning the teeth which may not be an option for valuable fossil and subfossil specimens. For over a decade, propagation phase contrast synchrotron X-ray microtomography (SRμCT) at the European Synchrotron Radiation Facility has paved the way to non-destructively visualize dental microstructures that remain otherwise invisible to conventional X-ray absorption-based μCT. Here we extend the use of SRμCT to the non-destructive visualization of TCAs in known age archeological modern human teeth, for adult age at death estimation. Our results show that virtual sections are able to elucidate true cementum annulations even if their visibility is often close to the resolution limit of our setup. This is a promising first step in non-destructive adult age at death estimation which future technical fine-tuning will undoubtedly improve further

Type
Chapter
Information
Publisher: Cambridge University Press
Print publication year: 2022

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Boyle, Angela, Boston, Ceridwen, and Witkin, Annsofie. 2005. The Archaeological Experience at St. Luke’s Church, Old Street, Islington. (unpublished report). Oxford: Oxford Archaeology.Google Scholar
Immel, Alexander, Le Cabec, Adeline, Bonazzi, Marion, Herbig, Alexander, Temming, Heiko, Schuenemann, Verena J., Bos, Kirsten I., et al. 2016. “Effect of X-Ray Irradiation on Ancient DNA in Sub-Fossil Bones – Guidelines for Safe X-Ray Imaging.” Scientific Reports 6: 32969.CrossRefGoogle Scholar
Le Cabec, , Adeline, M. Dean, Christopher, and Begun, David R.. 2017. “Dental Development and Age at Death of the Holotype of Anapithecus hernyaki (RUD 9) Using Synchrotron Virtual Histology.” Journal of Human Evolution 108: 161–75.Google Scholar
Le Cabec, Adeline, Tang, Nancy K., Rubio, Valentin Ruano, and Hillson, Simon W.. 2019. “Non-Destructive Adult Age at Death Estimation: Visualizing Cementum Annulations in a Known Age Historical Human Assemblage Using Synchrotron X-Ray Microtomography.” American Journal of Physical Anthropology 168: 2544Google Scholar
Le Cabec, Adeline, Tang, Nancy K., and Tafforeau, Paul. 2015. “Accessing Developmental Information of Fossil Hominin Teeth Using New Synchrotron Microtomography-Based Visualization Techniques of Dental Surfaces and Interfaces.” PLoS ONE 10 (4): e0123019.CrossRefGoogle ScholarPubMed
Le Cabec, Adeline, and Toussaint, Michel. 2017. “Impacts of Curatorial and Research Practices on the Preservation of Fossil Hominid Remains.” Journal of Anthropological Sciences 95: 734.Google Scholar
Leiss-Holzinger, Elisabeth, Wiesauer, Karin, Stephani, Henrike, Heise, Bettina, Stifter, David, Kriechbaumer, Benjamin, Spachinger, Stefan J., Gusenbauer, Christian, and Withalm, Gerhard. 2015. “Imaging of the Inner Structure of Cave Bear Teeth by Novel Non-Destructive Techniques.” Palaeontologia Electronica 18 (1): 115.Google Scholar
Lieberman, Daniel E. 1994. “The Biological Basis for Seasonal Increments in Dental Cementum and Their Application to Archaeological Research.” Journal of Archaeological Science 21 (4): 525–39.Google Scholar
Newham, Elis, Robson-Brown, Kate, Gill, Pamela, and Corfe, Ian. 2017. “Sexual Dimorphism in Primate Dental Cementum Microstructure.” ISDM-IAPO Program. Bordeaux, France, 166.Google Scholar
Paganin, David., Mayo, Sherry. C., Gureyev, T. E., Miller, P. R., and Wilkins, S. W.. 2002. “Simultaneous Phase and Amplitude Extraction from a Single Defocused Image of a Homogeneous Object.” Journal of Microscopy 206 (1): 3340.Google Scholar
Smith, Tanya M., Tafforeau, Paul, Reid, Donald J., Pouech, Joane, Lazzari, Vincent, Zermeno, John P., Guatelli-Steinberg, Debbie, et al. 2010. “Dental Evidence for Ontogenetic Differences between Modern Humans and Neanderthals.” Proceedings of the National Academy of Sciences 107 (49): 20923–28.Google Scholar
Smith, Tanya M., Toussaint, Michel, Reid, Donald J., Olejniczak, Anthony J., and Hublin, Jean-Jacques. 2007. “Rapid Dental Development in a Middle Paleolithic Belgian Neanderthal.” Proceedings of the National Academy of Sciences 104: 20220–25.Google Scholar
Smith, Tanya M., Tafforeau, Paul, Le Cabec, Adeline, Bonnin, Anne, Houssaye, Alexandra, Pouech, Joane, Moggi-Cecchi, Jacopo, et al. 2015. “Dental Ontogeny in Pliocene and Early Pleistocene Hominins.” PLoS ONE 10 (2): e0118118.Google Scholar
Tafforeau, Paul, Boistel, Renaud, Boller, E., Bravin, A., Brunet, M., Chaimanee, Y., Cloetens, P., Feist, M., Hoszowska, J., and Jaeger, J. J.. 2006. “Applications of X-Ray Synchrotron Microtomography for Non-Destructive 3D Studies of Paleontological Specimens.” Applied Physics A: Materials Science & Processing 83 (2): 195202.Google Scholar
Tafforeau, Paul, and Smith, Tanya M.. 2008. “Non-Destructive Imaging of Hominoid Dental Microstructure Using Phase Contrast X-Ray Synchrotron Microtomography.” Journal of Human Evolution 54 (2): 272–78.Google Scholar
Weitkamp, Timm., Haas, David, Wegrzynek, D., and Rack, Alexander. 2011. “ANKAphase: Software for Single-Distance Phase Retrieval from Inline X-Ray Phase-Contrast Radiographs.” Journal of Synchrotron Radiation 18 (4): 617–29.CrossRefGoogle ScholarPubMed
Weitkamp, Timm., Haas, David, Wegrzynek, D., and Rack, Alexander 2013. “ANKAphase: Software for Single-Distance Phase Retrieval from Inline X-Ray Phase-Contrast Radiographs. Erratum.” Journal of Synchrotron Radiation 20 (1): 205.Google Scholar
Zabler, Simon Andreas, Riesemeier, H., Fratzl, P., and Zaslansky, P.. 2006. “Fresnel-Propagated Imaging for the Study of Human Tooth Dentin by Partially Coherent X-Ray Tomography.” Optics Express 14 (19): 8584–97.Google Scholar

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×