Book contents
- Principles of IVF Laboratory Practice
- Principles of IVF Laboratory Practice
- Copyright page
- Contents
- Contributors
- Foreword
- The Evolution of IVF Practice
- Section 1 Starting a New Laboratory and Training Protocols
- Section 2 Pre-procedure Protocols
- Section 3 Gametes
- Chapter 14 Sperm Preparation for Therapeutic IVF
- Chapter 15 Processing Surgically Retrieved Sperm in the IVF Laboratory
- Chapter 16 Cryopreservation of Sperm for IVF
- Chapter 17 Oocyte Collection for IVF
- Chapter 18 In Vitro Maturation of Immature Oocytes for IVM–IVF
- Chapter 19 Oocyte Grading by Morphological Evaluation
- Chapter 20 Vitrification of Oocytes for IVF
- Section 4 Insemination/ICSI
- Section 5 Fertilization Assessment
- Section 6 Embryo Assessment: Morphology and Beyond
- Section 7 Embryo Cryopreservation
- Section 8 Embryo Transfer
- Section 9 Quality Management
- Index
- References
Chapter 18 - In Vitro Maturation of Immature Oocytes for IVM–IVF
from Section 3 - Gametes
Published online by Cambridge University Press: 07 August 2023
Book contents
- Principles of IVF Laboratory Practice
- Principles of IVF Laboratory Practice
- Copyright page
- Contents
- Contributors
- Foreword
- The Evolution of IVF Practice
- Section 1 Starting a New Laboratory and Training Protocols
- Section 2 Pre-procedure Protocols
- Section 3 Gametes
- Chapter 14 Sperm Preparation for Therapeutic IVF
- Chapter 15 Processing Surgically Retrieved Sperm in the IVF Laboratory
- Chapter 16 Cryopreservation of Sperm for IVF
- Chapter 17 Oocyte Collection for IVF
- Chapter 18 In Vitro Maturation of Immature Oocytes for IVM–IVF
- Chapter 19 Oocyte Grading by Morphological Evaluation
- Chapter 20 Vitrification of Oocytes for IVF
- Section 4 Insemination/ICSI
- Section 5 Fertilization Assessment
- Section 6 Embryo Assessment: Morphology and Beyond
- Section 7 Embryo Cryopreservation
- Section 8 Embryo Transfer
- Section 9 Quality Management
- Index
- References
Summary
Most laboratories use HEPES buffer solution for oocyte retrieval because it provides pH stability through buffering action. In contrast, there are laboratories that use workstations which provide CO₂ and temperature stability during oocyte collection. When this type of workstation is used, HEPES buffer solution is not required. In laboratories working without a dedicated workstation, the recovery and processing of oocytes should be done quickly to maintain optimal oocyte temperature and pH conditions.
- Type
- Chapter
- Information
- Principles of IVF Laboratory PracticeLaboratory Set-Up, Training and Daily Operation, pp. 136 - 143Publisher: Cambridge University PressPrint publication year: 2023
References
Cha, K. T., Koo, J. J., Ko, J. J., et al. Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril 1991; 55:109–13.CrossRefGoogle Scholar
Trunson, A., Wood, C. and Kausche, A. In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil Steril 1994; 62(2):353–62.Google Scholar
Barnes, F. L., Crombie, A., Gardner, D. K., et al. Blastcyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. Hum Reprod 1995; 10(12):3243–7.CrossRefGoogle Scholar
Russell, J. B., Knezevich, K. M., Fabian, K. F. and Dickson, J. A. Unstimulated immature oocyte retrieval: early versus midfollicular endometrial priming. Fertil Steril 1997; 67(4):616–20.CrossRefGoogle ScholarPubMed
Lin, Y. H. and Hwang, J. L. In vitro maturation of human oocytes. Taiwan J Obstet Gynecol 2006; 45(2):95–9.CrossRefGoogle ScholarPubMed
Lim, K. S., Chae, S. J., Choo, C. W., et al. In vitro maturation: Clinical applications. Clin Exp Reprod Med 2013; 40(4):143–7.CrossRefGoogle ScholarPubMed
Farsi, M. M., Kamali, N. and Pourghasem, M. Embryological aspects of oocyte in vitro maturation. Int J Mol Cell Med 2013; 2(3):99–109.Google ScholarPubMed
Abir, R,, Ben-Aharon, I., Garor, R. et al. Cryopreservation of in vitro matured oocytes in addition to ovarian tissue freezing for fertility preservation in paediatric female cancer patients before and after cancer therapy. Hum Reprod 2016; 31(4):750–62.CrossRefGoogle ScholarPubMed
Karavani, G., Schachter-Safrai, N., Revel, A., et al. In vitro maturation rates in young premenarche patients. Fertil Steril 2019; 112(2):315–21.CrossRefGoogle ScholarPubMed
Fouks, Y., Hamilton, E., Cohen, Y., et al. In-vitro maturation of oocytes recovered during cryopreservation of pre-pubertal girls undergoing fertility preservation. Reprod Biomed Online 2020; 41(5):869–73.CrossRefGoogle ScholarPubMed
Chian, R. C., Buckett, W. M., Tulandi, T. and Tan, S. L. Prospective randomized study of human chorionic gonadotrophin priming before immature oocyte retrieval from unstimulated women with polycystic ovarian syndrome. Hum Reprod 2000; 15(1):165–70.CrossRefGoogle ScholarPubMed
Son, W. Y., Yoon, S. H. and Lim, J. H. Effect of gonadotrophin priming on in-vitro maturation of oocytes collected from women at risk of OHSS. Reprod Biomed Online 2006; 13(3):340–8.CrossRefGoogle ScholarPubMed
Soderstrom-Anttila, V., Makinen, S., Tuuri, T. and Suikkari, A. M. Favourable pregnancy results with insemination of in vitro matured oocytes from unstimulated patients. Hum Reprod 2005; 20(6):1534–40.CrossRefGoogle ScholarPubMed
Son, W. Y., Chung, J. T., Demirtas, E., et al. Comparison of in-vitro maturation cycles with and without in-vivo matured oocytes retrieved. Reprod Biomed Online 2008; 17(1):59–67.CrossRefGoogle ScholarPubMed
Son, W. Y., Chung, J. T., Chian, R. C., et al. A 38 h interval between hCG priming and oocyte retrieval increase in vivo and in vitro oocyte maturation rate in programed IVM cycles. Hum Reprod 2008; 23(9):2010–16.CrossRefGoogle ScholarPubMed
Son, W. Y. and Tan, S. L. Laboratory and embryological aspects of hCG-primed in vitro maturation cycles for patients with polycystic ovaries. Hum Reprod Update 2010; 16:675–89.Google Scholar