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Chapter D10 - Dynamic light scattering

from Part D - Hydrodynamics

Published online by Cambridge University Press:  05 November 2012

Igor N. Serdyuk ,
Nathan R. Zaccai  and
Joseph Zaccai
Igor N. Serdyuk
Affiliation:
Institute of Protein Research, Moscow
Nathan R. Zaccai
Affiliation:
University of Bristol
Joseph Zaccai
Affiliation:
Institut de Biologie Structurale, Grenoble
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Summary

Historical review

1869

J. Tyndall performed the first experimental studies on light scattering from aerosols. He explained the blue colour of the sky by the presence of dust in the atmosphere.

1871

Lord Rayleigh presented the theory of scattering from assemblies of non-interacting particles that were sufficiently small compared with the wavelength of light. According to Rayleigh, scattering by a gas occurs because of the fluctuation of the molecules around a position of equilibrium. Rayleigh obtained the formulae that explained the blue colour of the sky as being due to preferential scattering of blue light in comparison with red light by molecules in atmosphere.

1906

L. Mandelshtam raised the question on the nature of scattered light once again. He pointed out that Raylegh's arguments do not fully explain the scattering phenomenon. According to Mandelshtam light scattering is also due to the random fluctuations of molecules near the position of equilibrium. As a result of random fluctuations of order λ3, where λ is the scattering wavelength, the number of macromolecules will vary with time. It follows from the Mandelshtam theory that the translational and rotational diffusion coefficients of macromolecules could be obtained from the spectrum of the scattered light.

1924

L. Mandelshtam described theoretically and experimentally so-called combination light scattering, which was later called Raman scattering. As early as 1926 he recognised that the translational diffusion coefficient of macromolecules can be obtained from the spectrum of the light they scatter.

Type
Chapter
Information
Methods in Molecular Biophysics
Structure, Dynamics, Function
 , pp. 481 - 504
Publisher: Cambridge University Press
Print publication year: 2007

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References

Berne, B. J., and Pecora, R. (1976). Dynamic Light Scattering. New York: Wiley.Google Scholar
Benedek, G. B. (1969). Optical mixing spectroscopy in physics, chemistry, biology and engineering. In Polarization: Matière and Rayonnement, pp. 4–84. Paris: Les Presses Universitaires de France.Google Scholar
Dubin, S. B., Lunacek, J. H., and Benedek, G. B. (1967). Observation of the spectrum of light scattered by solutions of biological macromolecules. Proc. Natl. Acad. Sci. USA, 57, 1164–1171.CrossRefGoogle ScholarPubMed
Doherty, J. V., and Clarke, H. R. (1980). Noisy solutions: a source of valuable kinetic information, Sci. Prog. Oxf., 66, 385–419.Google Scholar
Chu, B. (1991). Laser Light Scattering. Basic Principles and Practice. San Diego: Academic Press.Google Scholar
Wada, A., Suda, N., Tsuda, T., and Soda, K. (1968). Rotary-diffusing broadening of Rayleigh lines scattered from optically anisotropic macromolecules in solution. J. Chem. Phys., 50, 31–35.CrossRefGoogle Scholar
Dubin, S. B., Clark, N. A., and Benedek, G. B. (1971). Measurment of the rotational diffusion coefficient of lysozyme by depolarized light scattering: configuration of lysozyme in solution. J. Chem. Phys., 54, 5158–5164.CrossRefGoogle Scholar
Seils, J., and Dorfmuller, T. H. (1991). Internal dynamics of linear and superhelical DNA as studied by photon correlation spectroscopy. Biopolymers, 31, 813–825.CrossRefGoogle ScholarPubMed
Langowski, J., Kremer, W. and Kapp, U. (1992). Dynamic light scattering for study of solution conformation and dynamics of superhelical DNA. Meth. Enzymol., 211, 431–448.Google ScholarPubMed
Eimer, W., and Pecora, R. (1991). Rotational and translational diffusion of short rodlike molecule in solution: Oligonucleotides. J. Chem Phys., 94, 2324–2329.CrossRefGoogle Scholar
Ware, B. R., and Haas, D. D. (1983). Electrophoretic Light Scattering in Fast Methods in Physical Biochemistry and Cell Biology, eds. Sha'afi, R. I. and Fernandez, S. M., Elsevier Sci. Publishers.Google Scholar
Langley, K. H., (1992). Developments in electrophoretic laser light scattering and some biochemical application. In Laser Scattering in Biochemistry, eds. Harding, S. E., Sattelle, D. B. and Bloomfield, V. A.. Cambridge: Royal Society of Chemistry.Google Scholar
Bar-Ziv, R., Meller, A., et al. (1997). Localized dynamic light scattering: probing single particle dynamics at the nanoscale. Phys. Rev. Lett., 78, 154–157.CrossRefGoogle Scholar
Meller, A., Bar-Ziv, R., et al. (1998). Localized dynamic light scattering: a new approach to dynamic measurements in optical microscopy. Biophys. J., 74, 1541–1548.CrossRefGoogle ScholarPubMed
Weissman, M., Schindler, H., and Feher, G. (1976). Determination of molecular weights by fluctuation spectroscopy: application to DNA. PNAS, 73, 2776–2780.CrossRefGoogle Scholar
Kam, Z., and Rigler, R. (1982). Cross-correlation laser scattering. Biophys. J., 39, 7–13.CrossRefGoogle ScholarPubMed
Berne, B. J., and Pecora, R. (1976). Dynamic Light Scattering. New York: Wiley.Google Scholar
Benedek, G. B. (1969). Optical mixing spectroscopy in physics, chemistry, biology and engineering. In Polarization: Matière and Rayonnement, pp. 4–84. Paris: Les Presses Universitaires de France.Google Scholar
Dubin, S. B., Lunacek, J. H., and Benedek, G. B. (1967). Observation of the spectrum of light scattered by solutions of biological macromolecules. Proc. Natl. Acad. Sci. USA, 57, 1164–1171.CrossRefGoogle ScholarPubMed
Doherty, J. V., and Clarke, H. R. (1980). Noisy solutions: a source of valuable kinetic information, Sci. Prog. Oxf., 66, 385–419.Google Scholar
Chu, B. (1991). Laser Light Scattering. Basic Principles and Practice. San Diego: Academic Press.Google Scholar
Wada, A., Suda, N., Tsuda, T., and Soda, K. (1968). Rotary-diffusing broadening of Rayleigh lines scattered from optically anisotropic macromolecules in solution. J. Chem. Phys., 50, 31–35.CrossRefGoogle Scholar
Dubin, S. B., Clark, N. A., and Benedek, G. B. (1971). Measurment of the rotational diffusion coefficient of lysozyme by depolarized light scattering: configuration of lysozyme in solution. J. Chem. Phys., 54, 5158–5164.CrossRefGoogle Scholar
Seils, J., and Dorfmuller, T. H. (1991). Internal dynamics of linear and superhelical DNA as studied by photon correlation spectroscopy. Biopolymers, 31, 813–825.CrossRefGoogle ScholarPubMed
Langowski, J., Kremer, W. and Kapp, U. (1992). Dynamic light scattering for study of solution conformation and dynamics of superhelical DNA. Meth. Enzymol., 211, 431–448.Google ScholarPubMed
Eimer, W., and Pecora, R. (1991). Rotational and translational diffusion of short rodlike molecule in solution: Oligonucleotides. J. Chem Phys., 94, 2324–2329.CrossRefGoogle Scholar
Ware, B. R., and Haas, D. D. (1983). Electrophoretic Light Scattering in Fast Methods in Physical Biochemistry and Cell Biology, eds. Sha'afi, R. I. and Fernandez, S. M., Elsevier Sci. Publishers.Google Scholar
Langley, K. H., (1992). Developments in electrophoretic laser light scattering and some biochemical application. In Laser Scattering in Biochemistry, eds. Harding, S. E., Sattelle, D. B. and Bloomfield, V. A.. Cambridge: Royal Society of Chemistry.Google Scholar
Bar-Ziv, R., Meller, A., et al. (1997). Localized dynamic light scattering: probing single particle dynamics at the nanoscale. Phys. Rev. Lett., 78, 154–157.CrossRefGoogle Scholar
Meller, A., Bar-Ziv, R., et al. (1998). Localized dynamic light scattering: a new approach to dynamic measurements in optical microscopy. Biophys. J., 74, 1541–1548.CrossRefGoogle ScholarPubMed
Weissman, M., Schindler, H., and Feher, G. (1976). Determination of molecular weights by fluctuation spectroscopy: application to DNA. PNAS, 73, 2776–2780.CrossRefGoogle Scholar
Kam, Z., and Rigler, R. (1982). Cross-correlation laser scattering. Biophys. J., 39, 7–13.CrossRefGoogle ScholarPubMed

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