Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-14T11:14:19.295Z Has data issue: false hasContentIssue false

4 - Countercurrent Chromatography for the Capture Step

Published online by Cambridge University Press:  22 September 2018

David Pfister ,
Lucrèce Nicoud  and
Massimo Morbidelli
David Pfister
Affiliation:
Ypso Facto
Lucrèce Nicoud
Affiliation:
Massachusetts Institute of Technology
Massimo Morbidelli
Affiliation:
Eidgenössische Technische Hochschule Zürich
Get access

Summary

This chapter focusses on chromatographic processes for protein capture. The first part provides a detailed description of the mechanistic phenomena involved in protein capture, which can be regarded as a non-competitive binary system protein/impurity, where the protein of interest strongly interacts with the chromatographic medium. Several multi-column counter-current processes allowing the implementation of the sequence of operations necessary in protein capture (load, wash, recovery, regeneration) in a continuous manner are presented in the second part of the chapter. Two process design approaches for multi-column capture processes are provided: an empirical one, which allows to obtain a first guess of the operating conditions from breakthrough curves experiments, and a model-based one, which allows a more rigorous determination of the process variables. The model-based approach is used to compare the performance of multi-column and single-column processes.

Keywords

captureaffinityfrontal chromatographyPCCcounter-currentmulti-columnempirical designmodel-based designbreakthroughoptimization
Type
Chapter
Information
Continuous Biopharmaceutical Processes
Chromatography, Bioconjugation, and Protein Stability
 , pp. 110 - 152
Publisher: Cambridge University Press
Print publication year: 2018

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×