Design of single-feed circularly polarized (CP) microstrip antenna is proposed in this article. The design employs the concept of E-shape patch with inclined fractal defected ground structure (IFDGS), which can improve the impedance bandwidth, gain, and axial ratio (AR) bandwidth. The excellent enhanced impedance bandwidth, axial ratio bandwidth, and gain are achieved by an inclined E-shaped fractal etched on the ground plane. The parameter studies of the E-shaped IFDGS are given to illustrate the way to obtain CP radiation. The third iterative IFDGS is fabricated on easily available FR4 substrate with a size of 0.494 λ0 × 0.494 λ0 × 0.019 λ0 (λ0 is the wavelength in free space at 3.624 GHz). The measured results verify the simulated results and show good agreement. The proposed antenna shows an impedance bandwidth of 12.7% at a centre frequency of 3.47 GHz and 3-dB AR bandwidth for this band is 2.39% at a centre frequency of 3.626 GHz. The measured peak gain for the proposed antenna is found as 8.1 dBi. The proposed antenna can be suitable for mobile WIMAX operation (IEEE 802.16e-2005 standard), wireless communication in CA-band and FCC.