Children and childhood have emerged as important topics for understanding the history of African slavery in the Americas. In historical archaeology, analyses of subadult skeletal remains have provided valuable information about the biological and social conditions of captivity, yet children are infrequently the primary subjects of study in African diaspora bioarchaeology. Recent bioarchaeological research at Hacienda La Quebrada, a late colonial sugar plantation in central Peru, brings new data to bear on these subjects. Excavations at the cemetery for the plantation's enslaved African and Afro-descendant population recovered 158 subadults ranging from newborns to 20 years old, who represented 64% of the burial sample. Paleodemographic data and skeletal indicators of stress indicate that enslaved children were disproportionately affected by the conditions of life at Hacienda La Quebrada, particularly because of insufficient diets and susceptibility to infection and disease. Although these results are specific to the context of plantation slavery in Peru's coastal sugar economy, they contribute new information about the history of African slavery in Peru and about the study of childhood in conditions of captivity and colonialism in the Spanish Americas more broadly.