Old World climbing fern [Lygodium microphyllum (Cav.) R. Br] is a smothering vine that has invaded thousands of hectares of wetlands in southern and central Florida, including the Everglades. For more than two decades, the standard management approach in natural areas has been to cut the vines at waist height, leaving climbing rachis to desiccate in the tree canopy (poodle cutting) and subsequently treat all rooted ground cover with a foliar application of a 3% v/v solution of glyphosate. While this is generally effective, there is increasing interest in providing additional control options and more selective treatments. Along with glyphosate, triclopyr is widely used in invasive plant management and may also provide increased selectivity when treating the ground cover. However, it has not been well tested on L. microphyllum, especially the more recently developed acid and choline formulations. In a series of field trials, we compared the acid, amine, and choline formulations of triclopyr against glyphosate as a positive reference and nontreated plots as a negative reference based on control of L. microphyllum at three wetland sites in southern Florida over the period of 2016 to 2020. Significant reductions in L. microphyllum cover were measured at 1 mo after treatment (MAT) and continued to the termination of the studies at 12 and 28 MAT. We found all three triclopyr formulations applied with a single-nozzle backpack sprayer at 5.4 g ae L−1 provided comparable activity to glyphosate applied at 14.4 g ae L−1. There were few differences in L. microphyllum efficacy among the three triclopyr formulations at each site. These results indicate that triclopyr is a suitable alternative to glyphosate for L. microphyllum control in wetland ecosystems. Future research should evaluate triclopyr efficacy on L. microphyllum in varied hydrologic conditions to better refine treatment prescriptions for wetlands.